Effects of Ozone on Cells in Vitro

Pace, D. M.; Landolt, Paul A.; Aftonomos, Byron Th.

doi:10.1080/00039896.1969.10665388

Cited by 47 publications

(2 citation statements)

References 10 publications

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“…Ozone gas (in medical oxygen, Ozonosan photonic, Dr. Hänsler, Iffezheim, Germany) was used at concentrations of 0.2–53 × 10 6 µ g m −3 with pure oxygen as the control (27). After incubation of the cells in 96‐well plates, medium was removed (28) and the moist cell layer exposed to the gas for 1 min within a self‐constructed glass chamber under simultaneous concentration measurement (GM‐6000‐NZL; Anseros, Tübingen, Germany).…”

Section: Methodsmentioning

confidence: 99%

Effect of ozone on oral cells compared with established antimicrobials

Huth

Jakob

Saugel³

et al. 2006

European J Oral Sciences

177

114

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Ozone has been proposed as an alternative antiseptic agent in dentistry based on reports of its antimicrobial effects in both gaseous and aqueous forms. This study investigated whether gaseous ozone (4 x 10(6) microg m(-3)) and aqueous ozone (1.25-20 microg ml(-1)) exert any cytotoxic effects on human oral epithelial (BHY) cells and gingival fibroblast (HGF-1) cells compared with established antiseptics [chlorhexidine digluconate (CHX) 2%, 0.2%; sodium hypochlorite (NaOCl) 5.25%, 2.25%; hydrogen peroxide (H(2)O(2)) 3%], over a time of 1 min, and compared with the antibiotic, metronidazole, over 24 h. Cell counts, metabolic activity, Sp-1 binding, actin levels, and apoptosis were evaluated. Ozone gas was found to have toxic effects on both cell types. Essentially no cytotoxic signs were observed for aqueous ozone. CHX (2%, 0.2%) was highly toxic to BHY cells, and slightly (2%) and non-toxic (0.2%) to HGF-1 cells. NaOCl and H(2)O(2) resulted in markedly reduced cell viability (BHY, HGF-1), whereas metronidazole displayed mild toxicity only to BHY cells. Taken together, aqueous ozone revealed the highest level of biocompatibility of the tested antiseptics.

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Section: Methodsmentioning

confidence: 99%

Effect of ozone on oral cells compared with established antimicrobials

Huth

Jakob

Saugel³

et al. 2006

European J Oral Sciences

177

114

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“…Therefore, the cell cultures on the sensor chip periodically changes from direct gas exposure to submerged conditions. This intermittent method was used of groups before 22–25. The aim of the study was to work on the basics of an in vitro gas exposure system, based on the Bionas® 2500 analyzing system as a main component, for the toxicity measurement of insoluble gases like CO, NO 2 , O 3 , and phosgene (COCl 2 ), to name but a few.…”

Section: Introductionmentioning

confidence: 99%

Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide

Bohrn

Stütz

Fleischer

et al. 2011

Physica Status Solidi (a)

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In the past years, whole cell gas toxicity assays have been developed in order to study the harmful effects of gaseous chemicals. Cells are incubated with nutrition medium supplemented with the gaseous substances to be tested. These methods are not feasible for the examination of toxic gases, which are insoluble in water. Carbon monoxide (CO), a poorly soluble and toxic gas (lethal dose 1 vol% for some minutes) has been chosen as a model gas. Besides its binding to hemoglobin, it also leads to an inhibition of cytochrome c oxidase in the mitochondrial respiratory chain of living cells. Thus, a decrease in the respiratory behavior of eukaryotic cells indicates the presence of CO in a gaseous phase. Based on the Bionas® 2500 analyzing system, a device to measure the metabolic and morphological effects, caused by direct gas exposure on eukaryotic cells was established. The gaseous substances directly contact the eukaryotic cell line V79 (Chinese hamster lung fibroblast) without interfering liquid phase in between. Beside the measurement of oxygen consumption also acidification and impedance changes of the cell culture are detected by a metabolic sensor chip. In order to increase the stability different chip surface coatings like collagen A and poly‐L‐lysin (PLL) were used. Adhesion coating leads to a tighter junction of the cells to the sensor chip surface and thus enables higher stability of the confluent cell monolayer towards the gaseous flow during the gas exposure. The method reported here has the potential to become a valuable means for rapid monitoring of toxic compounds in gaseous phases.

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Ozone Effect on Lysosomal Hydrolases of Alveolar Macrophages in Vitro

Hurst¹

1971

Arch Intern Med

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Effects of Ozone on Cells in Vitro

Cited by 47 publications

References 10 publications

Effect of ozone on oral cells compared with established antimicrobials

Effect of ozone on oral cells compared with established antimicrobials

Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide

Ozone Effect on Lysosomal Hydrolases of Alveolar Macrophages in Vitro

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