Effects of physicochemical factors and <i>in vitro</i> gastrointestinal digestion on antioxidant activity of R‐phycoerythrin from red algae <i>Bangia fusco‐purpurea</i>

Wu, Qiang; Fu, Xiaoping; Sun, Le-Chang; Zhang, Qian; Cao, Min‐Jie; Cai, Qiu-Feng

doi:10.1111/ijfs.12775

Cited by 29 publications

(15 citation statements)

References 28 publications

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“…However, there is a recent report of R-phycoerythrin (R-PE) from Bangia fusco-purpurea that gave higher values of DPPH radical scavenging activities after digestion. The reason was the formation of short peptides after digestion in which the hydrophobic amino acid residues were more exposed to DPPH radical and contributed to the overall anti-oxidant activity [ 32 ]. Among the methanol extracts SM showed a considerable decrease in the scavenging activity while PM and LM were relatively unchanged.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts

et al. 2015

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Phycobiliprotein-containing water and carotenoid-containing methanolic extracts of three different cyanobacteria, Pseudanabaena sp., Spirulina sp. and Lyngbya sp., were studied for their DPPH scavenging, iso-bolographic studies, and anti-nephrolithe activities. The best EC50 values for DPPH scavenging were in Lyngbya water (LW, 18.78 ± 1.57 mg·mg−1 DPPH) and Lyngbya methanol (LM, 59.56 ± 37.38 mg·mg−1 DPPH) extracts. Iso-bolographic analysis revealed most of the combinations of extracts were antagonistic to each other, although LM—Spirulina methanol (SM) 1:1 had the highest synergistic rate of 86.65%. In vitro digestion studies showed that DPPH scavenging activity was considerably decreased in all extracts except for Pseudanabaena methanol (PM) and LM after the simulated digestion. All of the extracts were effective in reducing the calcium oxalate crystal size by nearly 60%–65% compared to negative control, while PM and Spirulina water (SW) extracts could inhibit both nucleation and aggregation of calcium oxalate by nearly 60%–80%.

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Section: Resultsmentioning

confidence: 99%

Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts

et al. 2015

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“…In particular, the TAC values of purified C-PC and C-PE were among the highest gainers. The probable reason for this is the generation of peptides with stronger anti-oxidant activity after the digestion process, since there are more accessible amino acid residues after the digestion process (Wu et al, 2015). Similarly, digested purified lycopene and myxoxanthophyll had significantly higher activities (154.16 ± 0.96 and 134.95 ± 0.4 µg AAE ml −1 , respectively) as compared to the undigested ones; digested lycopene had the highest activity among all the samples considered.…”

Section: Total Anti-oxidant Capacity Of Undigested and Digested Cyanomentioning

confidence: 95%

“…The absorbance of the colored compound thus produced is measured at 695 nm wherein the intensity of the color is directly proportional to the anti-oxidant activity of the sample under investigation. PBPs have been reported as excellent anti-oxidant in many studies (Paliwal et al, 2015a;Wu et al, 2015). However, the digested products of the extracts and the purified pigments were found to be better anti-oxidants compared to the undigested samples.…”

Section: Total Anti-oxidant Capacity Of Undigested and Digested Cyanomentioning

confidence: 97%

“…To investigate this fact further, we digested the purified C-PE and C-PC proteins wherein we confirmed that the generation of short peptides probably plays a role in free radical scavenging; the activities of the digested samples of purified C-PE and C-PC are greater than their undigested counterparts (∼6% for purified C-PE and ∼19% for purified C-PC). The exposure of more number of amino acids to the free radical may be responsible for the increased scavenging activity (Wu et al, 2015). Similarly, in vitro digestion of carotenoids is a scarcely researched topic due to the time consuming procedures such as thin layer chromatography; however, rapid techniques like HPLC analysis have since been adapted for the identification and characterization of carotenoids (Nozière et al, 2006).…”

Section: Abts Scavenging Activity Of Undigested and Digested Cyanobacmentioning

confidence: 99%

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Cyanobacterial Pigments as Natural Anti-Hyperglycemic Agents: An In vitro Study

et al. 2016

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Traditional medicines for controlling postprandial hyperglycemia includes herbs and plant extracts as well as synthetic drugs like acarbose. Synthetic drug molecules frequently have side effects such as flatulence and diarrhea. Cyanobacterial pigments have excellent anti-oxidant and free radical scavenging properties. Thus, α-amylase and α-glucosidase inhibiting activities of purified pigments and crude extracts from three cyanobacterial species, Lyngbya, Microcoleus, and Synechocystis sp., were investigated. Lyngbya extract had the highest total anti-oxidant activity (TAC) before digestion (48.26 ± 0.04 µg AAE ml −1 ) while purified lycopene had the highest TAC after digestion (154.16 ± 0.96 µg AAE ml −1 ). The Microcoleus extract had the highest ABTS scavenging activity before digestion (98.23 ± 0.25%) while purified C-phycocyanin (C-PC) had the highest ABTS scavenging after digestion (99.69 ± 0.04%). None of the digested or undigested extracts performed better than acarbose in inhibiting α-amylase but the digested Microcoleus extract was able to inhibit its activity by ∼35%. The purified pigments gave inhibitory activities ranging from ∼8 to 16%. The Lyngbya extract had the highest inhibitory activity against α-glucosidase both before and after digestion (62.22 ± 0.02 and 97.82 ± 0.03%, respectively). Purified C-phycoerythrin (C-PE), C-PC, lycopene and myxoxanthophyll could inhibit α-glucosidase in a range of ∼83-96%. Considering the potent inhibitory activities of purified pigments against both α-amylase and α-glucosidase, cyanobacterial pigments could be used as food additives for their dual advantage of anti-oxidant and anti-hyperglycemic activities.

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“…Red algae Bangia fusco‐purpurea contains R‐Phycoerythrin (R‐PE) which has the property of antioxidant activity has been reported (Wu et al . ). This which gets increased when hydrolyzed to peptides having a molecular weight less than 3 kDa after GI digestion suggesting application for potential food.…”

Section: Introductionmentioning

confidence: 97%

Effect of Lipases fromCandida cylinderaceaon Enrichment of PUFA in Marine Microalgae

Jacob

Mathew

2016

Journal of Food Processing and Preservation

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Polyunsaturated fatty acids (PUFA) have a fundamental role in human metabolism. Here, a study was attempted to develop a commercially feasible technology to enrich eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) present in Isochrysis galbana, Chaetocerous calcitran, Chlorella marina and Tetraselmis gracillus using lipase source Candida cylinderacea. To the wet mass of algae added 4, 8 and 12 U of lipase enzyme concentration and was incubated at 37C for 4, 8, 16 and 24 h of hydrolysis. Control was taken without any added enzyme. The experiment investigated the optimum conditions of lipases as a function of time and enzyme concentrations. The study reveals that the uses of biolipases under standardized condition are useful for commercial production of PUFA in pharmaceutical industries. Practical Application Polyunsaturated fatty acids (PUFA) have a fundamental role in human metabolism due to their antibacterial, antiinflammatory and antioxidant properties, and play role in the prevention of cardiac diseases and inhibition of tumor progression. Marine fish oil is the richest source for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), but the limitations are its high susceptibility toward oxidation and consequent off‐flavors and production of toxic compounds, as well as geographical and seasonal variations in quality and quantity. As marine algae is the primary producer of n‐3 PUFA in the natural environment, replacing fish oil with n‐3 PUFA enriched algae provides an excellent opportunity to develop alternative n‐3 PUFA for nutraceutical and pharmaceutical purposes. Following this background, a study was attempted to develop a commercially feasible technology to enrich Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) present in microalgae.

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Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R‐phycoerythrin from red algae Bangia fusco‐purpurea

Cited by 29 publications

References 28 publications

Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts

Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts

Cyanobacterial Pigments as Natural Anti-Hyperglycemic Agents: An In vitro Study

Effect of Lipases fromCandida cylinderaceaon Enrichment of PUFA in Marine Microalgae

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