Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum

Monfort, Lucila Elizabeth Fragoso; Bertolucci, Suzan Kelly Vilela; Lima, A. F. de; Carvalho, Alexandre Alves de; Mohammed, Aliyu; Blank, Arie Fitzgerald; Pinto, José Eduardo Brasil Pereira

doi:10.1016/j.indcrop.2018.02.075

Cited by 58 publications

(46 citation statements)

References 44 publications

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“…The callus formation did not differ during the cultivation, presenting values close to 36%. The occurrence of callus during in vitro cultivation has been reported for many Lamiacea especies (Meftahizade et al, 2010;Bakhtiar et al, 2016;Monfort et al, 2018), demonstrating a type of responsiveness to the medium.…”

Section: Salt Concentration In the Ms Mediummentioning

confidence: 85%

“…Silva et al (2017) have verified that the MS medium at its complete strength doubled the Red Rubin basil's biomass. Monfort et al (2018) have reported a substantial increase in the number of leaves, dry and fresh masses of shoots and roots of O. basilicum in comparison with other culture media such as WPM, which contains less nitrogen, and half the MS strength (½MS).…”

Section: Salt Concentration In the Ms Mediummentioning

confidence: 99%

“…Some researchers have observed a trend in callus formation in the presence of cytokinin, isolated or combined with auxin in the culture medium (Monfort et al, 2018). The cytokinin class of growth hormones is responsible for cell multiplication and, in Arabidopsis, it functions in the expression of several transcription factors such as ESR1, ESR2, OBP1, in addition to cyclins, and CDKs (Ikeuchi et al, 2013).…”

Section: Salt Concentration In the Ms Mediummentioning

confidence: 99%

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In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

Trettel¹,

Nascimento²,

Barbosa³

et al. 2018

JAS

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Genovese basil has great economic potential; however, there is no established micropropagation protocol for this species. Therefore, this study aimed at assessing the in vitro growth of Genovese basil in response to different concentrations of salts in the Murashige and Skoog medium (MS) and interaction of sucrose and activated carbon. Two assays were conducted independently in an in vitro environment using the MS medium, regulators, agar, and five salt concentrations (0, 25, 50, 70 and 100%). In the second assay, two concentrations of sucrose (30 and 60 g L ) were tested.In addition, copper and zinc were quantified in the roots. The results showed that shoots were favored when the medium was at its full strength (100% salts), with seedlings forming more leaves.This result may be associated with a higher demand for nitrogen and because of the ionic balance between NH 4 + and NO 3 -. High concentrations of salts affected the roots, but a reduction to 70% salt favored root development. Doubling the usual dose of sucrose (60 g L -1 ) damaged the growth of the seedlings. Damage caused by osmotic and oxidative potentials, and by toxic compounds may be related to the observed results. The amount of copper and zinc in the root increased with increased concentrations of activated carbon in the medium. The presence of activated carbon reduced callus formation but did not mitigate the effects of increased sucrose concentration.

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Section: Salt Concentration In the Ms Mediummentioning

confidence: 85%

Section: Salt Concentration In the Ms Mediummentioning

confidence: 99%

Section: Salt Concentration In the Ms Mediummentioning

confidence: 99%

See 1 more Smart Citation

In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

Trettel¹,

Nascimento²,

Barbosa³

et al. 2018

JAS

View full text Add to dashboard Cite

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“…Production of secondary metabolites by plant tissue culture depends on a number of factors, chief among them are the nutrients provided for plant growth. The optimum nutrient concentration is a critical determinant in the growth of the explants and the accumulation of secondary metabolites (Rao and Ravishankar, 2002;Nagella and Murthy 2010;Murthy et al 2014;Fargoso Monfort et al 2018). The type of culture media used, the salt strength of the medium employed and the growth regulators, type and concentration used, are key factors that most be established for every culture (Rao and Ravishankar, 2002;Fargoso Monfort et al 2018).…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

“…The concentration of salts present in the culture media needed by a specific plant varies depending on the particular culture needs. The selection of a suitable medium is essential to establish cell and organ cultures (Nagella and Murthy 2010;Fargoso Monfort et al 2018). Three of the most popular culture media employed are MS, B5 and WPM, as listed in Table 4.…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

432

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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Nanoelicitors Mediated Abiotic Stresses in Plant Defense Response Mechanisms

Somkuwar Subhash,

Meshram,

Gogle

et al. 2024

Plant Secondary Metabolites and Abiotic Stress

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Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum

Cited by 58 publications

References 44 publications

In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

In vitro plant tissue culture: means for production of biological active compounds

Nanoelicitors Mediated Abiotic Stresses in Plant Defense Response Mechanisms

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