Effects of Pluronic F-68 on Tetrahymena cells: protection against chemical and physical stress and prolongation of survival under toxic conditions

Hellung‐Larsen, Per; Assaad, Fahed; Pankratova, Stanislava; Saietz, Birgitte L; Skovgaard, L. T.

doi:10.1016/s0168-1656(99)00188-1

Cited by 28 publications

(26 citation statements)

References 17 publications

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“…34 Pluronic F-68 is also used in large scale mammalian cell culture and was found that at low concentrations, can enhance cell growth. 35 Pluronic F68 is used in the external solution formulation to serve as a surfactant to prevent coalescence of the double emulsions and to improve stability of the vesicles. Ethanol serves to extract the oleic acid from the middle layer.…”

Section: Lipid Vesicle Formationmentioning

confidence: 99%

Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics

et al. 2011

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In this paper, we present a microfluidic platform for the continuous generation of stable, monodisperse lipid vesicles 20-110 lm in diameter. Our approach utilizes a microfluidic flow-focusing droplet generation design to control the vesicle size by altering the system's fluid flow rates to generate vesicles with narrow size distribution. Double emulsions are first produced in consecutive flow-focusing channel geometries and lipid membranes are then formed through a controlled solvent extraction process. Since no strong solvents are used in the process, our method allows for the safe encapsulation and manipulation of an assortment of biological entities, including cells, proteins, and nucleic acids. The vesicles generated by this method are stable and have a shelf life of at least 3 months. Here, we demonstrate the cell-free in vitro synthesis of proteins within lipid vesicles as an initial step towards the development of an artificial cell.

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Section: Lipid Vesicle Formationmentioning

confidence: 99%

Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics

et al. 2011

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“…The hypothesis under investigation was that PLF-68 increases matrix synthesis of OA-like cells besides cell viability. The survival effects of PLF-68 have been well documented for cells either mechanically traumatized (Phillips and Haut 2004;Rundell et al 2005;Baars et al 2006) or subjected to toxic and stress conditions (Hellung-Larsen et al 2000;Marks et al 2001). In the current study, it was observed that the cell viability of PLF-68 treatment groups was numerically higher than that of the negative control group in agreement with the previous findings (Hellung-Larsen et al 2000;Phillips and Haut 2004;Rundell et al 2005;Baars et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, Baars et al (2006) reported that PLF-68 reduced DNA fragmentation in cells of bovine chondral explants exposed to injurious unconfined compression. The protective effect of PLF-68 on cells against chemical and physical stress has also been documented (Hellung-Larsen et al 2000;Marks et al 2001). This surfactant forms a patch preventing the leakage of the intracellular components (Lee et al 1999;Hannig et al 2000;Padanilam et al 1994).…”

Section: Introductionmentioning

confidence: 98%

In vitro investigation and biomechanical modeling of the effects of PLF-68 on osteoarthritis in a three-dimensional model

Kavas

Özdemir

Gürses

et al. 2010

Biomech Model Mechanobiol

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In this study, it was hypothesized that Pluronic F-68 (PLF-68) increases matrix synthesis of osteoarthritis (OA) chondrocytes in addition to its well-documented cell survival effect. To test this hypothesis, rat articular chondrocytes were embedded in agarose discs and were exposed to 5-azacytidine (Aza-C) to induce OA-like alterations. Chondrocytes were then treated with PLF-68 (8 and 12 mg/ml) for 10 days. Aza-C-exposed and PLF-68-untreated chondrocytes and Aza-C-unexposed and PLF-68-untreated chondrocytes were used as negative and positive control groups, respectively. Dynamic hydrostatic pressure (max 0.2 MPa, 0.1 Hz) was applied to discs for 30 min/day (5 days/week). Cell viability, collagen and proteoglycan deposition in discs were determined. Unconfined compression stress relaxation tests were performed to determine peak stress and material parameters of discs--namely spring constants (k (1) and k (2)), damping coefficient (η), instantaneous modulus (E (0)) and relaxed modulus (E (∞)) using Kelvin model to evaluate the functional coherence of the matrix. PLF-68 treatment significantly increased the collagen deposition in discs and viability of OA-like chondrocytes. A dose-dependent increase was also observed for elastic stiffness parameters (k (1), k (2), E (0) and E (∞)). Same positive effect of PLF-68 was not observed for proteoglycan deposition. However, dose-dependent increase in η suggests that PLF-68 treatment resulted with the deposition of functional matrix. This is the first study which reports that PLF-68 has also positive effect on collagen synthesis of OA cells. As a conclusion, our results suggest that PLF-68 has a potential for recovery from OA-like alterations, which should be further analyzed.

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“…It has been observed that, besides being incorporated to the plasmatic membrane decreasing its fluidity (Palomares et al 2000), it can enter and accumulate within the cells (Gigout et al 2008). The wide range of cellular processes that this molecule can trigger makes its influence on the cellular metabolism dependent on the cell line and on the concentration used (Elibol 1999;Lee and Kim 2002;Hellung-Larsen et al 2000). It is possible that the rest of non ionic additives (PVA and PVP), not as widely studied as PF68, at high concentrations have an effect similar to that observed with PF68.…”

Section: Influence Of the Protective And Disaggregant Additives On Thmentioning

confidence: 99%

Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV)

et al. 2011

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As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for growing as freely-suspended cells. In this work, the Spodoptera exigua Se301 cell line was used to produce the commercially available S. exigua nucleopolyhedrovirus (SeMNPV) in suspension. Se301 cells showed to be very sensitive to the hydrodynamic shear rates developed in bioreactors. A process of progressive adaptation to freely-suspended cultures using protective additives against shear stress and disaggregant was proposed. The best combinations were polyvinyl alcohol (PVA) or polyvinyl pyrrolidone (PVP) with the disaggregant dextran sulfate (DS). Both static and freely-suspended Se301 cell cultures were successfully infected with the SeMNPV baculovirus. Production of occluded baculovirus (OB) increased with the multiplicity of infection (MOI [ 0.1).

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Effects of Pluronic F-68 on Tetrahymena cells: protection against chemical and physical stress and prolongation of survival under toxic conditions

Cited by 28 publications

References 17 publications

Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics

Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics

In vitro investigation and biomechanical modeling of the effects of PLF-68 on osteoarthritis in a three-dimensional model

Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV)

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