Effects of polyaluminium chloride addition on community structures of polyphosphate and glycogen accumulating organisms in biological phosphorus removal (BPR) systems

Wang, Baogui; Zeng, Wei; Fan, Zhiwei; Wang, Chunyan; Meng, Qing; Peng, Yongzhen

doi:10.1016/j.biortech.2019.122431

Cited by 23 publications

(5 citation statements)

References 44 publications

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“…S8). Previous 16S rRNA gene-based studies have reported similar increases in Dechloromonas's abundance after treatment by polyaluminium chloride [78], cadmium [8], and copper [79] in sludge systems, indicating its remarkable resistance to metallic toxicants. Conversely, Ca.…”

Section: Discussionmentioning

confidence: 55%

Revealing taxon-specific heavy metal-resistance mechanisms in denitrifying phosphorus removal sludge using genome-centric metaproteomics

Lin

Wang

et al. 2021

Microbiome

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Background Denitrifying phosphorus removal sludge (DPRS) is widely adopted for nitrogen and phosphorus removal in wastewater treatment but faces threats from heavy metals. However, a lack of understanding of the taxon-specific heavy metal-resistance mechanisms hinders the targeted optimization of DPRS’s robustness in nutrient removal. Results We obtained 403 high- or medium-quality metagenome-assembled genomes from DPRS treated by elevating cadmium, nickel, and chromium pressure. Then, the proteomic responses of individual taxa under heavy metal pressures were characterized, with an emphasis on functions involving heavy metal resistance and maintenance of nutrient metabolism. When oxygen availability was constrained by high-concentration heavy metals, comammox Nitrospira overproduced highly oxygen-affinitive hemoglobin and electron-transporting cytochrome c-like proteins, underpinning its ability to enhance oxygen acquisition and utilization. In contrast, Nitrosomonas overexpressed ammonia monooxygenase and nitrite reductase to facilitate the partial nitrification and denitrification process for maintaining nitrogen removal. Comparisons between phosphorus-accumulating organisms (PAOs) demonstrated different heavy metal-resistance mechanisms adopted by Dechloromonas and Candidatus Accumulibacter, despite their high genomic similarities. In particular, Dechloromonas outcompeted the canonical PAO Candidatus Accumulibacter in synthesizing polyphosphate, a potential public good for heavy metal detoxification. The superiority of Dechloromonas in energy utilization, radical elimination, and damaged cell component repair also contributed to its dominance under heavy metal pressures. Moreover, the enrichment analysis revealed that functions involved in extracellular polymeric substance formation, siderophore activity, and heavy metal efflux were significantly overexpressed due to the related activities of specific taxa. Conclusions Our study demonstrates that heavy metal-resistance mechanisms within a multipartite community are highly heterogeneous between different taxa. These findings provide a fundamental understanding of how the heterogeneity of individual microorganisms contributes to the metabolic versatility and robustness of microbiomes inhabiting dynamic environments, which is vital for manipulating the adaptation of microbial assemblages under adverse environmental stimuli.

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Section: Discussionmentioning

confidence: 55%

Revealing taxon-specific heavy metal-resistance mechanisms in denitrifying phosphorus removal sludge using genome-centric metaproteomics

Lin

Wang

et al. 2021

Microbiome

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“…Moreover, Rezakhani et al [68] reported that Pseudomonas played a prominent role in the enhancement of plant uptake of phosphorous; however, the phosphorous content and concentration in the plant was lower in tank 2 than tank 1 (Table S2), even though Pseudomonas was most abundant in tank 2 during the experiment, suggesting the major role of the plant was to provide a root system for phosphorous adsorption instead of absorption, while Acinetobacter is known for simultaneously removing N and P by utilization of nitrate as an electron acceptor [69]. Candidatus Accumulibacter and Microlunatus that remove P through accumulation were apparently not affected by ENR [70][71][72][73], while Dechloromonas, a polyphosphate-accumulating organism (PAO), was absent from the ENR-treated tank 1 [74].…”

Section: Effect Of Enrofloxacin On Microbial Communitiesmentioning

confidence: 99%

Effects of Enrofloxacin on Nutrient Removal by a Floating Treatment Wetland Planted with Iris pseudacorus: Response and Resilience of Rhizosphere Microbial Communities

et al. 2022

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Constructed wetlands (CWs), including floating treatment wetlands (FTWs), possess great potential for treating excessive nutrients in surface waters, where, however, the ubiquitous presence of antibiotics, e.g., enrofloxacin (ENR), is threatening the performance of CWs. In developing a more efficient and resilient system, we explored the responses of the FTW to ENR, using tank 1, repeatedly exposed to ENR, and tank 2 as control. Plant growth and nutrient uptake were remarkably enhanced in tank 1, and similar phosphorus removal rates (86~89% of the total added P) were obtained for both tanks over the experimental period. Contrarily, ENR apparently inhibited N removal by tank 1 (35.1%), compared to 40.4% for tank 2. As ENR rapidly decreased by an average of 71.6% within a week after each addition, tank 1 took only 4 weeks to adapt and return to a similar state compared to that of tank 2. This might be because of the recovery of microbial communities, particularly denitrifying and antibiotic-resistance genes containing bacteria, such as Actinobacteria, Patescibacteria, Acidovorax and Pseudomonas. After three ENR exposures over six weeks, no significant differences in the nutrient removal and microbial communities were found between both tanks, suggesting the great resilience of the FTW to ENR.

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“…In general, excessive Fe(III) reacts chemically with phosphate (e.g., chemiprecipitation or chemisorption), resulting in a high C/P ratio within the system, which can promote GAOs proliferation and reduce phosphorus removal efficiency ( Welles et al, 2015 ). But existing studies have indicated that PAOs persist in AS systems even in the presence of high metal salt dosage ( Wang et al, 2020 ). As a result, it cannot be ruled out that PAOs indeed change their metabolic strategy in order to survive the Fe(III) stress.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of phosphorus removal performance in activated sludge by Fe(III) exposure: transitions in dominant metabolic pathways

Hong,

Cheng,

Huangfu

et al. 2024

Front. Microbiol.

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IntroductionSimultaneous chemical phosphorus removal process using iron salts (Fe(III)) has been widely utilized in wastewater treatment to meet increasingly stringent discharge standards. However, the inhibitory effect of Fe(III) on the biological phosphorus removal system remains a topic of debate, with its precise mechanism yet to be fully understood.MethodsBatch and long-term exposure experiments were conducted in six sequencing batch reactors (SBRs) operating for 155 days. Synthetic wastewater containing various Fe/P ratios (i.e., Fe/P = 1, 1.2, 1.5, 1.8, and 2) was slowly poured into the SBRs during the experimental period to assess the effects of acute and chronic Fe(III) exposure on polyphosphate-accumulating organism (PAO) growth and phosphorus metabolism.ResultsExperimental results revealed that prolonged Fe(III) exposure induced a transition in the dominant phosphorus removal mechanism within activated sludge, resulting in a diminished availability of phosphorus for bio-metabolism. In Fe(III)-treated groups, intracellular phosphorus storage ranged from 3.11 to 7.67 mg/g VSS, representing only 26.01 to 64.13% of the control. Although the abundance of widely reported PAOs (Candidatus Accumulibacter) was 30.15% in the experimental group, phosphorus release and uptake were strongly inhibited by high dosage of Fe(III). Furthermore, the abundance of functional genes associated with key enzymes in the glycogen metabolism pathway increased while those related to the polyphosphate metabolism pathway decreased under chronic Fe(III) stress.DiscussionThese findings collectively suggest that the energy generated from polyhydroxyalkanoates oxidation in PAOs primarily facilitated glycogen metabolism rather than promoting phosphorus uptake. Consequently, the dominant metabolic pathway of communities shifted from polyphosphate-accumulating metabolism to glycogen-accumulating metabolism as the major contributor to the decreased biological phosphorus removal performance.

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Effects of polyaluminium chloride addition on community structures of polyphosphate and glycogen accumulating organisms in biological phosphorus removal (BPR) systems

Cited by 23 publications

References 44 publications

Revealing taxon-specific heavy metal-resistance mechanisms in denitrifying phosphorus removal sludge using genome-centric metaproteomics

Revealing taxon-specific heavy metal-resistance mechanisms in denitrifying phosphorus removal sludge using genome-centric metaproteomics

Effects of Enrofloxacin on Nutrient Removal by a Floating Treatment Wetland Planted with Iris pseudacorus: Response and Resilience of Rhizosphere Microbial Communities

Inhibition of phosphorus removal performance in activated sludge by Fe(III) exposure: transitions in dominant metabolic pathways

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