Effects of polymer grafting on a glass surface for protein chip applications

Kim, Jaekwon; Shin, Dong‐Sik; Chung, Woo‐Jae; Jang, Kyung‐In; Lee, Kook-Nyung; Kim, Yong-Kweon; Lee, Jun Young

doi:10.1016/j.colsurfb.2003.08.015

Cited by 50 publications

(40 citation statements)

References 28 publications

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“…The fluorescent intensities of the amino functionalized glass substrates was measured after fluorescein isothiocyanate (FITC) had been coupled to the amino moieties on the surface to verify the covalent bonding between substrates and SAMs (Kim et al 2004). The results reveal that the normalized fluorescent intensity was between 0.22 and 1.…”

Section: Chemical Modification With Fluorescein Isothiocyanatementioning

confidence: 99%

Fabrication of protein chips based on 3-aminopropyltriethoxysilane as a monolayer

Jang

Liu

2008

Biomed Microdevices

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Although 3-aminopropyltriethoxysilane (APTES) is widely adopted as a monolayer in biosensors, experimental silanization takes at least 1 h at high temperature. Therefore, the feasibility of the silanization with APTES in a short reaction time and at room temperature was investigated. The surface modification of glass slides using a self-assembled monolayer of APTES with a concentration of 10% was studied by immobilizing FITC. APTES was successfully immobilized on the glass slide. The effect of reaction temperature and time of silanization were investigated. Various silanization conditions of APTES were examined by contact angle measurement and fluorescence microscopy. The surface of glass patterns with a gold thin film as background was characterized by determining the fluorescent intensities following the immobilization of fluorescein isothiocyanate (FITC), protein A-FITC, antimouse IgG-FITC and sheep anti-bovine albumin-FITC. The normalized fluorescent intensity indicated that a short period (4 min) of silanization at 25 degrees C suffices to form an APTES thin film by the immobilization of protein A on a glass surface. Such a condition does not require microheaters and temperature sensors in a microfluidic system, which will significantly reduce the manufacturing process, cost, and reaction time in the future.

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Section: Chemical Modification With Fluorescein Isothiocyanatementioning

confidence: 99%

Fabrication of protein chips based on 3-aminopropyltriethoxysilane as a monolayer

Jang

Liu

2008

Biomed Microdevices

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“…In general, the substrate modified by photo-polymerization shows a high RMS roughness compared with other polymer-modified substrates. 19,31 As shown in Figures 4VIII and 5VIII, the immobilized protein, the number of molecules and the relative S/N ratio were also lowest values at the singlemolecule level using the new detection system.…”

Section: Dual-color Tirfm System For Individual Protein Molecule Detementioning

confidence: 99%

“…An amine-coated substrate generally provides sufficient immine bridges with proteins, even though it causes steric hindrance between the protein molecules and surface due to the shortness of the arms of the amine coating materials. 19,31 The amine-modified substrate with many amine groups enhances the electrostatic interaction with the approaching proteins. The high intensity of the S/N ratio of Alexa Fluor ® 633-goat antirabbit IgG on the GPTS/CHI-modified chip substrate originated from the strong interaction between the protein molecules and substrate because the level of steric hindrance was decreased by the long arm length and the many amine groups on the modified-substrate surface.…”

Section: Methodsmentioning

confidence: 99%

“…The PL-modified substrate was then baked in an oven at 50 o C for 30 min. The APTS coating was carried out in a solution of 3-5% APTS in ethanol for 1 h. 17,19 The non-adsorbed APTS was removed by sonicating the APTS coating surfaces in ethanol for 10 min. The APTS-modified cover glasses were rinsed thoroughly with ethanol and blow dried with dry N 2 gas.…”

Section: Methodsmentioning

confidence: 99%

“…The GA and CHI coatings were coupled to the epoxy groups on the GPTS-modified substrates. 19 The GA coating was carried out using a 5% GA solution in 10 mM PBS at room temperature for 1 hour, rinsed in deionized water, and blow dried with N 2 gas. The CHI coating was carried out for 12 h at room temperature in a 1% CHI solution in deionized water, to which acetic acid was added until the solution became clear.…”

Section: Methodsmentioning

confidence: 99%

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Single-Protein Molecular Interactions on Polymer-Modified Glass Substrates for Nanoarray Chip Application Using Dual-Color TIRFM

Kim

Lee

Jung³

et al. 2007

Bulletin of the Korean Chemical Society

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The immobilization of proteins and their molecular interactions on various polymer-modified glass substrates [i.e. 3-aminopropyltriethoxysilane (APTS), 3-glycidoxypropyltrimethoxysilane (GPTS), poly (ethylene glycol) diacrylate (PEG-DA), chitosan (CHI), glutaraldehyde (GA), 3-(trichlorosilyl)propyl methacrylate (TPM), 3'-mercaptopropyltrimethoxysilane (MPTMS), glycidyl methacrylate (GMA) and poly-l-lysine (PL).] for potential applications in a nanoarray protein chip at the single-molecule level was evaluated using prismtype dual-color total internal reflection fluorescence microscopy (dual-color TIRFM). A dual-color TIRF microscope, which contained two individual laser beams and a single high-sensitivity camera, was used for the rapid and simultaneous dual-color detection of the interactions and colocalization of different proteins labeled with different fluorescent dyes such as Alexa Fluor ® 488, Qdot ® 525 and Alexa Fluor ® 633. Most of the polymer-modified glass substrates showed good stability and a relative high signal-to-noise (S/N) ratio over a 40-day period after making the substrates. The GPTS/CHI/GA-modified glass substrate showed a 13.5-56.3% higher relative S/N ratio than the other substrates. 1% Top-Block in 10 mM phosphate buffered saline (pH 7.4) showed a 99.2% increase in the blocking effect of non-specific adsorption. These results show that dual-color TIRFM is a powerful methodology for detecting proteins at the single-molecule level with potential applications in nanoarray chips or nano-biosensors.

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Chemical Patterning on Surfaces and in Bulk Gels

Karthaus

2017

Handbook of Solid State Chemistry

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Chemical patterns can be defined as patterns on a substrate that exhibit a chemical contrast, or as patterns that are formed by a chemical reaction, either on a surface or in bulk. This chapter classifies the surface pattern in further subgroups, depending on their formation technique (top‐down or bottom‐up), and gives some applications for surface patterns. The bulk patterns described here, Liesegang rings, are formed by a chemical reaction in diffusing electrolyte solutions in a gel. This technique to prepare a pattern is quite old, but still poorly understood, and their formation mechanism controversial.

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Effects of polymer grafting on a glass surface for protein chip applications

Cited by 50 publications

References 28 publications

Fabrication of protein chips based on 3-aminopropyltriethoxysilane as a monolayer

Fabrication of protein chips based on 3-aminopropyltriethoxysilane as a monolayer

Single-Protein Molecular Interactions on Polymer-Modified Glass Substrates for Nanoarray Chip Application Using Dual-Color TIRFM

Chemical Patterning on Surfaces and in Bulk Gels

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