Single-Protein Molecular Interactions on Polymer-Modified Glass Substrates for Nanoarray Chip Application Using Dual-Color TIRFM

Kim, Daekwang; Lee, Hee Gu; Jung, Hyungil; Kang, Seong Ho

doi:10.5012/bkcs.2007.28.5.783

Cited by 20 publications

(1 citation statement)

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“…A specific antibody (AB) was linked to the native oxide of the device by means of silane chemistry [26] to functionalize the SiNW, and blocking agents [27] were used to improve the surface specificity of the sensor. Both protocols for surface biofunctionalization and antigen (AG) sensing are described in detail in the experimental section of the SI.…”

Section: Methodsmentioning

confidence: 99%

Surface trap mediated electronic transport in biofunctionalized silicon nanowires

et al. 2016

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Silicon nanowires (SiNWs), fabricated via a top-down approach and then functionalized with biological probes, are used for electrically-based sensing of breast tumor markers. The SiNWs, featuring memristive-like behavior in bare conditions, show, in the presence of biomarkers, modified hysteresis and, more importantly, a voltage memory component, namely a voltage gap. The voltage gap is demonstrated to be a novel and powerful parameter of detection thanks to its high-resolution dependence on charges in proximity of the wire. This unique approach of sensing has never been studied and adopted before. Here, we propose a physical model of the surface electronic transport in Schottky barrier SiNW biosensors, aiming at reproducing and understanding the voltage gap based behavior. The implemented model describes well the experimental I-V characteristics of the device. It also links the modification of the voltage gap to the changing concentration of antigens by showing the decrease of this parameter in response to increasing concentrations of the molecules that are detected with femtomolar resolution in real human samples. Both experiments and simulations highlight the predominant role of the dynamic recombination of the nanowire surface states, with the incoming external charges from bio-species, in the appearance and modification of the voltage gap. Finally, thanks to its compactness, and strict correlation with the physics of the nanodevice, this model can be used to describe and predict the I-V characteristics in other nanostructured devices, for different than antibody-based sensing as well as electronic applications.

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Section: Methodsmentioning

confidence: 99%

Surface trap mediated electronic transport in biofunctionalized silicon nanowires

et al. 2016

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Nanoparticle Transport Through Polymers and Along Interfaces

Boyle

Maguire

Rose

et al. 2022

Macromolecular Engineering

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Transport of nanoparticles has received increasing attention for both fundamental interest to test prevailing models as well as practical interest in separations and self‐healing applications. Methods for measuring particle dynamics are briefly reviewed to orient the reader to different methodologies. The transport of nanoparticles through polymer melts, solutions, and cross‐linked networks is described with emphasis on systems where the nanoparticle is on the length scale of the defining structure including the tube size, correlation length, and mesh size, respectively. Because nanoparticles can interact with the different mediums, the interactions between nanoparticles and polymer and the subsequent impacts on dynamics are described. The transport of particles at liquid–solid (polymer brush) interfaces is addressed due to growing interest in applications ranging from understanding dynamics in porous media to the translocation of DNA. Both hard particles and polymer grafted particles (soft particles) are described. The objective of this article is to provide the reader with fundamental descriptions of phenomena while reviewing the current state of understanding. Further directions will leverage advances in inorganic chemistry to prepare monodisperse, functional nanoparticles as well as polymer chemistry to create novel media, such as networks with monodisperse mesh size.

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