Effects of pore size in 3-D fibrous matrix on human trophoblast tissue development

Abstract

The effects of pore size in a 3‐D polyethylene terephthalate (PET) nonwoven fibrous matrix on long‐term tissue development of human trophoblast ED27 cells were studied. Thermal compression was used to modify the porosity and pore size of the PET matrix. The pore size distributions in PET matrices were quantified using a liquid extrusion method. Cell metabolic activities, estradiol production, and cell proliferation and differentiation were studied for ED27 cells cultured in the thermally compressed PET matrices with known pore structure characteristics. In general, metabolic activities and proliferation rate were higher initially for cultures grown in the low‐porosity (LP) PET matrix (porosity of 0.849, average pore size of 30 μm in diameter) than those in the high‐porosity (HP) matrix (porosity of 0.896, average pore size of 39 μm in diameter). However, 17β‐estradiol production and cell differentiation activity in the HP matrix surpassed those in the LP matrix after 12 days. The expression levels of cyclin B1 and p27kip1 in cells revealed progressively decreasing proliferation and increasing differentiation activities for cells grown in PET matrices. Also, difference in pore size controlled the cell spatial organization in the PET matrices and contributed to the tissue development in varying degrees of proliferation and differentiation. It was also found that cells grown on the 2‐D surface behaved differently in cell cycle progression and did not show increased differentiation activities after growth had stopped and proliferation activities had lowered to a minimal level. The results from this study suggest that the 3‐D cell organization guided by the tissue scaffold is important to tissue formation in vitro. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 606–618, 2000.

Three-Dimensional Scaffolds for Tissue Engineering Applications: Role of Porosity and Pore Size

“…Ma et al fabricated 3D polyethylene terephthalate (PET) nonwoven fibrous matrix and modified the pore size and porosity by using thermal compression. 132 Two types of matrices were studied, namely low porosity (LP) and high porosity (HP). The LP matrices had a porosity of 0.849 and an average pore size of 30 mm, while HP matrices had a porosity of 0.896 and an average pore size of 39 mm.…”

Modeling Tissue Growth Within Nonwoven Scaffolds Pores

“…They aid the transportation of nutrients, and waste, in and out of the scaffold, 1 cell migration through the scaffold, 2 and the formation of a coherent tissue as a result of pore infiltration by cells. 3 Fiber-based scaffolds are commonly used and can be manufactured by, for example, nonwoven, [4][5][6] electrospinning, 7,8 or fused fiber deposition [9][10][11] technologies. Within these scaffolds voids between adjacent fibers form pores, with fiber segments representing the pore struts, and crossing fibers, the strut intersections.…”

Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

“…A number of studies have been done to reveal the effects of 2D versus 3D cell culture on differentiation, [21][22][23][24][25] drug metabolism, [26][27][28][29][30] gene expression and protein synthesis, [31][32][33][34][35] general cell function, [36][37][38][39][40] increase of in vivo relevance, 31,[41][42][43] morphology, [44][45][46][47] proliferation, 26,[48][49][50][51] response to stimuli, 41,[52][53][54] viability, 36,[55][56][57] and migration. [58][59][60][61] Adding the third dimension to cellular environment provides them with more in vivo-like morphology, behaviors, and intercellular interactions for understanding cytology in more physiological conditions.…”