Effects of probiotic supplementation on serum trimethylamine-N-oxide level and gut microbiota composition in young males: a double-blinded randomized controlled trial

Chen, Si; Jiang, Pingping; Yu, Danxia; Liao, Gong‐Cheng; Wu, Shangling; Fang, Aiping; Chen, Peiyan; Wang, Xiaoyan; Luo, Yun; Long, Jing‐An; Zhong, Renxin; Liu, Zhao-Yan; Li, Chunlei; Zhang, Daoming; Zhu, Huilian

doi:10.1007/s00394-020-02278-1

Cited by 31 publications

(21 citation statements)

References 45 publications

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“…plantarum ZDY04, and Lacticaseibacillus paracasei can reduce TMAO and TMA levels by modifying the gut microbiota [ 41 , 42 , 43 ]. In contrast, in human clinical trials, supplementation of single or multistrain probiotics serum TMAO levels was not reduced [ 10 , 11 , 13 , 44 , 45 ]. New potential probiotic strains are needed to reduce TMAO levels in vivo and in humans.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Identification and Selection of Probiotic Strains Able to Reduce the Serum TMAO Level in Mice Challenged with Choline

Ramireddy

Tsen

Chiang

et al. 2021

Foods

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Trimethylamine oxide (TMAO) originates from trimethylamine (TMA), which is oxidized in the liver by hepatic flavin-containing monooxygenases (FMO3). TMA is produced by its dietary precursors such as choline, carnitine, and phosphatidylcholine by gut microbiota. TMAO attracts attention, identified as a novel and independent risk factor for promoting obesity, atherosclerosis and cardiovascular disease (CVD), chronic kidney disease (CKD), insulin tolerance, and colon cancer. Probiotics have been considered as live microorganisms, providing benefits to their host when they are given in sufficient quantities and administered continuously. The objective of this study is to suggest a method to select potential probiotic strains to reduce the serum concentration of TMAO in mice fed with choline. In this work, we chose three lactobacilli with strong adherence capability, and fed multistrain formula (MF) to the mice challenged with choline. On days 7, 14, and day 28, it was found that the MF-containing L. amylovorus LAM1345, Lpb. plantarum LP1145, and Lim. fermentum LF33 showed a significant reduction in serum TMAO and TMA levels. For the single strains, LP1145 reduced TMAO on days 14 and 28, and strain LAM1345 reduced TMAO significantly on days 7 and day 14. For strain LF1143 from strain LF33, it showed no significant effect on TMAO and TMA. Thus, MF showed the best effect, which may be due to the additive and synergetic effect and the contribution of strain LP1145 and LAM1345. Finally, for the LAM1345 and LP1145 strains, we used molecular identification and typing methods to assure that these two strains are unique strains. The methods used for LAM 1345 were leader peptidase A (lepA) gene analysis and phylogenetic analysis, while for strain LP 1145and other strains of Lpb. plantarum subsp. plantarum sequences were compared using the whole-genome multilocus sequence typing (wgMLST) method.

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Section: Discussionmentioning

confidence: 99%

“…Another proposed therapeutic approach is the use of probiotics potentially effective in altering the microbiota composition. Numerous studies suggested that the use of probiotics, single strain, or multistrain could influence the intestinal microbiome composition but failed to reduce serum TMAO levels [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Selection of Probiotic Strains Able to Reduce the Serum TMAO Level in Mice Challenged with Choline

Ramireddy

Tsen

Chiang

et al. 2021

Foods

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“…Boutagy et al tried decreasing TMAO levels in the intestinal flora using probiotic supplements but noted that adding the multi-strain probiotic VSL#3 failed to alter plasma TMAO levels and did not affect the plasma concentration of L-carnitine, choline, or betaine ( 76 ). In another research, a higher proportion of participants in the probiotic group had reduced TMAO after probiotic intervention ( 77 ). Different probiotics have different abilities to metabolize choline, which may lead to contradictory research results.…”

Section: Preventions and Treatments Targeting Tmaomentioning

confidence: 97%

Targeting Trimethylamine N-Oxide: A New Therapeutic Strategy for Alleviating Atherosclerosis

Jing

Zhang

Xiang

et al. 2022

Front. Cardiovasc. Med.

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Atherosclerosis (AS) is one of the most common cardiovascular diseases (CVDs), and there is currently no effective drug to reverse its pathogenesis. Trimethylamine N-oxide (TMAO) is a metabolite of the gut flora with the potential to act as a new risk factor for CVD. Many studies have shown that TMAO is involved in the occurrence and development of atherosclerotic diseases through various mechanisms; however, the targeted therapy for TMAO remains controversial. This article summarizes the vital progress made in relation to evaluations on TMAO and AS in recent years and highlights novel probable approaches for the prevention and treatment of AS.

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“…Chen et al [ 27 ] analyzed the fecal microbiota of 20 healthy men (aged 20 to 25 years) of normal body mass index (BMI) by 16S rRNA gene sequencing after 4 weeks of probiotic intake (2 g containing 1.32 × 10 11 CFUs live bacteria, including strains of Lactobacillus acidophilus , Lactobacillus rhamnosus GG, Bifidobacterium animalis , and Bifidobacterium longum ). They reported a significant increase in the relative abundances of Faecalibacterium prausnitzii ( p = 0.043) and Prevotella ( p = 0.001) compared with those in 20 healthy subjects in the placebo intake group, but there were no obvious differences in α- and β-diversity.…”

Section: Effect On Gut Microbiotamentioning

confidence: 99%

“…They reported a significant increase in the relative abundances of Faecalibacterium prausnitzii ( p = 0.043) and Prevotella ( p = 0.001) compared with those in 20 healthy subjects in the placebo intake group, but there were no obvious differences in α- and β-diversity. Chen et al [ 27 ] also measured serum trimethylamine- N -oxide and its precursors, which may contribute to the risk of developing atherosclerosis and cardiovascular disease [ 28 ], but they did not detect any attenuation of these parameters associated with detectable changes in the fecal microbiota upon probiotic intake.…”

Section: Effect On Gut Microbiotamentioning

confidence: 99%

Probiotics: A Dietary Factor to Modulate the Gut Microbiome, Host Immune System, and Gut–Brain Interaction

2020

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Various benefits of probiotics to the host have been shown in numerous human clinical trials. These organisms have been proposed to act by improving the balance of the gut microbiota and enhancing the production of short-chain fatty acids, as well as by interacting with host cells in the gastrointestinal tract, including immune cells, nerve cells, and endocrine cells. Although the stimulation of host cells by probiotics and subsequent signaling have been explained by in vitro experiments and animal studies, there has been some skepticism as to whether probiotics can actually interact with host cells in the human gastrointestinal tract, where miscellaneous indigenous bacteria coexist. Most recently, it has been shown that the ileal microbiota in humans after consumption of a fermented milk is occupied by probiotics for several hours, indicating that there is adequate opportunity for the ingested strain to stimulate the host cells continuously over a period of time. As the dynamics of ingested probiotics in the human gastrointestinal tract become clearer, further progress in this research area is expected to elucidate their behavior within the tract, as well as the mechanism of their physiological effects on the host.

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Effects of probiotic supplementation on serum trimethylamine-N-oxide level and gut microbiota composition in young males: a double-blinded randomized controlled trial

Cited by 31 publications

References 45 publications

Molecular Identification and Selection of Probiotic Strains Able to Reduce the Serum TMAO Level in Mice Challenged with Choline

Molecular Identification and Selection of Probiotic Strains Able to Reduce the Serum TMAO Level in Mice Challenged with Choline

Targeting Trimethylamine N-Oxide: A New Therapeutic Strategy for Alleviating Atherosclerosis

Probiotics: A Dietary Factor to Modulate the Gut Microbiome, Host Immune System, and Gut–Brain Interaction

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