Effects of prolactin on aldosterone secretion in rat zona glomerulosa cells

Abstract

Acute effects and action mechanisms of prolactin (PRL) on aldosterone secretion in zona glomerulosa (ZG) cells were investigated in ovariectomized rats. Administration of ovine PRL (oPRL) increased aldosterone secretion in a dose-dependent manner. Incubation of [3H]-pregnenolone combined with oPRL increased the production of [3H]-aldosterone and [3H]-deoxycorticosterone but decreased the accumulation of [3H]-corticosterone. Administration of oPRL produced a marked increase of adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in ZG cells. The stimulatory effect of oPRL on aldosterone secretion was attenuated by the administration of angiotensin II (Ang II) and high potassium. The Ca2+ chelator, ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA, 10(-2) M), inhibited the basal release of aldosterone and completely suppressed the stimulatory effects of oPRL on aldosterone secretion. The stimulatory effects of oPRL on aldosterone secretion were attenuated by the administration of nifedipine (L-type Ca2+ channel blocker) and tetrandrine (T-type Ca2+ channel blocker). These data suggest that the increase of aldosterone secretion by oPRL is in part due to (1) the increase of cAMP production, (2) the activation of both L- and T-type Ca2+ channels, and (3) the activation of 21-hydroxylase and aldosterone synthase in rat ZG cells.

Prolactin induces phosphorylation of the STAT5 in adrenal glands of Hatano rats during stress

“…Previous studies have shown that PRL receptors (PRLR) are expressed in adrenal cortex in several species (McDonough and Ewig 1982;Klemcke et al 1989;Ouhtit et al 1993;Glasow et al 1996;Glasow et al 1998). In addition to its regulatory function in the reproductive system (such as in lactation), PRL has been shown to stimulate corticosterone secretion by primary rat adrenocortical cell cultures in-vitro (Lo et al 1998;Chang et al 1999;Kau et al 1999;Lo and Wang 2002;Kan et al 2003;Lo et al 2006;Jaroenporn et al 2007). These observations led us to postulate that stress-induced PRL elevation may enhance the secretion of corticosterone in response to stress.…”

Renal effects of recombinant prolactin in anaesthetized rats

“…Furthermore, prolactin administration has been associated with an increased aldosterone secretion by rat zona glomerulosa cells (35). These findings are suggestive of a possible influence on aldosterone release with a consequent salt-retaining effect, possibly resulting in a vasopressin-independent increase in water reabsorption.…”

Direct Effects of Prolactin on Adrenal Steroid Release in Male Hatano High-Avoidance (HAA) Rats May be Mediated Through Janus Kinase 2 (Jak2) Activity

“…Similar effects have been found for PRL o n s e c r e t i o n o f a l d o s t e r o n e , c o r t i s o l , d e h y d r o e p i a n d r o s t e r o n e ( D H E A ) a n d / o r dehydroepiandrosterone sulfate (DHEAS) in human and nonhuman primate adrenals [11, 19- 22]. Moreover, PRL increase in vitro secretion of corticosterone and aldosterone in rat adrenocortical cells [4,[23][24][25][26][27]. In the present study, PRL stimulation of adrenal steroid release was found to be dependent on Jak2 activity since PRL-induced adrenal steroid release was significantly prevented by AG490.…”