Effects of prolactin on intracellular stored calcium in the course of bovine oocyte maturation in vitro

Kuzmina, T. I.; Lebedeva, I.; Torner, H.; Alm, H.; Denisenko, V. Yu.

doi:10.1016/s0093-691x(99)00080-1

Cited by 10 publications

(8 citation statements)

References 25 publications

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“…To evaluate nuclear maturation and the M-II chromosome morphology in oocytes, cytogenetic preparations were performed by the method of Tarkowski (1966) with some modifications ( Kuzmina et al, 1999 ). The state of the nuclear material was examined under a light microscope (Opton, Germany) at magnification × 1000 using criteria described earlier ( Ernst et al, 1980 ; Homa, 1988 ).…”

Section: Methodsmentioning

confidence: 99%

“…Receptors of PRL and GH or their mRNA have been detected in oocytes and surrounding cumulus cells of different species including cows ( Bevers and Izadyar, 2002 ; Marchal et al, 2003 ; Picazo et al, 2004 ; Lebedeva et al, 2014b ). Both hormones can modulate the mitochondrial activity and/or calcium homeostasis in bovine oocytes maturing in vitro ( Kuzmina et al, 1999 , 2007 ), indicating the hormonal implication in processes modified by aging. Furthermore, in various types of mammalian cells, PRL and GH are able to activate signal cascades dependent on MAP kinase, protein kinase C, and Akt ( Postel-Vinay and Finidori, 1995 ; Bole-Feysot et al, 1998 ; Di Rosa et al, 2009 ; Devesa et al, 2014 ), which in turn are involved in regulation of some functional changes in aging oocytes.…”

Section: Introductionmentioning

confidence: 99%

“…We have previously found abnormal modifications of the chromosome morphology in bovine M-II oocytes aging in vitro , with the surrounding cumulus investment promoting these negative processes ( Lebedeva et al, 2014a ). Since PRL and GH at certain concentrations could exert inhibitory effects mediated by cumulus/granulosa cells on destructive changes of chromosomes in bovine oocytes maturing in vitro ( Kuzmina et al, 1999 ; Lebedeva et al, 2005 ), one would expect similar hormonal effects on the senescent oocytes. Therefore, the present study was conducted to test a hypothesis that PRL and GH are able to suppress the M-II chromosome aberrations in aging bovine oocytes by transmitting signals through somatic cumulus cells.…”

Section: Introductionmentioning

confidence: 99%

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Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

et al. 2015

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General senescence of the adult organism is closely connected with reproductive one. Meanwhile, the age-related reduction in the female fertility is primarily associated with a decline in the gamete quality. Molecular and cellular changes in oocytes of old mammalian females are very similar to those occurring during aging of matured ova of their young counterparts, suggesting similarities in underlying mechanisms. The aim of the present work was to study actions of two related pituitary hormones, prolactin (PRL) and growth hormone (GH), on age-associated modifications of metaphase-II (M-II) chromosomes in bovine oocytes using a model of the prolonged culture. We analyzed: (1) effects of PRL and GH on abnormal changes in the chromosome morphology in aging matured oocytes and the role of cumulus cells in these effects and (2) signaling pathways involved in the hormone actions. During the prolonged culture of oocytes, a gradual rise in the frequency of destructive modifications of M-II chromosomes was revealed. In the case of cumulus-enclosed oocytes (CEOs), PRL and GH exerted dose-dependent biphasic effects on the frequency of these modifications. Both PRL (50 ng/ml) and GH (10 ng/ml) decelerated the abnormal chromosome changes in CEOs, but did not affect the chromosome configuration in denuded oocytes. Concurrently, the presence of PRL and GH receptors in cumulus cells surrounding matured oocytes was demonstrated. Attenuating effects of both hormones on the chromosome modifications in aging CEOs were abolished by PP2 (an inhibitor of Src-family tyrosine kinases), triciribine (an inhibitor of Akt kinase), and calphostin C (a protein kinase C inhibitor). Our findings indicate that PRL and GH can exert the similar decelerating action on age-associated alterations in the M-II chromosome morphology in bovine ova, which is mediated by cumulus cells and may be related to activation of Src-family tyrosine kinases as well as Akt- and protein kinase C-dependent signal pathways.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

et al. 2015

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“…The levels of [Ca 2+ ] is in the oocytes were estimated on the basis of previously described measurements o f t h e f l u o r e s c e n c e i n t en s i t y o f c a l c i u mchlortetracycline (CTC) complexes on irradiation [17,18]. The fluorescence intensity was recorded under a luminescent microscope (LUMAM-I-1, LOMO, St. Petersburg, Russia) equipped with a special photoelectronic multiplier (FEU-39A), an excitation filter (FS1; 6-mm thick), a green analysis filter, an interference light filter (passing maximum wavelength of 520 nm), and a mercury lamp.…”

Section: Intracellular Stored Calcium [Ca 2+ ] Is Assaymentioning

confidence: 99%

Effect of Recombinant Bovine Somatotropin (rbST) on Cytoplasmic Maturation of Bovine Oocytes and Their Developmental Competence In Vitro

Kuzmina

Alm

Denisenko

et al. 2007

Journal of Reproduction and Development

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Abstract. The objectives of this study were to evaluate the effects of recombinant bovine somatotropin (rbST) on the nuclear and cytoplasmic maturation of bovine oocytes and their further developmental competence to blastocysts in vitro. We analyzed the mitochondrial activity and concentration of intracellular stored calcium ([Ca 2+ ]is) in matured oocytes and the morphology and chromatin status of produced embryos after in vitro fertilization. Cumulus-oocyte complexes were incubated in TCM 199 containing 10% fetal calf serum (control medium 1: CM 1) or 10% estrus cow serum (control medium 2: CM 2). The culture medium of the treatment groups was modified by supplementation of the control medium with 10 ng/ml rbST (CM 1A and CM 2A), 10 6 /ml granulosa cells (CM 1B and CM 2B), or 10 ng/ml rbST plus 10 6 /ml granulosa cells (CM 1C and CM 2C). No differences were observed in the percentages of oocytes reaching metaphase II between the groups. However, the proportion of blastocysts was highest in treatment groups CM 1C and CM 2C (P<0.05). The type of serum did not alter the positive effect of rbST on the developmental competence of embryos. The fluorescence intensity of metabolically active mitochondria measured by intensity per oocyte (Em 570) after MitoTracker CMTM Ros Orange labeling was significantly increased in oocytes matured in the presence of 10 ng/ml rbST and granulosa cells (309.21 vs. 119.97 µA; P<0.01). In parallel, the concentration of [Ca 2+ ]is in oocytes, determined using fluorophore chlortetracycline, was significantly decreased (0.85 ± 0.02 vs. 0.97 ± 0.03 AU; P<0.05). Based on these results, we concluded that rbST, in interaction with granulosa cells stimulates the oxidative activity of ooplasmic mitochondria and decreases the content of [Ca 2+ ]is in oocytes. These facts support the hypothesis that somatotropin influences the developmental competence of bovine oocytes during maturation in vitro, and this effect can be modulated by granulosa cells.

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“…To assess the SPA of aged bovine oocytes, cytogenetic preparations were performed via the method of Tarkowski [ 55 ] with some modifications [ 56 ]. The state of the nuclear material in oocytes and presumable parthenotes was examined under a light microscope (Axio Scope.A1; Carl Zeiss, Oberkochen, Germany) at magnification ×1000 using criteria described earlier [ 57 ].…”

Section: Methodsmentioning

confidence: 99%

Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro

et al. 2021

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Aging processes accelerate dramatically in oocytes that have reached the metaphase-II (M-II) stage. The present work aimed to study the patterns and intracellular pathways of actions of prolactin (PRL) and growth hormone (GH) on age-associated changes in bovine M-II oocytes aging in vitro. To this end, we analyzed spontaneous parthenogenetic activation (cytogenetic assay), apoptosis (TUNEL assay), and the developmental capacity (IVF/IVC) of in vitro-matured oocytes after prolonged culturing. Both PRL and GH reduced the activation rate of aging cumulus-enclosed oocytes (CEOs) and denuded oocytes (DOs), and their respective hormone receptors were revealed in the ova. The inhibitor of Src-family tyrosine kinases PP2 eliminated the effects of PRL and GH on meiotic arrest in DOs, whereas the MEK inhibitor U0126 only abolished the PRL effect. Furthermore, PRL was able to maintain the apoptosis resistance and developmental competence of aging CEOs. The protein kinase C inhibitor calphostin C suppressed both the actions of PRL. Thus, PRL and GH can directly support meiotic arrest in aging M-II oocytes by activating MAP kinases and/or Src-family kinases. The effect of PRL in maintaining the developmental capacity of aging oocytes is cumulus-dependent and related to the pro-survival action of the protein kinase C-mediated signal pathway.

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Effects of prolactin on intracellular stored calcium in the course of bovine oocyte maturation in vitro

Cited by 10 publications

References 25 publications

Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways

Effect of Recombinant Bovine Somatotropin (rbST) on Cytoplasmic Maturation of Bovine Oocytes and Their Developmental Competence In Vitro

Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro

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