Effects of Prolonged Room Temperature Holding of Whole Blood Intended for Preparation of Components

Platelet concentrates (PCs) were prepared from single buffy coats derived from fresh blood and from blood units stored overnight, as well as from buffy coats that were stored overnight. The platelet yield from overnight-stored buffy coats was similar to that of fresh blood or overnight-stored blood. PCs were stored at 20-24°C and on day 5 of storage, platelet aggregation with ADP was tested both at 37 and 25°C. Stored platelets aggregated better at 25°C than 37°C. The maximal aggregation (10 µA/ ADP) of stored platelets from overnight-stored buffy coats was 46±23% (n = 30), while that of stored platelets prepared either from fresh or overnight-stored blood was 27±21 % (n = 29) and 22± 15% (n = 29), respectively. Extracellular lactate dehydrogenase and ammonia levels, as well as elastase activity were similar in stored PCs of different origin. Our conclusion is that PCs prepared from overnight-stored buffy coat might also be suitable for storage and clinical use. In vivo studies are needed to confirm our findings.

Section: Resultsmentioning

confidence: 92%

Storage of Platelet Concentrates from Overnight-Stored Blood and Overnight-Stored Buffy Coat: In vitro Studies

Ràcz¹,

Baróti²

1995

“…No difference in the in vivo recovery and life span of platelets was found when platelets were separated immediately or after a 6-or 8-hour delay [4,5,7], Avoy et al [7] stated that it would not be expected that extending the preparation period of platelets would have any demonstrable effect on platelet function and viability. As might also be expected, the platelet yield was unaf fected.…”

Section: Discussionmentioning

confidence: 99%

“…Current FDA regulations require that platelet con centrates shall be separated within 4 h after collection of the unit of whole blood or plasma [1], This period was increased to 6 h with the 9th edition of the AABB stan dards [2], Regional blood centers using mobile collection teams have logistic problems preparing platelet concen trates within this time. Several in vitro and in vivo studies have shown that an 8-hour delay in the processing of blood did not adversely affect platelets preserved in CPD, CPDA-1 or CPDA-2 [3][4][5]. It has also been shown that human red blood cells tolerate exposure to room temper ature for as long as 8 h without greater loss of ATP or viability than in cells refrigerated within 4 h of phleboto my [6][7][8][9].…”

Section: Methodsmentioning

confidence: 99%

In vitro Effect on Stored Red Blood Cells and Platelets after a 15-Hour Delayed Refrigeration of Whole Blood prior to Component Preparation in CPD-AD

Koerner¹,

Sahlmen²,

Stampe³

1986

We extended the time of keeping whole blood at 20-24 °C to 15 h (overnight) after phlebotomy for preparing platelet concentrates. We have evaluated the in vitro characteristics of platelets and blood cells prepared from whole blood drawn into CPD-AD, an anticoagulant containing 0.4 mM adenine and 1.5 times more dextrose than CPD. We studied in vitro red cell and platelet function of blood cooled either within 4 h after collection or after a 15-hour delay. In vitro platelet function measured as hypotonic shock reaction, aggregation response to ADP and collagen and 14C-serotonin uptake were not significantly different after preparation and after a 5-day storage period. Units held at room temperature for 15 h after blood collection exhibited a level of 2,3-DPG that was 45% of that exhibited by red cells held for 15 h at 1-6°C. All other in vitro parameters of red cell concentrates measured during 35 days of storage were not significantly different. Based on these in vitro data blood drawn into CPD-AD might be kept up to 15 h at room temperature prior to refrigeration in order to prepare platelet concentrates.

“…Other workers have already demonstrated that red cell and platelet preparations may be prepared up to 8 [6,[9][10][11][12][13][14][15] or 15 h [ 16] after collection of blood in CPD anti coagulants. The in vitro data presented here indicate that red cell and platelet preparations of good quality may be prepared 18 h after collection in 0.5 CPD.A2.…”

Section: Red Cell Preparationsmentioning

confidence: 99%

Studies on the Procurement of Blood Coagulation Factor VIII

Griffin¹,

Bell²,

Prowse³

1988

Our previous studies have shown that the use of half-strength citrate anticoagulant improves plasma factor VIII stability while maintaining the viability of red cells and platelets. This study extends these observations to show that the stability of factor VIII is maintained in blood stored at room temperature or 4°C overnight and that in vitro tests indicate comparable quality of red cells and platelets prepared from half-strength citrate donations processed immediately or after overnight storage at room temperature. The exception to this was a more rapid loss of red cell 2,3-diphosphoglycerate, as has previously been observed for full-strength citrate donations.