Effects of psychoactive drugs on delta sleep-inducing peptide concentrations in rat brain

Wahlestedt, Claes; Ekman, Rolf; Heilig, Markus; Widerlöv, Erik

doi:10.1016/0014-2999(89)90159-3

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Recently, a mild increase in the concentration of IR-DSIP in hypothalamic extracts 2 weeks after adrenalec tomy was described [35]. Different surgical methods were used in that study which makes it difficult to com pare the data with those obtained in the present study.…”

Section: Discussioncontrasting

confidence: 49%

Immunoreactive Delta Sleep-Inducing Peptide in the Rat Hypothalamus, Pituitary and Adrenal Gland: Effects of Adrenalectomy

Bjartell

Sundler²,

Ekman

1991

Horm Res

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Immunoreactive (IR) delta sleep-inducing peptide (DSIP) was examined by immunocytochemistry in the rat pituitary and adrenal gland and found to be colocalized with IR thyroid-stimulating hormone in the pituitary and with noradrenaline in the adrenal medulla. IR-DSIP was also detectable in nerve fibers in the posterior pituitary. By radioimmunoassay, IR-DSIP was quantified in plasma and tissue extracts after uni- or bilateral adrenalectomy. Significantly elevated plasma levels of IR-DSIP were measured 5 days after bilateral adrenalectomy (p < 0.001). IR-DSIP was increased (p < 0.05) in pituitary extracts from bilaterally adrenalectomized rats after 5 days, but not after 14 or 28 days. Sham- and unoperated animals did not significantly differ in plasma or tissue concentration of IR-DSIP. High-performance liquid chromatography of C₁₈ SEP-PAK® purified hypothalamus extracts revealed a single peak of IR-DSIP material of lower hydrophobicity than synthetic DSIP. The elevated concentration of IR-DSIP in the rat pituitary and plasma after bilateral adrenalectomy is consistent with the previously suggested role of DSIP to influence the activity of the hypothalamic-pituitary-adrenal axis.

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Section: Discussioncontrasting

confidence: 49%

Immunoreactive Delta Sleep-Inducing Peptide in the Rat Hypothalamus, Pituitary and Adrenal Gland: Effects of Adrenalectomy

Bjartell

Sundler²,

Ekman

1991

Horm Res

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Pharmacology of the CNS Peptides

Inoue¹

1995

Handbook of Experimental Pharmacology

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Delta-sleep-inducing peptide and its analogs and the serotoninergic system in the development of anticonvulsive influences

Шандра

Godlevskii

Brusentsov

et al. 1998

Neurosci Behav Physiol

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Experiments on rats were carried out to study the effects of administration of delta-sleep-inducing peptide (DSIP) and its analogs (9-14) into the reticular part of the substantia nigra and ventral hippocampus on picrotoxin- and kainate-induced epileptic activity. Additionally, the uptake of [3H]tryptophan by brain structures was studied. Intranigral and intrahippocampal microinjections of peptide and its analogs were found to have anticonvulsant effects against both picrotoxin- and kainate-induced epileptic activity. Studies of the effects of DSIP and its structural analogs on the uptake of tryptophan by brain structures showed that peptides predominantly increased uptake of this amino acid. It is suggested that brain structures which modulate tryptophan uptake are largely responsible for the anticonvulsant actions of DSIP and its analogs. The results obtained here provide evidence that the serotoninergic system is not of key importance in mediating the anticonvulsant effects of DSIP and its analogs.

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Effects of psychoactive drugs on delta sleep-inducing peptide concentrations in rat brain

Cited by 3 publications

References 18 publications

Immunoreactive Delta Sleep-Inducing Peptide in the Rat Hypothalamus, Pituitary and Adrenal Gland: Effects of Adrenalectomy

Immunoreactive Delta Sleep-Inducing Peptide in the Rat Hypothalamus, Pituitary and Adrenal Gland: Effects of Adrenalectomy

Pharmacology of the CNS Peptides

Delta-sleep-inducing peptide and its analogs and the serotoninergic system in the development of anticonvulsive influences

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