Effects of Puffer (Sphoeroides rubripes) Supplementation on Disruption of Antioxidant Defense Systems in Ethanol-Treated Rats

Abstract

SummaryWe investigated the effects of puffer ( Sphoeroides rubripes ) supplementation on antioxidant metabolism in ethanol-treated rats. Sprague-Dawley rats were randomly assigned into 4 groups of 7 rats each and fed (1) an AIN-93G diet (NC), (2) 25% ethanol (E), (3) 25% ethanol and an AIN-93G diet containing 1% puffer flesh (E ϩ F), or (4) 25% ethanol and an AIN-93G diet containing 1% puffer skin (E ϩ S) for 5 wk. At the end of the experimental period, the rats were sacrificed and their blood and organs were collected. To evaluate the effect of puffer supplementation, lipid-soluble antioxidant vitamin and conjugated diene (CD) levels, DNA damage, and mRNA expression of heme oxygenase-1 (HO-1) were assessed. Animals that were fed ethanol showed reduced plasma levels of lipid-soluble antioxidant vitamin and significantly increased levels of lipid peroxides, DNA damage, and HO-1 expression. Dietary supplementation with puffer conferred an antioxidant effect by significantly increasing the levels of ␥ -tocopherol, a lipid-soluble antioxidant vitamin, and by significantly decreasing the plasma levels of CD, DNA damage, and HO-1 expression. These results suggest that consumption of puffer improves the antioxidant status of ethanoltreated rats.

Protective effects of Opuntia humifusa cladode extracts against biostir AD-induced atopic dermatitis in NC/Nga mice

“…Each experimental group included 5 mice. The normal group was fed a diet consisting of AIN 93G purified rodent chow (13). The skin of mice in the control, OHW, and OHE groups was treated for 3 weeks with the natural mite inducer Biostir AD (Biostir Inc., Kobe, Japan) in order to induce atopic dermatitis.…”