Effects of resveratrol and methoxyamine on the radiosensitivity of iododeoxyuridine in U87MG glioblastoma cell line

Khoei, Samideh; Shoja, M R; Mostaar, Ahmad; Faeghi, Fariborz

doi:10.1177/1535370215622583

Cited by 14 publications

(16 citation statements)

References 26 publications

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“…Furthermore, RSV extremely blocked HIF-1 a protein secretion by altering both protein translation as well as HIF1-α protein mortification (39). It is therefore confirmed that RSV to augmented the IUdR absorption in hypoxia cells (64).…”

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 67%

“…Iododeoxyuridine (IUdR) is halogenated pyrimidines analogues. It is one the most effective non-hypoxic radiation sensitizers (64,119). It is a thymidine analogue so it able to insert itself into DNA during the cell cycling.…”

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

“…The quantity of radio sensitization directly reflected the amount of thymidine substitution during DNA replication (158). Studies has shown that the absorption of IUdR declines in spheroid culture (64). It is proven that hypoxia, a conjoint phenomenon in gliomas leads to a decrease in the sensitivity of glioma cells to radiation as well as chemotherapy.…”

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

“…This phenomenon is triggered via the hypoxia inducible factor-1 alpha (HIF1-α) pathway (122). Studies have shown that HIF1-α often translocate from cytoplasm to nucleus under hypoxia environments resulting in its binding to HIF1-β to forms the HIF1 complex (64,122). It stimulates about 60 diverse functional genes resulting in the augmentation of oxygen delivery.…”

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

“…Therefore, blockade of HIF1-α results in sensitization of resistance in glioma cells to the cytotoxic consequence of Radiation as well as chemotherapy. Studies have proven that RSV blocks HIF1-α protein release during hypoxia circumstance (64,122). A conformational study indicated that RVS's blockade effect results in significantly build-up hypoxia-induced HIF1-α protein in cancer cells but did not alter HIF1-α mRNA concentrations (122,169).…”

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

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The Therapeutic Potential of Resveratrol in Gliomas

Richard¹

2019

ABCMed

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Resveratrol (RSV) is found in most human foods especially fruits such as grapes, peanuts, strawberry, blueberry, cranberry, mulberry, lingberry, sparkleberry, bilberry and in flowers as well as leaves like butterfly orchid tree, eucalyptus, spruce, lily, gnetum and so many others. Functionally, RSV has the propensity to safeguard DNA as well as the induction of DNA repair. RSV is precipitously metabolized in the liver via phase-II detoxification enzymes leading to its principal urine excretion. RSV steered growth inhibition, induction of apoptosis and G0/G1phase cell cycle arrest in an experiment involving glioma cells. RVS can block the triggering of signal transducer and activator of transcription (STAT3) signaling of glioma cells. RSV subdues STAT3 signaling via the inhibition of SRC or Janus kinase (JAK2) induction, thereby inducing growth inhibitory and apoptotic properties. RSV explicitly blocks both COX-1 and COX-2 invitro. In the cancer inflammatory milieu, the blockade effects of RSV on NF-κB could also lead to the blockade of TNF-α resulting in inhibition of cancer advancement as well as metastasis.Individually, RSV has proven to very potent in glioma cells. It is able to down-regulate glioma angiogenesis as well as metastasis. In combination with other agents, RVS augment its potency in glioma. RVS is able to cross the blood brain barrier (BBB) via gap junctions making it very efficient central nervous system medication. RVS after oral administration peaks in the blood stream after one hour meaning it acts very fast. This review focuses on the neuropharmacological role of RVS in glioma.proven to have chemo-preventive effects on numerous human ailments like osteoporosis and gastric ulcers besides the cardioprotective effect mentioned above (27,32). STRUCTURE AND FUNCTIONS OF RESVERATROLRSV is a white powder with a feeble yellow cast. It is a stilbene with a natural polyphenol configuration analogous to diethylstilbestrol and estradiol (32,86). Its molecular formula is C 14 H 12 O 3 and it is commonly referred to as 3,5,4'thihydroxystilbene. Other unconventional terms use to describe this compound are: 3,4',5-stilbenetriol, (E)-5-(7-hydroxystyryl) resorcinol, (E)-5-(4-hydroxystyryl) benzene-1,3-diol. RSV occurs innately as both cis-and trans-isomers. The trans-isomer is often used in classic laboratory studies because its more physical available have has higher biological activity than the cis-isomer. On the other hand, the cis-isomer is unstably and not commercial accessible (32,144).Functionally, RSV has the propensity to safeguard DNA as well as the induction of DNA repair (17,89). Studies have proven that, at high dose, RSV does not only suppress cancer advancement but also inhibits the synthesis of RNA, DNA and

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Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 67%

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

Section: Effects Of Resveratrol On the Radiosensitivity Of Iododeoxyumentioning

confidence: 99%

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The Therapeutic Potential of Resveratrol in Gliomas

Richard¹

2019

ABCMed

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Effect of resveratrol on the radiosensitivity of 5‐FU in human breast cancer MCF‐7 cells

Aghamiri

Jafarpour

Zandsalimi

et al. 2019

J of Cellular Biochemistry

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The aim of this study was to assess the efficacy of resveratrol (Res) on radiosensitivity of 5‐fluorouracil (5‐FU) in the spheroid culture of MCF‐7 breast cancer cell line using colony formation examination. Spheroids on day 9 with 300 µm diameters were treated with 20 µM resveratrol and/or 1 µM 5‐FU for one volume doubling time (VDT) (42 hours) and then irradiated with 2 Gy gamma radiation (60Co) in various groups. Then the viability of the cells and clonogenic ability were acquired by blue dye exclusion and colony formation assay, respectively. The population doubling time in the monolayer culture and the VDT of spheroid culture was 22.48 ± 0.23 hours and 42 ± 0.63 hours respectively. None of the drugs and combination of them had any effect on the viability of cells. The combination treatment of 5‐FU+Res+ radiation significantly reduced the colony formation ability of spheroid cells in comparison with each treatment alone. Our results indicated that resveratrol can significantly decrease colony number of breast cancer spheroid cells treated with 5‐FU in combination with gamma‐rays. Thus, resveratrol as a hypoxia‐inducible factor‐1‐alpha inhibitor increased the radiosensitization of breast cancer spheroid cells.

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Comet assay: an essential tool in toxicological research

2016

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The comet assay is a versatile, reliable, cost-efficient, and fast technique for detecting DNA damage and repair in any tissue. It is useable in almost any cell type and applicable to both eukaryotic and prokaryotic organisms. Instead of highlighting one of the numerous specific aspects of the comet assay, the present review aims at giving an overview about the evolution of this widely applicable method from the first description by Ostling and Johanson to the OECD Guideline 489 for the in vivo mammalian comet assay. In addition, methodical aspects and the influence of critical steps of the assay as well as the evaluation of results and improvements of the method are reviewed. Methodical aspects regarding oxidative DNA damage and repair are also addressed. An overview about the most recent works and relevant cutting-edge reviews based on the comet assay with special regard to, e.g., clinical applications, nanoparticles or environmental risk assessment concludes this review. Taken together, the presented overview raises expectations to further decades of successful applications and enhancements of this excellent method.

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Effects of resveratrol and methoxyamine on the radiosensitivity of iododeoxyuridine in U87MG glioblastoma cell line

Cited by 14 publications

References 26 publications

The Therapeutic Potential of Resveratrol in Gliomas

The Therapeutic Potential of Resveratrol in Gliomas

Effect of resveratrol on the radiosensitivity of 5‐FU in human breast cancer MCF‐7 cells

Comet assay: an essential tool in toxicological research

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