Effects of Rhein Lysinate on H2O2-induced cellular senescence of human umbilical vascular endothelial cells

Lin, Yajun; Zhen, Yong‐Su; Wei, Jie; Liu, Bo; Yu, Zong-yuan; Hu, Gang

doi:10.1038/aps.2011.101

Cited by 31 publications

(24 citation statements)

References 21 publications

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“…or R. tanguticum Maxim), has received attention for its anti-aging effects in vitro (25). RHL is a novel compound, which was synthesized by our team.…”

Section: Discussionmentioning

confidence: 99%

“…RHL is a novel compound, which was synthesized by our team. Compared with rhein, RHL is easily dissolved in water (25,31).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study from our laboratory demonstrated that RHL possessed an anti-aging effect in vitro, which may be associated with its anti-oxidative properties (25). However, no studies to date have addressed the effect of RHL on the aging process in vivo.…”

Section: Introductionmentioning

confidence: 96%

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Effects of rhein lysinate on D-galactose-induced aging mice

Zhen

Lin

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the anti-aging effects of rhein lysinate (RHL), and to explore its mechanism of action in a D-galactose-induced aging mouse model. Aging was induced by D-galactose (100 mg/kg/day) that was subcutaneously injected to animals for 8 weeks. RHL was simultaneously administered once a day by intragastric gavage. The appetite, mental condition, body weight and organ index of the mice were monitored. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were determined, and the levels of malondialdehyde (MDA) in the liver, kidney and serum were measured by appropriate assay kits. Western blot analysis was used to detect proteins associated with age. The results indicated that RHL may improve the appetite, mental state and organ conditions of the model mice, improve the activities of SOD and GSH-Px, reduce MDA levels and modulate the expression of age-associated proteins (Sirtuin 1, p21 and p16) in D-galactose-induced mice. Therefore, RHL may be effective at suppressing the aging process through a combination of enhancing antioxidant activity, scavenging free radicals and modulating aging-associated gene expression.

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“…or R. tanguticum Maxim), has received attention for its anti-aging effects in vitro (25). RHL is a novel compound, which was synthesized by our team.…”

Section: Discussionmentioning

confidence: 99%

“…RHL is a novel compound, which was synthesized by our team. Compared with rhein, RHL is easily dissolved in water (25,31).…”

Section: Discussionmentioning

confidence: 99%

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Effects of rhein lysinate on D-galactose-induced aging mice

Zhen

Lin

et al. 2015

Experimental and Therapeutic Medicine

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“…Further, hydrogen peroxide (H2O2) is a principal mediator of ROS-dependent signaling, and exposure of endothelial cells to H2O2 induces the activation of ROS-dependent signaling cascades (Eligini et al, 2009). Incubation of HUVEC with 100 μmol/L H2O2 has been shown to induce oxidative stress, without inducing apoptosis (Lin et al, 2011), by inhibiting endogenous antioxidant activity and activating inflammatory signaling pathways (Hafizah et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Chew¹,

Mathison²,

Kimble³

et al. 2013

AJAFST

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Oxidative stress and inflammation are strongly linked to the development of cardiovascular disease (CVD). As a potent antioxidant, astaxanthin may downregulate inflammation-associated factors involved in endothelial dysfunction and CVD progression. We studied the possible protective effects of astaxanthin against endothelial dysfunction in human umbilical vein endothelial cells (HUVEC) induced with hydrogen peroxide (H2O2). Cells were pre-incubated with 0, 0.01, 0.1, or 1.0 μmol/L astaxanthin for 48 h, then oxidative stress induced with 100 μmol/L H2O2 overnight. Uptake kinetics of astaxanthin showed a time-dependent uptake of astaxanthin (1.0 μmol/L) by HUVEC over the 48 h incubation period. Oxidative stress induction with H2O2 in HUVEC decreased intracellular antioxidant activity and increased the production of inflammatory biomarkers and reactive oxygen species (ROS). In contrast, pre-incubation of HUVEC with astaxanthin prior to H2O2 stress increased (P < 0.05) superoxide dismutase activity and decreased ROS, prostaglandin E2, leukotriene B4, NO, IL-8, and IFN- production. Pre-treatment with astaxanthin also downregulated the transcriptional activation of NF-κB and activator protein-1, thereby inhibiting downstream production of inflammatory mediators and cytokines. Therefore, astaxanthin protects against factors initiated by H2O2 addition, likely by scavenging ROS required for transcriptional activation and inhibiting the production of inflammatory biomarkers involved in endothelial dysfunction and CVD.Abbreviations: AP-1: activator protein-1; CVD: cardiovascular disease; CS: culture supernatant; EGM-2: endothelial growth medium-2; GPx: glutathione peroxidase; H2O2: hydrogen peroxide; HUVEC: human umbilical vein endothelial cell; LTB4: leukotriene B4; PGE2: prostaglandin E2; ROS: reactive oxygen species; SOD: superoxide dismutase; THF: tetrahydrofuran.

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“…Rhein is isolated from the rhizome of rhubarb and is characterized by broad pharmacological effects, including antidiabetic activity, anti-inflammation and inhibition of interleukin-1-induced chondrocyte activation (11,12). However, its application is largely limited due to the poor water insolubility.…”

Section: Introductionmentioning

confidence: 99%

Improved heart failure by Rhein lysinate is associated with p38MAPK pathway

et al. 2018

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Abstract. The present study aimed to explore the role of Rhein lysinate (RHL) in neonatal rat ventricular myocytes (NRVMs) and congestive heart failure induced by co-arctation of the abdominal aorta. Male Sprague-Dawley rats were divided into 3 groups randomly: co-arctation of abdominal aorta group (A group, n=10), sham operation group (SH group, n=10) and RHL treatment rats (A+RHL group, n=10). To establish an in vitro oxidative stressed cardiomyocyte model, NRVMs were treated with 10 µM H 2 O 2 for 24 h. MTT assay indicated that H 2 O 2 treatment reduced primary cardiomyocyte viability in a time-and dose-dependent manner, whereas RHL abolished the detrimental effects of H 2 O 2 , indicating a protective role of RHL. Further study demonstrated that H 2 O 2 -induced reactive oxygen species (ROS) production was reversed by RHL. Then, TUNEL staining was carried out and the results revealed that H 2 O 2 markedly enhanced primary cardiomyocyte apoptosis. Conversely, RHL incubation decreased H 2 O 2 -induced cell apoptosis, indicating the protective role of RHL in primary cardiomyocytes. Furthermore, abnormal p38 activation was identified in the failed heart. Notably, treatment with RHL reduced p38 activation. In addition, RHL significantly enhanced the expression of anti-apoptotic protein, B cell lymphoma (Bcl)-2, however markedly reduced the protein level of Bcl-2 associated X, apoptosis regulator in primary cardiomyocytes, indicating its anti-apoptotic role in the cardiac setting. Overall, RHL protects heart failure primarily by reducing ROS production and cardiomyocyte apoptosis via suppressing p38 mitogen activated protein kinase activation.

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Effects of Rhein Lysinate on H2O2-induced cellular senescence of human umbilical vascular endothelial cells

Cited by 31 publications

References 21 publications

Effects of rhein lysinate on D-galactose-induced aging mice

Effects of rhein lysinate on D-galactose-induced aging mice

Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Improved heart failure by Rhein lysinate is associated with p38MAPK pathway

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