Effects of seasonal ambient heat stress on expression of microRNAs in the mammary gland of Holstein cows

Fan, Caiyun; Hu, Ruiting; Fan, Haixiao; Yang, Ying; Gong, Bin; Zhang, Shaoze; Ding, Jianping; Su, Yanjing; Zhao, Zhuo; Cheng, Jianbo

doi:10.1007/s00484-020-02025-5

Cited by 9 publications

(2 citation statements)

References 29 publications

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“…Conducting a study on 12 cows, Fan et al (2020) found 124 miRNAs both positively and negatively correlated with heat-stress stimulation, such as let-7c, let-7e, miR-181d, miR-452, and miR-31, which share the same target gene, IL-1, whose upregulation not only causes a systemic inflammatory response, but also impairs the health and physiological functions of dairy cows by altering blood sugar [259,260]. Thus, they concluded that these differentially expressed miRNAs may be involved in the development of udder inflammation under heat stress and, in contrast to the phenomenon observed by , promote the apoptosis of mammary gland cells, which ultimately affects milk composition and milk yield [261].…”

Section: Heat Stressmentioning

confidence: 98%

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

Dysin

Barkova

Pozovnikova

2021

ncRNA

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Milk is an integral and therefore complex structural element of mammalian nutrition. Therefore, it is simple to conclude that lactation, the process of producing milk, is as complex as the mammary gland, the organ responsible for this biochemical activity. Nutrition, genetics, epigenetics, disease pathogens, climatic conditions, and other environmental variables all impact breast productivity. In the last decade, the number of studies devoted to epigenetics has increased dramatically. Reports are increasingly describing the direct participation of microRNAs (miRNAs), small noncoding RNAs that regulate gene expression post-transcriptionally, in the regulation of mammary gland development and function. This paper presents a summary of the current state of knowledge about the roles of miRNAs in mammary gland development, health, and functions, particularly during lactation. The significance of miRNAs in signaling pathways, cellular proliferation, and the lipid metabolism in agricultural ruminants, which are crucial in light of their role in the nutrition of humans as consumers of dairy products, is discussed.

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Section: Heat Stressmentioning

confidence: 98%

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

Dysin

Barkova

Pozovnikova

2021

ncRNA

View full text Add to dashboard Cite

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“…Researchers have identified more than 1500 different miRNAs in bovine milk exosomes, with miR-148a, miR-30, miR-146, miR-200, miR-21, let-7, and others being common . The number and types of exosomal miRNAs in milk also change dynamically according to the breed of animals, lactation period, external environment, and seasonal changes . Although the biological functions of milk-derived miRNAs have not been fully confirmed; however, analysis by bioinformatic techniques predicts that most of them are involved in processes such as signal transduction, cell proliferation, and differentiation .…”

Section: Introductionmentioning

confidence: 99%

Bovine Colostrum miR-30a-5p Targets the NF-κB Signaling Pathway to Alleviate Inflammation in Intestinal Epithelial Cells

Zhang,

Zheng,

Han

et al. 2024

J. Agric. Food Chem.

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Inflammatory bowel disease (IBD) is a common disease of the digestive system, and an excessive immune response mediated by the nuclear factor κ-B (NF-κB) signaling pathway is an essential etiology. Recent studies have found that bovine milk exosomes can improve intestinal mucosal health by delivering microRNA (miRNA), but the mechanism of action is so far unknown.In the present study, we analyzed the differential expression profiles of miRNA in colostrum and mature milk exosomes using highthroughput sequencing, based on the demonstration that colostrum exosomes inhibit the lipopolysaccharide (LPS)-induced intestinal epithelial NF-κB inflammatory pathway better than mature milk exosomes. The bta-miR-30a-5p, which is specifically highly expressed in colostrum, was screened, and its predicted target gene TRAM was found to be closely related to the NF-κB signaling pathway by functional enrichment analysis. Further, we used gene overexpression and silencing techniques and found that the bta-miR-30a-5p transfection treatment was confirmed to inhibit LPS-induced NF-κB signaling pathway activation and downstream pro-inflammatory factor expression, while the expression of its potential target gene, TRAM, was also suppressed. It is hypothesized that the high expression of bta-miR-30a-5p in colostrum, which targets TRAM to inhibit the downstream NF-κB inflammatory pathway, may be one of the molecular mechanisms responsible for its superior effect on resisting inflammatory attack compared to mature milk.

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Circular RNA_015343 sponges microRNA‐25 to regulate viability, proliferation, and milk fat synthesis of ovine mammary epithelial cells via INSIG1

Wu,

Wang,

Hao

et al. 2024

Journal Cellular Physiology

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In our previous study, circ_015343 was found to inhibit the viability and proliferation of ovine mammary epithelial cells (OMECs) and the expression levels of milk fat synthesis marker genes, but the regulatory mechanism underlying the processes is still unclear. Accordingly in this study, the target relationships between circ_015343 with miR‐25 and between miR‐25 with insulin induced gene 1 (INSIG1) were verified, and the functions of miR‐25 and INSIG1 were investigated in OMECs. The dual‐luciferase reporter assay revealed that miR‐25 mimic remarkably decreased the luciferase activity of circ_015343 in HEK293T cells cotransfected with a wild‐type vector, while it did not change the activity of circ_015343 in HEK293T cells cotransfected with a mutant vector. These suggest that cic_015343 can adsorb and bind miR‐25. The miR‐25 increased the viability and proliferation of OMECs, and the content of triglycerides in OMECs. In addition, INSIG1 was found to be a target gene of miR‐25 using a dual‐luciferase reporter assay. Overexpression of INSIG1 decreased the viability, proliferation, and level of triglycerides of OMECs. In contrast, the inhibition of INSIG1 in expression had the opposite effect on activities and triglycerides of OMECs with overexpressed INSIG1. A rescue experiment revealed that circ_015343 alleviated the inhibitory effect of miR‐25 on the mRNA and protein abundance of INSIG1. These results indicate that circ_015343 sponges miR‐25 to inhibit the activities and content of triglycerides of OMECs by upregulating the expression of INSIG1 in OMECs. This study provided new insights for understanding the genetic molecular mechanism of lactation traits in sheep.

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Effects of seasonal ambient heat stress on expression of microRNAs in the mammary gland of Holstein cows

Cited by 9 publications

References 29 publications

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

Bovine Colostrum miR-30a-5p Targets the NF-κB Signaling Pathway to Alleviate Inflammation in Intestinal Epithelial Cells

Circular RNA_015343 sponges microRNA‐25 to regulate viability, proliferation, and milk fat synthesis of ovine mammary epithelial cells via INSIG1

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