Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet–Ozone Modified Polystyrene Surfaces

Teare, D. O. H.; Emmison, Neil; Ton-That, Cuong; Bradley, Robert H.

doi:10.1006/jcis.2000.7282

Cited by 36 publications

(38 citation statements)

References 27 publications

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“…Adherent cells then slowly (typically within hours) spread over the surface, depending on compatibility with the surface, expressing a strong 'biological component of adhesion' [38] that includes secretion of extracellular matrix (ECM) and results in the flattening of cells on the substratum [2] (spreadcell length is about 3-10 times height [9]). Needless to say, (protein) composition of the fluid phase can greatly affect the entire cell adhesion process [31,33,[55][56][57][58][59]. Thus, it is apparent that the short-term (<3 days) cell adhesion/proliferation process spans a broad range of time and length scales.…”

Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (α 2 , α 3 , α 4 , α 5 , α v , β 1 and β 3 ) integrins, vinculin, as well as secretion of osteopontin and type I collagen supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events -contact, attachment, spreading, and proliferation -are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum compatibility-superposition-principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cellsubstratum compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause-and-effect relationships between cell cell-substratum compatibility and substratum properties.Impact Statement-This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell

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Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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“…We chose mild UVO oxidation of PS because it may be applied with a high degree of control over the extent of oxidation, and because the identities and relative concentrations of newly formed surface species are well documented. 15,[23][24][25][26][27] Most reproducible results over the widest range of RH's were obtained for a PS surface that was exposed to UV light and ozone for t ox ) 75 s (Figure 3a). The thickness of the water layer increased approximately linearly (i.e., W ∝ RH) and by a factor of ∼2.2 as the humidity increased from ∼10% to ∼60% RH.…”

Section: The Initial Rate Of Charging Of a Stainless Steel Sphere Rolmentioning

confidence: 99%

“…The predominant species are alcohols or ethers, phenols (Ph-OR), 28 carboxylic acids or esters (ROs CdO), and aldehydes or ketones (R 2 CdO). 15,23,[25][26][27] The total concentration of the oxygen-containing species increases monotonically with time of exposure to UV light and ozone until a level of saturation is reached (∼36 atom %, determined by XPS, when t > 150 s). 15,25,26 Oxygen-containing species are present even on native surfaces of "unoxidized" PS (not UVO oxidized) because surface oxidation occurs naturally for samples aging under ambient laboratory conditions; 29 native PS petri dishes have typically ∼1 atom % oxygen on their surface.…”

Section: I) the Numbers Of Oxygen-containing Groups Present On The Sumentioning

confidence: 99%

Effects of Surface Modification and Moisture on the Rates of Charge Transfer between Metals and Organic Materials

et al. 2004

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This report is a study of the kinetics of contact charging resulting from the rolling of millimeter-sized metallic spheres on flat surfaces of polystyrene (PS). Charging was studied (i) for different values of the relative humidity (RH) of the air in contact with the system, (ii) in acidic and basic atmospheres, and (iii) for a series of PS surfaces oxidized to different degrees. The rates of charging increased with increasing RH; they were higher in basic atmospheres than in neutral or acidic ones and correlated with the numbers of hydrophilic groups on the surface of the polymer. These findings are consistent with a mechanism that implicates a thin film of water on the surface of PS as an important element of tribocharging in this system.

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“…Adherent cells then slowly (typically within hours) spread over the surface, depending on compatibility with the surface, expressing a strong 'biological component of adhesion ' [38] that includes secretion of ECM and results in the flattening of cells on the substratum [2] (spread-cell length is about 3-10 times height [9]). Needless to say, (protein) composition of the fluid phase can greatly affect the entire cell-adhesion process [31,33,[55][56][57][58][59]. Thus, it is apparent that the shortterm (o3 days) cell-adhesion/proliferation process spans a broad range of time and length scales.…”

Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

A New In Vitro Model of Breast Cancer Metastasis to Bone

Mastro¹,

Vogler²,

Gay³

2010

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)Penn State University University Park, PA 16802 PERFORMING ORGANIZATION REPORT NUMBER SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENTApproved for Public Release; Distribution Unlimited SUPPLEMENTARY NOTES ABSTRACTOsteoblasts (OB) grew into bone-like tissue in a 3D model system. Pre-OB matured to OB and eventually to osteocyte-like cells. These cells met the criteria of osteocytes based on shape and protein expression. In addition, the density was similar to that reported for calvaria bone. The 3-D system was used to examine the interaction of metastatic breast cancer cells, MDA-MB-231, with OB. The cancer cells brought about profound effects on the osteoid tissue. The OB changed from cuboidal to spindle shape.The cancer cells aligned into an "Indian filing" pattern, and penetrated the osteoid. Metastasis suppressed MDA-MB-231-BRMS cells attached loosely to the osteoblasts but did not colonize or penetrate the tissue. Using RT-PCR, we found that metastatic cancer cells downregulated OB differentiation proteins but increased inflammatory cytokines. We have tried to modify these effects by changing the oxidative status of the microenvironment with selenium and by adding drugs commonly used to treat metastatic breast cancer, zoledronc acid (ZOL). ZOL's main known target is the osteoclast but we found that it clearly affects osteoblasts and cancer cells. The OB morphology changes were inhibited. Selenium supplementation caused changes in the osteoblast cell morphology and permitted cancer cell growth but in a different pattern than seen in the deficient cultures. In summary the model mimics in vivo bone metastatic colonization. The results show that the osteblasts are a major target. SUBJECT TERMS INTRODUCTION:Breast cancer frequently metastasizes to the skeleton where it disrupts the balance between osteoblasts and osteoclasts and leads to osteolytic degradation . Th...

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Effects of Serum on the Kinetics of CHO Attachment to Ultraviolet–Ozone Modified Polystyrene Surfaces

Cited by 36 publications

References 27 publications

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Effects of Surface Modification and Moisture on the Rates of Charge Transfer between Metals and Organic Materials

A New In Vitro Model of Breast Cancer Metastasis to Bone

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