Effects of sex and estrous cycle on the brain and plasma arginine metabolic profile in rats

Zhang, Jiaxian; Yu, Jing; Zhang, Hu; Liu, Ping

doi:10.1007/s00726-021-03040-5

Cited by 9 publications

(4 citation statements)

References 77 publications

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“…This metabolic pathway affects aspartate, glutamate, and γ-aminobutyric acid (GABA) levels, which play key roles in early brain development (46). In addition, the involvement of the arginine biosynthesis and aspartate and glutamate metabolism has been identified in studies involving epilepsy, schizophrenia, AD animal models, and blood samples (47)(48)(49)(50). Our study provides a stronger validation of these conclusions.…”

Section: Effect Of Metabolite Ratios On Brain Cortex Thicknesssupporting

confidence: 60%

Association between human blood metabolites and cerebral cortex architecture: evidence from a Mendelian randomization study

Jiang,

Sun,

Liu

2024

Front. Neurol.

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BackgroundDysregulation of circulating metabolites may affect brain function and cognition, associated with alterations in the cerebral cortex architecture. However, the exact cause remains unclear. This study aimed to determine the causal effect of circulating metabolites on the cerebral cortex architecture.MethodsThis study utilized retrieved data from genome-wide association studies to investigate the relationship between blood metabolites and cortical architecture. A total of 1,091 metabolites and 309 metabolite ratios were used for exposure. The brain cortex surface area and cortex thickness were selected as the primary outcomes in this study. In this study, the inverse variance weighting method was used as the main analytical method, complemented by sensitivity analyses that were more robust to pleiotropy. Furthermore, metabolic pathway analysis was performed via MetaboAnalyst 6.0. Finally, reverse Mendelian randomization (MR) analysis was conducted to assess the potential for reverse causation.ResultsAfter correcting for the false discovery rate (FDR), we identified 37 metabolites and 9 metabolite ratios that showed significant causal associations with cortical structures. Among these, Oxalate was found to be most strongly associated with cortical surface area (β: 2387.532, 95% CI 756.570–4018.495, p = 0.037), while Tyrosine was most correlated with cortical thickness (β: −0.015, 95% CI −0.005 to −0.025, p = 0.025). Furthermore, pathway analysis based on metabolites identified six significant metabolic pathways associated with cortical structures and 13 significant metabolic pathways based on metabolite ratios.ConclusionThe identified metabolites and relevant metabolic pathways reveal potential therapeutic pathways for reducing the risk of neurodegenerative diseases. These findings will help guide health policies and clinical practice in treating neurodegenerative diseases.

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Section: Effect Of Metabolite Ratios On Brain Cortex Thicknesssupporting

confidence: 60%

Association between human blood metabolites and cerebral cortex architecture: evidence from a Mendelian randomization study

Jiang,

Sun,

Liu

2024

Front. Neurol.

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“…Rational Arg supplementation may alleviate E2 loss from Arg deficiency in women. 57 In all, compared with normal female mice, the effect of SCP on sex hormones seems to be amplified in POF female mice.…”

Section: Discussionmentioning

confidence: 92%

The evaluation of sea cucumber (Acaudina leucoprocta) peptide on sex hormone regulation in normal and premature ovarian failure female mice

et al. 2023

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“…Brain tissue collection was performed 2–3 days after the completion of the last behavioural test following a cerebral blood flow assessment [ 12 , 75 , 76 , 77 ]. Each animal was transcardially perfused with ice-cold saline, and the brain was rapidly removed and kept in cold saline (4 °C) for at least 45 s. The frontal cortex (FC), whole hippocampus (HP), parahippocampal region (PH containing the entorhinal, perirhinal and postrhinal cortices), striatum (ST) and cerebellum (CE) were dissected freshly on ice from each hemisphere, immediately snap-frozen on dry ice and stored at −80 °C for the HPLC and LC/MS assays, RT-qPCR and western blot.…”

Section: Methodsmentioning

confidence: 99%

“…The supernatant was collected and stored at −80 °C and the protein concentration was determined using the Bradford method [ 79 ]. The protein expression of arginase I and II, ASL, ASS and ODC in the parahippocampal region of PS19 mice in relation to their age-matched controls was then determined using western blots, as previously described [ 77 ]. All the samples were mixed with gel-loading buffer, containing 50 mM Tris-HCl, XT sample buffer (Bio-Rad Laboratories, 610791, Hercules, CA, USA) and reducing agent (Bio-Rad, 1610792), with the final protein concentration equalized to 2 mg/mL and boiled for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Altered Brain Arginine Metabolism and Polyamine System in a P301S Tauopathy Mouse Model: A Time-Course Study

Mein

Ahmad

et al. 2022

IJMS

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Altered arginine metabolism (including the polyamine system) has recently been implicated in the pathogenesis of tauopathies, characterised by hyperphosphorylated and aggregated microtubule-associated protein tau (MAPT) accumulation in the brain. The present study, for the first time, systematically determined the time-course of arginine metabolism changes in the MAPT P301S (PS19) mouse brain at 2, 4, 6, 8 and 12 months of age. The polyamines putrescine, spermidine and spermine are critically involved in microtubule assembly and stabilization. This study, therefore, further investigated how polyamine biosynthetic and catabolic enzymes changed in PS19 mice. There were general age-dependent increases of L-arginine, L-ornithine, putrescine and spermidine in the PS19 brain (particularly in the hippocampus and parahippocampal region). While this profile change clearly indicates a shift of arginine metabolism to favor polyamine production (a polyamine stress response), spermine levels were decreased or unchanged due to the upregulation of polyamine retro-conversion pathways. Our results further implicate altered arginine metabolism (particularly the polyamine system) in the pathogenesis of tauopathies. Given the role of the polyamines in microtubule assembly and stabilization, future research is required to understand the functional significance of the polyamine stress response and explore the preventive and/or therapeutic opportunities for tauopathies by targeting the polyamine system.

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Effects of sex and estrous cycle on the brain and plasma arginine metabolic profile in rats

Cited by 9 publications

References 77 publications

Association between human blood metabolites and cerebral cortex architecture: evidence from a Mendelian randomization study

Association between human blood metabolites and cerebral cortex architecture: evidence from a Mendelian randomization study

The evaluation of sea cucumber (Acaudina leucoprocta) peptide on sex hormone regulation in normal and premature ovarian failure female mice

Altered Brain Arginine Metabolism and Polyamine System in a P301S Tauopathy Mouse Model: A Time-Course Study

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