Effects of Simple and Disposable Chicken Cages for ExperimentalEimeriaInfections

Abstract

During experimental Eimeria infections in chickens, facilities are often contaminated by fecal oocysts known to be highly resistant to both chemical and enzymatic treatments. Thus, studies using experimental Eimeria infections have been limited due to the difficulty of complete elimination of residual oocysts from both cages and facilities. To overcome this limitation, simple, inexpensive, and disposable cages were constructed from cardboard boxes and tested during experimental Eimeria maxima infections. The cages were used in animal rooms with only a 1.7% evidence of coccidia contamination between adjacent cages. No significant differences in fecal oocyst output and body weight gain were noted between animals housed in disposable cages and animals housed in wire control cages. This cage design is a useful means for preventing oocyst contamination during experimental conditions, suggesting that this disposable cage design could be used for other avian infectious disease studies.

Identification and Comparative Expression Analysis of Interleukin 2/15 Receptor β Chain in Chickens Infected with E. tenella

“…Constant light was provided for the duration of the experiments. Ten-day-old chickens were orally infected with 1×10 4 sporulated E. tenella oocysts (Korean isolate 291–7) and transferred to disposable cages [53] . E. tenella were cleaned by flotation on 5.25% sodium hypochlorite and washed three times with PBS.…”