Effects of simple lipids on macrophages <i>in vitro</i>

Smith, A. Ian; Stuart, A. E.

doi:10.1002/path.1711150103

Cited by 14 publications

(8 citation statements)

References 13 publications

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“…At the dose employed, methyl palmitate does not alter the cellular architecture of the liver, lung, or spleen [9], which indicates that its tumor-potentiating activity may not be attributable to the destruction or lipid saturation of reticuloendothelial cells. Palmitic acid and its ethyl ester have been reported to depress leukocyte chemotactic and macrophage phagocytic ability in vitro [20,32]. Methyl palmitate administration may produce alterations in macrophage structure [33] and functional activity with a resultant decrease in macrophage infiltration of the tumor and a reduction in macrophage-mediated cytotoxicity, These possibilities have yet to be ascertained, The present study, in agreement with our previous observations [25,26], indicates that glucan administration increases serum lysozyme levels.…”

Section: Discussionsupporting

confidence: 94%

Comparative evaluation of macrophage stimulation and depression on tumor growth and macrophage content and function in mice

Luzio

Gilbert

Spanjers

1980

Cancer Immunol Immunother

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Section: Discussionsupporting

confidence: 94%

Comparative evaluation of macrophage stimulation and depression on tumor growth and macrophage content and function in mice

Luzio

Gilbert

Spanjers

1980

Cancer Immunol Immunother

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“…It can be inferred from these data that the RFc responses are modified by the lipid treatment in some way unrelated to the total amount of antigen in the spleen. Experiments with tricaprin [Stuart and Cooper, 1963;Smith and Stuart, 1975] [1968]. Specific tolerance in adult mice against sheep erythrocytes has been amply demonstrated, mostly using cyclophosphamide treatment [Playfair, 1969;Miller and Mitchell, 1970;Argyris, Haritou and Cooney, 1972;Aisenberg and Murray, 1973], which is known to damage lymphocytes [W. L. Ford, personal communication].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Cholesterol Oleate Treatment of Mice on the Rosette Forming Cell Response Against Sheep Erythrocytes

et al. 1976

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Mice injected with a single dose of 60 mg cholesterol oleate emulsion showed substantial blockade of the mononuclear phagocyte system, measured by the rate of vascular clearance of radio-labelled sheep erythrocytes. The Unanue, 1972;. Physiological investigation under in vivo conditions is made difficult by the scattered distribution of lymphoid tissues and the intermingling of macrophages with lymphocytes in these tissues, and the susceptibility of both cell types or their precursors to irradiation and cytotoxic agents. Nevertheless, as stressed by Gorczynski, Miller and Philips [1971], there is a need to test the inferences derived from in vitro studies by in vivo experiments.Modification of the phagocytic function of fixed mononuclear phagocytes is readily achieved by the intravenous injection of certain simple lipids [Di Luzio and Blickens, 1966;Stuart, 1970]. Emulsions of cholesterol oleate injected intravenously block the mononuclear phagocyte system for several days and have not been observed to have any effect on the ultrastructure of lymphocytes of the spleen and lymph nodes . No effect on cultured lymphocytes can, as yet, be attributed to treatment with this lipid .

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“…However, when we studied the macrophage migration in vitro, the same lipids proved to be only just as active stimulants of migration, as corresponding quantities of PMB supernatant. The stimulating action of triglycerides on macrophages in vitro was also described independently (68).…”

Section: Macrophage Breakdown and Control Of Phagocytosis Responsementioning

confidence: 96%

Response of a phagocyte cell system to products of macrophage breakdown as a probable mechanism of alveolar phagocytosis adaptation to deposition of particles of different cytotoxicity.

Privalova¹,

Katsnelson²,

Osipenko³

et al. 1980

Environ Health Perspect

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The adaptation of the alveolar phagocytosis response to the quantitative and qualitative features of dust deposited during inhalation consists not only in enhanced recruitment of alveolar macrophages (AM), but also in adding a more or less pronounced neutrophil leukocyte (NL) recruitment as an auxiliary participant of particle clearance. The NL contribution to clearance is especially typical for response to cytotoxic particles (quartz, in particular). An important feature of the adaptation considered is the limitation of the number of AM and NL recruited when an efficient clearance can be achieved by a lesser number ofceDls due to increased AM resistance to the damaging action of phagocytized particles. The main mechanism providing the adequacy of the alveolar phagocytosis response is its self-regulation through the products of macrophage breakdown (PMB). In a series of experiments with intraperitoneal and intratracheal injections of syngenetic PMB into rats and mice, it was shown that these products stimulate respiration and migration of phagocytic cells, their dose-dependent attraction to the site of PMB formation with the predominant NL contribution, increasing with the increase of amount of PMB, the AM and NL precursor cells recruitment from reserve pools, and the replenishment of these reserves in the process of hemopoiesis. At least some of the above effects are connected with the action of the lipid components of PMB. The action of specialized regulative systems of the organism can modify the response to PMB, judging by the results obtained by hydrocortisone injection. Autocontrol of alveolar phagocytosis requires great care in attempts at artificial stimulation ofthis process, as an excessive cell recruitment may promote the retention of particles in lungs.According to estimates based on comparison of calculated deposition of dust in miners' lungs with the actual amount found postmortem, about 98-99% of primarily deposited dust mass is cleared from the respiratory system (1). This impressive calculation allows us not only to explain why life is compatible

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Effects of simple lipids on macrophages in vitro

Cited by 14 publications

References 13 publications

Comparative evaluation of macrophage stimulation and depression on tumor growth and macrophage content and function in mice

Comparative evaluation of macrophage stimulation and depression on tumor growth and macrophage content and function in mice

The Effect of Cholesterol Oleate Treatment of Mice on the Rosette Forming Cell Response Against Sheep Erythrocytes

Response of a phagocyte cell system to products of macrophage breakdown as a probable mechanism of alveolar phagocytosis adaptation to deposition of particles of different cytotoxicity.

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