Effects of starvation and feeding on blood chemistry, fatty acid composition and expression of vitellogenin and fatty acid-binding protein genes in female swimming crab Portunus trituberculatus broodstock

Ding, Liyun; Fu, Huiyun; Hou, Yingmei; Jin, Min; Sun, Peng; Zhou, Qicun

doi:10.1007/s12562-017-1075-3

Cited by 14 publications

(5 citation statements)

References 80 publications

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“…Starvation effectively increased the whole‐body n‐3 and n‐6 PUFA levels (ARA, EPA and DHA), and ∑ n‐3/∑ n‐6 PUFA ratio of juvenile brown trout. The conservation of long chain‐PUFAs (LC‐PUFA) during food deprivation has also been reported for other teleosts (Barreto‐Curiel et al., 2017; DeSilva et al., 1997; Ding et al., 2017; Luo et al., 2009; Ölmez et al., 2015; Smith et al., 2003). LC‐PUFAs are the major components of cell membrane phospholipids, acting as initiators of prostaglandin and other eicosanoids.…”

Section: Discussionmentioning

confidence: 59%

Changes in fatty acids, blood biochemistry and mRNA expressions of genes involved in polyunsaturated fatty acid metabolism in brown trout (Salmo trutta) during starvation and refeeding

et al. 2020

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Section: Discussionmentioning

confidence: 59%

Changes in fatty acids, blood biochemistry and mRNA expressions of genes involved in polyunsaturated fatty acid metabolism in brown trout (Salmo trutta) during starvation and refeeding

et al. 2020

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“…The presence of PemVIH in CoG, a part of STNS, also suggests it may mediate the crosstalk between nutritional and reproductive signaling. For example, it may play a role in the previously reported stimulation of vitellogenesis by feeding and inhibition of vitellogenesis by starvation ( 54 , 55 ). In contrast, PemGIH is localized in the sinus gland and eyestalk ganglia of broodstock females ( 27 ).…”

Section: Discussionmentioning

confidence: 95%

Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

et al. 2021

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In this study, a novel Crustacean Hyperglycemic Hormone-type II gene (CHH-type II) was identified and biologically characterized in a shrimp, Penaeus monodon. Based on its structure and function, this gene was named P. monodon vitellogenesis-inhibiting hormone (PemVIH). The complete cDNA sequence of PemVIH consisted of 1,022 nt with an open reading frame (ORF) of 339 nt encoding a polypeptide of 112 amino acids. It was classified as a member of the CHH-type II family based on conserved cysteine residues, a characteristically positioned glycine residue, and the absence of CHH precursor-related peptide (CPRP) domain. The deduced mature PemVIH shared the highest sequence similarities with giant river prawn sinus gland peptide A. Unlike P. monodon gonad-inhibiting hormone (PemGIH), PemVIH was expressed only in the brain and ventral nerve cord, but not the eyestalks. Whole mount immunofluorescence using a newly generated PemVIH antiserum detected positive signals in neuronal cluster 9/11 and 17 of the brain, commissural ganglion (CoG), and neuronal clusters of ventral nerve cord. The presence of PemVIH-positive neurons in CoG, a part of stomatogastric nervous system, suggested a potential mechanism for crosstalk between nutritional and reproductive signaling. The role of PemVIH in vitellogenesis was evaluated using RNA interference technique. Temporal knockdown of PemVIH in female subadults resulted in a 3-fold increase in ovarian vitellogenin expression, suggesting an inhibitory role of PemVIH in vitellogenesis. This study provided novel insight into the control of vitellogenesis and additional strategies for improving ovarian maturation in P. monodon without the current harmful practice of eyestalk ablation.

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“…Current research shows that the maturation of ovary is accompanied by the increase of oocyte volume, and the main reason for the increase of oocyte volume is the accumulation of vitellin, so vitellogenesis is considered as the central link of ovarian maturation [1,17]. As a precursor of vitellin synthesis, VTG is mainly synthesized in adipocytes in hepatopancreas and ovary and then selectively absorbed by endocytosis mediated by VGR on the surface of oocytes [39]. The synthesis of VTG is regulated by endocrine hormones.…”

Section: Discussionmentioning

confidence: 99%

The Role of Cholesterol during the Ovarian Maturation and Lipid Metabolism of Female Chinese Mitten Crab (Eriocheir sinensis)

Guo,

Hua,

Wang

et al. 2024

Aquaculture Nutrition

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In previous study, we found that the cholesterol requirement of Eriocheir sinensis was 0.27%, to further investigate the effects of cholesterol on health status, ovarian maturation, and lipid metabolism of female Eriocheir sinensis broodstock. Two diets containing 0% and 0.25% (actually 0.05% and 0.27%) cholesterol were fed to the female crabs (average weight: 49.21 ± 0.11 g) for 4 months and sampled once a month. The results showed that the body weight (BW), survival rate (SR), meat yield (MY), condition factor (CF), hepatosomatic index (HSI), and gonadosomatic index (GSI) of Eriocheir sinensis were significantly affected by treatment time and compared with the cholesterol deficient group, supplementing cholesterol significantly increased BW, HSI, and GSI (P<0.05). In addition, long-term lack of cholesterol will lead to a significant decrease in the activity of ACP, AKP, and SOD and a significant increase in the content of MDA. The histological results showed that cholesterol significantly increased the volume of oocytes (P<0.05). Further studies found that 0.27% cholesterol significantly increased the transcription levels of vtg and vgr in hepatopancreas and ovaries, which may be the main reason for the increase of oocyte size (P<0.05). When fed with 0.27% cholesterol diet, the contents of nutrients in hepatopancreas and ovaries increased significantly, especially lipids and cholesterol (P<0.05). Through the analysis of mRNA expression level of genes related to lipid metabolism, it was found that cholesterol enhanced the transcription level of genes related to lipid synthesis and transport in hepatopancreas, thereby promoting the accumulation of lipid in the organism. Furthermore, compared with control group, the levels of juvenile hormone (JH), 17β-estradiol (E2), methyl farnesoate (MF), and ecdysone in the organism were significantly increased after feeding a diet with 0.27% cholesterol (P<0.05). In summary, supplementing an appropriate amount of cholesterol in the diet can improve the growth performance of Eriocheir sinensis broodstock, enhance the body’s antioxidant and immune system, and promote the accumulation of nutrients in the ovaries, thereby promoting ovarian maturation.

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Effects of starvation and feeding on blood chemistry, fatty acid composition and expression of vitellogenin and fatty acid-binding protein genes in female swimming crab Portunus trituberculatus broodstock

Cited by 14 publications

References 80 publications

Changes in fatty acids, blood biochemistry and mRNA expressions of genes involved in polyunsaturated fatty acid metabolism in brown trout (Salmo trutta) during starvation and refeeding

Changes in fatty acids, blood biochemistry and mRNA expressions of genes involved in polyunsaturated fatty acid metabolism in brown trout (Salmo trutta) during starvation and refeeding

Suppression of a Novel Vitellogenesis-Inhibiting Hormone Significantly Increases Ovarian Vitellogenesis in the Black Tiger Shrimp, Penaeus monodon

The Role of Cholesterol during the Ovarian Maturation and Lipid Metabolism of Female Chinese Mitten Crab (Eriocheir sinensis)

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