Effects of Storage in Different Commercial Semen Extenders on Sperm Motility, Viability and Membrane Integrity of Korean Native Boar Spermatozoa

Sa, Soo-Jin; Kim, In-Cheul; Choi, Suna; Hong, Ji‐Yeon; Kim, Du-Wan; Cho, Kyu-Ho; Kim, Young-Hwa; Chung, Ki-Hwa; Park, Jun-Cheol

doi:10.12750/jet.2013.28.4.349

Cited by 4 publications

(2 citation statements)

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“…It was also observed that the mean percentage of intact acrosome was significantly (P<0.05) higher in Modena than in GEPS at 72, 144 and 168 h of preservation. The present results were in close conformity with those reported by Kaeoket et al (2010) but higher than that reported by Sa et al (2013). The mean percentage of intact acrosome decreased significantly (P<0.05) with the increase in preservation time in all the extenders.…”

Section: Resultssupporting

confidence: 93%

Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C

SANGMA

Ahmed

CHOUDHURY

et al. 2020

Indian J of Anim Sci

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The present study was designed to compare the efficacy of 3 different extenders for preservation of boar semen at 15°C. Ejaculates (32) were evaluated and extended (1:4) in TRIXcell+, Modena and GEPS extenders and kept for 4 h of holding at 22°C. The samples were preserved in a BOD incubator at 15°C up to 168 h and evaluated for sperm motility, intact acrosome and HOST-reacted sperm at 0, 72, 96, 120, 144 and 168 h of preservation. The mean percentage of sperm motility, intact acrosome and HOST-reacted sperm was significantly higher in TRIXcell+ extender than Modena and GEPS at different hours of preservation. Out of the 3 extenders studied, TRIXcell+ was found to be the best followed by Modena and GEPS extenders respectively based on sperm motility, intact acrosome and HOST.

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Section: Resultssupporting

confidence: 93%

Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C

SANGMA

Ahmed

CHOUDHURY

et al. 2020

Indian J of Anim Sci

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“…2 Semen collected from boars is ultimately diluted in any one of a variety of commercially available boar semen extenders. Among the commercially available boar semen extenders are Beltsville TS, Androhep + , Mulberry III, Acromax, X-CELL, Seminark, Vitasem LD, etc, [3][4][5][6] and most of them are either short-or long-term preservation media. These extenders are used to create multiple insemination doses from a single ejaculate and contain buffers and nutrients, which provide spermatozoa a congenial environment that maintains their preservation capabilities and viability for 3 or more days postcollection.…”

Section: Introductionmentioning

confidence: 99%

Survivability of boar sperm stored under room temperature in extenders containing some natural products

Akandi¹,

Ugwu²,

Machebe³

2015

OAAP

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Background: Semen extenders are used to create multiple insemination doses from a single ejaculate and contain buffers and nutrients suitable for storage of spermatozoa. The purpose of this study was to evaluate the survivability of boar sperm stored under room temperature in different extenders containing some natural products. Methods: A volume of 10 mL of pooled semen collected from four mature crossbred (Landrace × Large White) boars was stored in 12 different extenders containing 1.0%, 1.5%, and 2.0% per liter each of honey, sugarcane juice, tomato juice, and pineapple juice. The semen samples were observed under a microscope every hour to determine sperm motility by scoring. Survivability of the sperm cells was taken as the average percentage number of motile spermatozoa in each extender recorded during 24 hour storage duration. The experimental layout was a 4×3 factorial in a randomized complete block design. Results: Average sperm survivability was higher for semen stored in honey and sugarcane juice extenders compared (P,0.05) to sperm stored in tomato and pineapple extenders. The proportion of spermatozoa that survived within the 24 hour storage time was highest in the honey group and lowest in the pineapple group. The average percentage of sperm that were motile was higher when 1.5% and 2.0% of test ingredients was included in extender compared (P,0.01) to 1.0% inclusion rate. Conclusion: The present study clearly demonstrates that spermatozoa can be stored in extenders containing honey, sugarcane juice, tomato juice, and pineapple juice. Moreover, the survivability of sperm in these extenders can be enhanced when the percentage constituent of these natural materials in the extenders is increased from 1.0% to 2.0% inclusion rates.

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In vitro effect of zearalenone on sperm parameters, oocyte maturation and embryonic development in buffalo

Yousef

Rezk

El-Naby

et al. 2023

Reproductive Biology

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Effects of Storage in Different Commercial Semen Extenders on Sperm Motility, Viability and Membrane Integrity of Korean Native Boar Spermatozoa

Cited by 4 publications

References 0 publications

Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C

Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C

Survivability of boar sperm stored under room temperature in extenders containing some natural products

In vitro effect of zearalenone on sperm parameters, oocyte maturation and embryonic development in buffalo

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