Effects of Sucrose and Trehalose on the Freezability of Markhoz Goat Spermatozoa

Abstract

The present study was conducted to observe the effect of increased osmolality of basic tris extender supplemented with trehalose and sucrose on post-thawing quality (motility, progressive motility, viability, the rate of acrosome abnormality, total abnormality and membrane integrity) of Markhoz goat spermatozoa. Fresh semen samples were evaluated for motility and sperm concentration. Only semen samples with motility more than 70% and sperm concentration higher than 3×10 9 sperm/ml were used for cryopreservation. In Exp. 1, trehalose (50, 75 or 100 mM) and sucrose (40, 60 or 80 mM) were added to a basic tris diluent. Based on the results of experiment 1, the goal of Exp. 2 was to investigate the combinational effects of the highest and lowest concentrations (T 100 +S 80 or T 50 +S 40 ) of trehalose and sucrose. As the control, semen was diluted and frozen in the tris diluent without trehalose or sucrose. The results in Exp. 1 showed that all evaluated spermatozoa characteristics improved significantly after freezing and thawing (p<0.05) and at the same time the increase of trehalose and sucrose concentrations in basic extenders was seen, with the best results obtained for extenders containing 70 and 100 mM trehalose and 80 mM sucrose. Comparing these results with those of control diluents, the effects of supplementation were significantly (p<0.05) better. In Exp. 2, the results showed no significant differences (p>0.05) between T 100 +S 80 and T 50 +S 40 extenders, but the results of T 50 +S 40 were slightly better than obtained with T 100 +S 80 diluents. Furthermore, the results of this experiment indicated that the sperm characteristics in the isotonic control extender were significantly (p<0.05) lower than examined extenders. In conclusion, the results of this study indicated that goat sperm can tolerate hypertonic trehalose and sucrose solutions better than isotonic control diluents in the freezing period. In particular, these positive effects have been shown for acrosome integrity, which is very important for the fertilization capacity of sperm. The data indicated that addition of trehalose plus sucrose to the freezing extender can be recommended for cryopreservation of goat spermatozoa, but more data is needed on pregnancy rate, acrosome reaction and IVF to ascertain the real effect.

The protease inhibitor antipain has a beneficial synergistic effect with trehalose for ram semen cryopreservation

“…Semen cryopreservation results in reduction of spermatozoa viability and fertility, due to multifactorial cryo‐damages (Holt, ). These cryo‐damages can be attenuated by modifying the composition of cryoprotectants in the extender (Hammerstedt, Graham, & Nolan, ; Khalili, Farshad, Zamiri, Rashid, & Fazeli, ). The results of the current study clearly revealed that supplementation of antipain (10 μM) with/without trehalose (30 and 60 mM) increased the quality of sperm after freezing.…”

Effects of DHA supplementation of the extender containing egg yolk and α- tocopherol on the freezability and post-thawing fertility of ram semen

“…The cryopreservation of spermatozoa is a complex process that causes several forms of cellular damages (Purdy, 2006). These damages has been attributed to cold shock, intercellular ice crystal, membrane alteration, and osmotic changes (Isachenko, 2003;Khalili et al, 2009), which may decrease motility and low fertility after thawing for artificial insemination (Matsuoka et al, 2006). Therefore, the cryoprotectants are included in the cryopreservation extenders to reduce the damaging effects of freezing processes (Purdy, 2006).…”

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ATPase isoform in sturgeon sperm cryopreservation

“…And Tsai, Chong, Meng, and Lin (2017) In support of this model, Molinia et al (1994) and Aisen et al (2002) have reported that the addition of a hypertonic extender maximized post-thaw motility of ram sperm. Others have suggested that the cryopreservation of goat sperm in hypertonic sucrose diluents was superior to that in isotonic extenders (Farshad & Akhondzadeh, 2008;Khalili, Farshad, Zamiri, Rashidi, & Fazeli, 2009 will lead to excessive dehydration of sperm and results in damage.…”