Effects of sucrose and trehalose on growth and development of protocorm-like bodies (PLBs) on two Dendrobium cultivars under different lights

Abstract

The objective of this study was to identify effective carbon sources for the in vitro propagation of PLBs in Dendrobium cultivars Dendrobium kingianum ‘Hallelujah’ and Dendrobium k. Jonathan’s Glory ‘Dark Joy’. In this study, we used two types of carbon sources to culture the cultivars under five different LED lights. For, both carbon sources the highest numbers of PLBs were obtained with the green LEDs compared with the other LED lights. For Dendrobium kingianum ‘Hallelujah’ the trehalose supplemented medium produced the highest number of PLBs (13.8/explant) and the maximum fresh weight (0.45g/explant) under green LEDs. On the other hand, for Dendrobium k. Jonathan’s Glory ‘Dark Joy’, the sucrose supplemented medium produced the highest number of PLBs under green LED (8.0/explant, fresh weight 0.22g/explants) and the trehalose supplemented medium produced the maximum number of PLBs under white LEDs (8.1/explants, fresh weight 0.23g/explants). The sucrose was the most relevant carbon source for the in vitro organogenesis of Dendrobium k. Jonathan’s Glory ‘Dark Joy’, while trehalose was best under white LEDs.