Effects of tannic acid on <i><scp>H</scp>aemonchus contortus</i> larvae viability and immune responses of sheep white blood cells <i>in vitro</i>

Zhong, Rongzhen; Liu, Hanwei; Zhou, Daowei

doi:10.1111/pim.12092

Cited by 21 publications

(18 citation statements)

References 41 publications

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“…Interestingly, Zhong et al . have recently noted a similar phenomenon in sheep leucocyte preparations exposed in vitro to Haemonchus contortus antigens, whereby enhanced IL‐10 production and reduced IL‐12 production were evident after co‐incubation of the cells with another type of tannin, tannic acid (a mixture of hydrolysable tannins that contain glucose as a central core that is surrounded by six or more galloyl groups), suggesting possibly a conserved interaction between helminth antigens and oligomeric polyphenols.…”

Section: Discussionmentioning

confidence: 73%

Co‐operative suppression of inflammatory responses in human dendritic cells by plant proanthocyanidins and products from the parasitic nematode Trichuris suis

et al. 2016

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Interactions between dendritic cells (DCs) and environmental, dietary and pathogen antigens play a key role in immune homeostasis and regulation of inflammation. Dietary polyphenols such as proanthocyanidins (PAC) may reduce inflammation, and we therefore hypothesized that PAC may suppress lipopolysaccharide (LPS) -induced responses in human DCs and subsequent T helper type 1 (Th1) -type responses in naive T cells. Moreover, we proposed that, because DCs are likely to be exposed to multiple stimuli, the activity of PAC may synergise with other bioactive molecules that have anti-inflammatory activity, e.g. soluble products from the helminth parasite Trichuris suis (TsSP). We show that PAC are endocytosed by monocyte-derived DCs and selectively induce CD86 expression. Subsequently, PAC suppress the LPS-induced secretion of interleukin-6 (IL-6) and IL-12p70, while enhancing secretion of IL-10. Incubation of DCs with PAC did not affect lymphocyte proliferation; however, subsequent interferon-γ production was markedly suppressed, while IL-4 production was unaffected. The activity of PAC was confined to oligomers (degree of polymerization ≥ 4). Co-pulsing DCs with TsSP and PAC synergistically reduced secretion of tumour necrosis factor-α, IL-6 and IL-12p70 while increasing IL-10 secretion. Moreover, both TsSP and PAC alone induced Th2-associated OX40L expression in DCs, and together synergized to up-regulate OX40L. These data suggest that PAC induce an anti-inflammatory phenotype in human DCs that selectively down-regulates Th1 response in naive T cells, and that they also act cooperatively with TsSP. Our results indicate a novel interaction between dietary compounds and parasite products to influence immune function, and may suggest that combinations of PAC and TsSP can have therapeutic potential for inflammatory disorders.

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Section: Discussionmentioning

confidence: 73%

Co‐operative suppression of inflammatory responses in human dendritic cells by plant proanthocyanidins and products from the parasitic nematode Trichuris suis

et al. 2016

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“…Tannin (in its condensed form) has an affinity for proline-rich proteins and the nematode cuticle is known to be a proline-rich structure that covers the body [ 16 ]. The proposed mechanisms of nematode control are that tannin disturbs the physiological processes of nematodes by binding to nematode proteins directly and subsequently blocking the parasites physiological processes and/or modifying host immune responses to eliminate infective larvae and adult worms [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus

Sambodo¹,

Prastowo²,

Kurniasih³

et al. 2018

Vet World

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Aim:Haemonchus contortus is a major problem in small ruminants in Indonesia. The frequent use of the anthelmintic drugs has given rise to drug-resistant populations which increase the need for new anthelminthic compounds, particularly from endemic plants. This study evaluated the in vitro effects of Biophytum petersianum crude aqueous extract (BAE) as an anthelmintic compound against H. contortus adult worm isolated from goats.Materials and Methods:Adult worm collected from naturally infected abomasums were obtained from slaughtered goats on the day of slaughter. BAE was prepared in six different concentrations (10, 20, 40, 60, 80, and 100 mg/ml) which were tested for their efficacies on ten actively moving worms. Ivermectin (1 mg/ml) was included as a reference drug, while saline water was included as a control. The dead worms from anthelmintic test then went through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscopy (SEM).Results:Highest mean mortality in treatments group both at 2 h and 4 h observations was BAE 10%. The SDS-PAGE analysis revealed the presence of five protein bands with molecular weights 9.3, 17.1, 50.0, 63.2, and 72.7 kDa based on BAE 10%. The SEM changes observed in the in vitro trials revealed the occurrence of interactions between the BAE and the cuticle.Conclusion:The SEM and SDS-PAGE analysis revealed ultrastructural structural changes and the decrease numbers of polypeptides on treated worms when compared to the control worms. It can thus be concluded that the BAE exhibits good anthelmintic activity against H. contortus adult worm.

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“…Tannin in G. apus can influence both directly and indirectly with adult worms. Direct reaction happens by attaching their cuticle and causing distress, while indirectly reaction happens by improving protein nutrition [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract

et al. 2018

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Aim:Haemonchus contortus is the most pathogenic nematode infesting the digestive tract of goats and sheep worldwide leading to a tremendous loss in a variety of routes. Economic losses due to haemonchosis in subtropic and tropic areas are usually caused by poor weight gain, minimized growth, loss of production, and mortality. The prevalence of haemonchosis in Indonesia is 89.4% in goat, and annual loss achieved 1 million US dollars. This study evaluated in vitro effects of Gigantochloa apus crude aqueous extract as an anthelmintic on H. contortus morphology and morphometry.Materials and Methods:Bligon goats which are naturally infected were collected from slaughtered goat from local slaughterhouses, namely Besi Sleman. Bligon goat’s abomasum part was carefully examined and transported to the Parasitology Laboratory, University of Gadjah Mada, Yogyakarta. H. contortus was obtained from 4 to 6-month-old female goat from slaughterhouses in Yogyakarta area. H. contortus was collected from abomasum and put into a Petri dish containing 0.62% water saline. The number of H. contortus used for each concentration is 25. H. contortus was soaked in each concentration for 4 h. The figure of the parasites or parts of parasites was captured using camera Lucida, and they were measured using both objective micrometer and objective ocular micrometer. All the capturing processes were done with the help of Olympus Digital Camera under Olympus CX21 microscopic. Parasite morphology was identified in morphological and morphometric characters.Result:Morphology of H. contortus revealed the cervical papillae bulge appears unclear shape and anterior end is more tapered. Vulvar flab control is not tapered, but vulvar flab which gets aware of G. apus crude aqueous extract looks more pointed. The gubernaculum appears irregular compared to gubernaculum control which tends to be more compact, and the posterior end form appears irregular more than posterior end control. Morphometry study of H. contortus indicates that it has a significant difference for body length, body width, cervical papillae, and spicule length in the male.Conclusion:G. apus crude aqueous extract activity revealed morphology change such as cervical papillae, vulvar flab, gubernaculum, posterior end, and reduced morphometry measurement of H. contortus adult worms, notably in body length, body width, cervical papillae width, gubernaculum, and spicule length in males and body length, body width, cervical papillae width, and vulva length in females.

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Effects of tannic acid on Haemonchus contortus larvae viability and immune responses of sheep white blood cells in vitro

Cited by 21 publications

References 41 publications

Co‐operative suppression of inflammatory responses in human dendritic cells by plant proanthocyanidins and products from the parasitic nematode Trichuris suis

Co‐operative suppression of inflammatory responses in human dendritic cells by plant proanthocyanidins and products from the parasitic nematode Trichuris suis

In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus

Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract

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