Effects of the cysteine protease inhibitor oryzacystatin (OC-I) on different aphids and reduced performance of Myzus persicae on OC-I expressing transgenic oilseed rape

Rahbé, Yvan; Deraison, Céline; Bonadé-Bottino, Michel; Girard, Cécile; Nardon, C.; Jouanin, Lise

doi:10.1016/s0168-9452(02)00402-8

Cited by 143 publications

(117 citation statements)

References 29 publications

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“…Similarly, soybean Kunitz trypsin inhibitor (SKTI) has also been shown to be effective against a range of pests (Johnston et al, 1993;. Oryzacystatin (OC-1), an inhibitor of cysteine proteinases derived from rice, has similarly been demonstrated to have insecticidal effects against aphids (Rahbe et al, 2003), coleopteran insects (Leple et al, 1995) and nematodes (Burrow et al, 1998;Cowgill et al, 2002) when expressed in crop plants, including potato and oilseed rape. Proteinase inhibitors expressed in transgenic plants, however, have the potential to adversely affect beneficial insects that feed on the target pest.…”

Section: Introductionmentioning

confidence: 99%

Digestive proteolytic activity in the gut and salivary glands of the predatory bug Podisus maculiventris (Heteroptera: Pentatomidae); effect of proteinase inhibitors

Bell¹,

Down²,

Edwards³

et al. 2005

Eur. J. Entomol.

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Abstract. Proteinase activity in the midgut of the pentatomid stinkbug Podisus maculiventris was investigated. The optimal pH for adult and nymph proteolysis was pH 6.0 and pH 6.5, respectively. Proteinase activity was characterised using a range of diagnostic inhibitors. Activity of both adult and nymphal gut extracts, detected by the hydrolysis of Z-Phe-Arg-pNA, was inhibited to <20% of control levels by several inhibitors (e.g. E-64 and chicken egg white cystatin) associated with the inhibition of cysteine proteinases. The less specific inhibitor leupeptin reduced proteolytic activity to around 1.0% of the control values. In-gel analysis of the enzymes revealed that proteolytic activity was due to at least four proteinases, of ca. 30, 36, 50 and 110 kDa, which were all susceptible to E-64 inhibition. Salivary gland extracts gave maximal activity at pH 8.0 when tested for general proteolytic activity using fluorescent BODIPY-FL casein substrate, and showed moderate levels of inhibition when incubated with inhibitors of serine-, cysteine-, aspartic-and metallo-proteinases. Leupeptin and PMSF gave the highest levels of inhibition of salivary proteolytic activity, at ca. 50%, whilst the plant-derived inhibitors SKTI, CpTI and OC-1 did not inhibit proteolysis.

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Section: Introductionmentioning

confidence: 99%

Digestive proteolytic activity in the gut and salivary glands of the predatory bug Podisus maculiventris (Heteroptera: Pentatomidae); effect of proteinase inhibitors

Bell¹,

Down²,

Edwards³

et al. 2005

Eur. J. Entomol.

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“…Although mechanistic details governing the uptake of dsRNA in aphids still remain elusive, host-mediated delivery of dsRNA is still an attractive paradigm for developing aphid resistance. Transgenics expressing aphid inhibitors have also shown deterrent effect against aphids in feeding trials (Carrillo et al, 2011;Tran et al, 1997;Rahbe et al, 2003). Present study was first time carried out in Gujrat, Pakistan to determine the presence of unigene sequences in local aphid species which will help further in the incorporation of RNAi in aphids via transgenic plants and to produce resistance crop line by using this method.…”

Section: Discussionmentioning

confidence: 91%

“…Transgenic crops are an option for insecticidal proteins production for e. g lectin and protease inhibitor (Wang et al, 2005;Hossain et al, 2006;Rahbe et al, 2003). Characterization and expression study of chitin-binding lectin, was associated with resistance against Hessian fly and grain aphid (Giovanini et al, 2007;Repellin et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Identifying potential RNAi targets in aphid species (Macrosiphum rosae, Rhopalosiphum maidis, Sitobion avenae, Toxoptera aurantii) in sub-tropical region with extreme summer

Afroz¹,

Hashmi²,

Rashid³

et al. 2017

Aust J Crop Sci

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RNA interference is a useful and efficient tool that had been used to incorporate tolerance against different stresses. Five unigene sequences were selected from exotic grain aphid that were reported to be an ideal RNAi targets. Aphids (Macrosiphum rosae, Rhopalosiphum maidis, Sitobion avenae, Toxoptera aurantii) were reared on Rosa indica, Zea mays, Triticum aestivum, Chrysanthemum hibiscus, Solanum melongena, Abelmoschus esculentus. PCR of unigene 28469 and 21789 was positive for all aphids and there is no ortholog found for them so unique and effective to be used in RNAi technology. Cytochrome c oxidase was found to be positive for M. rosae, S. avenae, T. aurantii and negative for R. maidis. Zinc finger protein was found to be positive for R. maidis, S. avenae and negative for M. rosae, and T. aurantii. Cuticular proteins was found to be positive for S. avenae and M. rosae and negative for R. maidis and T. aurantii. Genes identified in the aphids are defense and as important structural genes and their suppression with RNAi technology will be important target to have insect resistant crops. From these gene sequences cytochrome c oxidase is reported as bar coding gene and can be used in future for interspecific genetic variation in these aphids. Results revealed the high expression of these sequences in local aphid species and can be used as RNAi target for them. That can be used in future or applications as pest management, monitoring and plant quarantine.

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“…So, the old view that the aphids do not carry out hydrolysis of the ingested material inside the gut is no more valid (Pyati et al 2011). Major protease that the aphids use to digest proteins in the gut seems to be derived from both groups of cysteine and serine proteinases (Cristofoletti et al 2003;Rahbe et al 2003;Pyati et al 2011), although cysteine proteinases are likely to be more widespread (Terra & Ferreira 2005).…”

Section: Methodsmentioning

confidence: 99%

Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

Darvishzadeh¹,

Bandani²,

Mousavi³

2014

Plant Prot. Sci.

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Darvishzadeh A., Bandani A.R., Mousavi S.Q. (2014): Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae). Plant Protect. Sci., 50: 84-89.We identify and characterise α-amylases of the two aphid species, A. fabae and A. gossypi. To do this, α-amylases of the two insect species were extracted and their activities were determined using 1% soluble starch. Results showed that α-amylase, which hydrolyses starch, is present in both aphids. Also, it was shown that optimum pH and temperature for the α-amylases of both species is 7.0 and 40°C, respectively. Gel assays using zymogram analysis showed that in both aphid species more than one isoform (two isoforms) of α-amylases hydrolyszing carbohydrates are present.

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Effects of the cysteine protease inhibitor oryzacystatin (OC-I) on different aphids and reduced performance of Myzus persicae on OC-I expressing transgenic oilseed rape

Cited by 143 publications

References 29 publications

Digestive proteolytic activity in the gut and salivary glands of the predatory bug Podisus maculiventris (Heteroptera: Pentatomidae); effect of proteinase inhibitors

Digestive proteolytic activity in the gut and salivary glands of the predatory bug Podisus maculiventris (Heteroptera: Pentatomidae); effect of proteinase inhibitors

Identifying potential RNAi targets in aphid species (Macrosiphum rosae, Rhopalosiphum maidis, Sitobion avenae, Toxoptera aurantii) in sub-tropical region with extreme summer

Biochemical characterisation of α-amylase in two aphid species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

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