Effects of the Food Manufacturing Chain on the Viability and Functionality of Bifidobacterium animalis through Simulated Gastrointestinal Conditions

Abstract

The viability and functionality of probiotics may be influenced by industrial production processes resulting in a decrease in probiotic efficiency that benefit the health of humans. This study aimed to investigate the probiotic characteristics of Bifidobacterium strains isolated from fecal samples of healthy Thai infants. In the present work, three local strains (BF014, BF052, and BH053) belonging to Bifidobacterium animalis showed a great resistance against conditions simulating the gastrointestinal tract. Among these, B. animalis BF052 possessed considerable probiotic properties, including high acid and bile tolerance, strong adhesion capability to Caco-2 cells, and inhibitory activity against pathogens including Salmonella typhimurium and Vibrio cholerae. This strain also exhibited a high survival rate compared to commercial strains during storage in a wide variety of products, including pasteurized milk, soy milk, drinking yogurt, and orange juice. The impact of food processing processes as well as the freeze-drying process, storage of freeze-dried powders, and incorporation of freeze-dried cells in food matrix on probiotic properties was also determined. The stability of the probiotic properties of the BF052 strain was not affected by food processing chain, especially its resistance in the simulated gastrointestinal conditions and its adherence ability to Caco-2 cells. It indicates that it satisfies the criteria as a potential probiotic and may be used as an effective probiotic starter in food applications.

Comparative genomic analysis of the multispecies probiotic-marketed product VSL#3

“…animalis subsp. lactis strain BF052, a fecal isolate from healthy infants [ 75 ]. Blast analysis of the 32 spacer regions of L .…”

Bile acid patterns in commercially available oxgall powders used for the evaluation of the bile tolerance ability of potential probiotics

“…Due to the similarity between oxgall and human bile in bile acid composition, oxgall powder, a product derived from bovine bile, has been commonly used to assess the bile tolerance ability of potential probiotic strains at a concentration of 0.3% (wt/vol) instead of human bile [ 14 – 16 ]. In the international literature, the oxgall powders used for bacterial bile tolerance assay were mainly obtained from three manufacturers, namely Sigma-Aldrich (USA) [ 17 , 18 ], Oxoid (UK) [ 19 – 21 ] and BD Difco (USA) [ 22 , 23 ]. Little or no information has been reported on the total bile acid content and the content of the individual bile acids in commercially available oxgall powders.…”

Influence of Technological Treatments on the Functionality of Bifidobacterium lactis INL1, a Breast Milk‐Derived Probiotic

“…For example, it has been postulated that osmotic shock or formation of intracellular ice may affect some properties of probiotics. As for freeze drying, whereas no significant differences in the adhesion capability to Caco‐2 cells or in the resistance to simulated gastrointestinal transit of B. animalis BF052 were detected among lyophilized and nonlyophilized cells (Charnchai and others ), du Toit and others () found that freeze drying of probiotics, included B. lactis Bb12, had an effect on adhesion potential, showing an overall significant decrease in the ability of the lyophilized probiotic to exclude, displace, or outcompete certain pathogens. IL‐10 induction was only evident in animals treated with the lyophilized culture in the large intestine.…”