Effects of the oxygen transfer rate on ferrous iron oxidation by Thiobacillus ferrooxidans

Savić, Dragiša S.; Veljković, Vlada B.; Lazić, Miodrag L.; Vrvić, Miroslav M.; Vučetić, J.

doi:10.1016/s0141-0229(98)00071-4

Cited by 18 publications

(9 citation statements)

References 4 publications

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“…On the other hand, CO 2 may become the limiting factor of the growth with normal air (Boon et al, 1998). The mesophilic oxidation rate of ferrous iron with normal air was limited by the oxygen transfer rate, which was also been reported by Savić et al (1998). A gas mixture containing 99.5% O 2 / 0.5% CO 2 (vol/vol) was required for high-rate aeration in our laboratory-scale experiments.…”

Section: Discussionsupporting

confidence: 72%

High‐rate ferric sulfate generation by a Leptospirillum ferriphilum‐dominated biofilm and the role of jarosite in biomass retainment in a fluidized‐bed reactor

Kinnunen

Puhakka

2004

Biotech & Bioengineering

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The aims of this work were to develop a high-rate fluidized-bed bioprocess for ferric sulfate production, to characterize biomass retention, and to determine the phylogeny of the enrichment culture. After 7 months of continuous enrichment and air aeration at 37 degrees C, the iron oxidation rate of 8.2 g Fe(2+) L(-1)h(-1) (4.5.10(-12) g Fe(2+) cell(-1) h(-1)) was obtained at a hydraulic retention time (HRT) of 0.6 h. However, oxygen supply became the rate-limiting factor. With gas mixture (99.5% O(2)/0.5% CO(2) (vol/vol)) aeration and HRT of 0.2 h, the iron oxidation rate was 26.4 g Fe(2+) L(-1)h(-1) (1.0.10(-11) g Fe(2+) cell(-1) h(-1)). Leptospirillum sp. was predominant in the mesophilic fluidized-bed reactor (FBR) enrichment culture as determined by fluorescent in situ hybridization, while Acidithiobacillus ferrooxidans was not detected. Denaturing gradient gel electrophoresis (DGGE) of the amplified partial 16S rDNA showed only three bands, indicating a simple microbial community. DGGE fragment excision and sequencing showed that the populations were related to L. ferriphilum (100% similarity in sequence) and possibly to the genus Ferroplasma (96% similarity to F. acidiphilum). Jarosite precipitates accumulated on the top of the activated carbon biomass carrier material, increasing the rate of iron oxidation. The activated carbon carrier material, jarosite precipitates, and reactor liquid contained 59% (or 3.71.10(9) cells g(-1)), 31% (or 3.12.10(10) cells g(-1)) and 10% (or 1.24.10(8) cells mL(-1)) of the total FBR microbes, respectively, demonstrating that the jarosite precipitates played an important role in the FBR biomass retention.

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Section: Discussionsupporting

confidence: 72%

High‐rate ferric sulfate generation by a Leptospirillum ferriphilum‐dominated biofilm and the role of jarosite in biomass retainment in a fluidized‐bed reactor

Kinnunen

Puhakka

2004

Biotech & Bioengineering

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“…Four geochemical conditions for each of the five microbial treatments were established: (1) microaerophilic, (2) iron‐enriched microaerophilic, (3) oxygenated and (4) iron‐enriched oxygenated. Flasks were left static on the laboratory bench for microaerophilic treatments (1 and 2) using an experimental liquid to volume of flask ratio of 0.88, which has been shown to result in oxygen‐limited conditions after approximately 50 h (Savic et al ., 1988). Oxygenated treatments (3 and 4) were shaken at 175 r.p.m.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…For and Fe 2+ (to monitor Fe 3+ use as e‐ acceptor in experimental treatments) analyses, 1.5 mL aqueous aliquots were collected into autoclaved Eppendorf tubes from each flask replicate under sterile conditions at time = 0, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 2 weeks, 3 weeks and 4 weeks and stored at –20 °C. While the oxygenated samples were well mixed, heterogeneous oxygen regimes may have occurred in the microaerophilic treatments (oxygen limited after ~50 h (Savic et al ., 1998)). In order to minimize any potential variability in samples due to flask O 2 concentrations, samples were always taken from the bottom of the flasks where the most oxygen‐limiting conditions would presumably occur.…”

Section: Experimental Methodsmentioning

confidence: 99%

Microbial thiosulphate reaction arrays: the interactive roles of Fe(III), O₂ and microbial strain on disproportionation and oxidation pathways

Warren¹,

Norlund²,

Bernier³

2008

Geobiology

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In this work, we experimentally evaluate pH and SO4(2-) dynamics associated with abiotic and microbial S2O3(2-) oxidation under varying [O2], [Fe(III)] and microbial strain/consortia (two pure strains, Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, their consortia, and two enrichments from an acidic environmental system, Moose Lake 2002 and Moose Lake 2003). Results of the batch experiments demonstrate highly active microbial processing of S2O3(2-) while abiotic controls under identical experimental conditions remain static with no pH decrease. When abiotic controls were manually titrated with acid to achieve similar pH decreases to those occurring in the microbial treatments, different S pathways were involved. In particular, disproportionation is a substantial component of initial microbial S2O3(2-) processing, and is accelerated by the presence of Fe(III), indicating that recycling of S through intermediate oxidation states is likely to be widespread in acidic mine environments where high [Fe(III)] is common. Furthermore, the microbially mediated S reaction pathways were dependent on both environmental conditions and microbial strain/consortia, indicating that microbial community structure also plays a key role. Collectively, these results highlight the importance of microbial activity, their poor representation by abiotic S models, the likelihood that Fe(III), rather than O2, is a key control on microbial S processing in acid environments and the need to identify the microbial community/strain involved.

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“…The specific surface characteristics of materials could affect the cell immobilization and so the increasing immobilization performance leads to increase Fe 2 + biooxidation. However Fe 2 + biooxidation by A. ferrooxidans cells has been performed in different bioreactors using these materials including the air-lift, fluidized-bed [10,11], packed-bed bioreactors [12][13][14] and bubble columns [15]. The packed-bed reactor is an attractive choice because it is simple and cheap to install and operate [16].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Acidithiobacillus ferrooxidans on sulfonated microporous poly(styrene–divinylbenzene) copolymer with granulated activated carbon and its use in bio-oxidation of ferrous iron

Köseoğlu-İmer

Keskinler

2013

Materials Science and Engineering: C

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Effects of the oxygen transfer rate on ferrous iron oxidation by Thiobacillus ferrooxidans

Cited by 18 publications

References 4 publications

High‐rate ferric sulfate generation by a Leptospirillum ferriphilum‐dominated biofilm and the role of jarosite in biomass retainment in a fluidized‐bed reactor

High‐rate ferric sulfate generation by a Leptospirillum ferriphilum‐dominated biofilm and the role of jarosite in biomass retainment in a fluidized‐bed reactor

Microbial thiosulphate reaction arrays: the interactive roles of Fe(III), O₂ and microbial strain on disproportionation and oxidation pathways

Immobilization of Acidithiobacillus ferrooxidans on sulfonated microporous poly(styrene–divinylbenzene) copolymer with granulated activated carbon and its use in bio-oxidation of ferrous iron

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Effects of the oxygen transfer rate on ferrous iron oxidation by Thiobacillus ferrooxidans

Cited by 18 publications

References 4 publications

High‐rate ferric sulfate generation by a Leptospirillum ferriphilum‐dominated biofilm and the role of jarosite in biomass retainment in a fluidized‐bed reactor

High‐rate ferric sulfate generation by a Leptospirillum ferriphilum‐dominated biofilm and the role of jarosite in biomass retainment in a fluidized‐bed reactor

Microbial thiosulphate reaction arrays: the interactive roles of Fe(III), O2 and microbial strain on disproportionation and oxidation pathways

Immobilization of Acidithiobacillus ferrooxidans on sulfonated microporous poly(styrene–divinylbenzene) copolymer with granulated activated carbon and its use in bio-oxidation of ferrous iron

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Microbial thiosulphate reaction arrays: the interactive roles of Fe(III), O₂ and microbial strain on disproportionation and oxidation pathways