Effects of topical melatonin and vitamin E in a rat ischemic wound model

Abstract

Objective: Reactive oxygen species are known to increase on a chronic wound background. We therefore investigated the possible efficacy of the topical administration of melatonin and vitamin E, known to have radical scavenging properties, in the ischemic wound model.Methods: Forty Sprague-Dawley type adult male rats were divided into 4 groups as normal wound, ischemic group, and with vitamin E or melatonin applied to the ischemic wound. Bipedicular flap surgery to the shaved back of the rats was used to induce ischemia. A full-thickness skin lesion was produced by punch biopsy in each animal 3 days after this procedure. The punch biopsy procedure was performed without the flap surgery in the normal wound group. The vitamin E and melatonin administration continue twice a day for a total of 7 days after the wound was formed.Results: Hydroxyproline (OH-proline) and malonyldialdehyde (MDA) levels and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme activities were measured on the wound tissues removed by excisional biopsy at the end of the procedure. The MDA levels were significantly decreased in the groups receiving vitamin E and melatonin compared to the ischemic wound group. Vitamin E application also significantly increased OH-proline levels in the ischemic wounds. The antioxidant enzyme activities were not seen to be affected by the treatment procedures used.Conclusion: We concluded that the collagen synthesis decreased together with increased oxidative stress in the induced ischemic wound model; topical vitamin E application could reverse both states and melatonin could not support collagen synthesis although it had an antioxidant effect.

Protective Effects of Melatonin and S-Methylisothiourea on Mechlorethamine Induced Nephrotoxicity

“…Additionally, it is a potent ROS-scavenger [17][18][19]. MEL and its metabolites have also powerful anti-inflammatory properties and have proven to be highly effective in a variety of disorders linked to oxidative stress and inflammation in experimental animals [17].…”

The effects of topical melatonin on oxidative stress, apoptosis signals,and p53 protein expression during cutaneous wound healing

“…However, it showed antioxidant efficacy through decreasing MDA levels (Ozler et al, 2011). Although melatonin was administered at an amount of 3 mg per wound in the present study, in the study conducted by Ozler et al (2011), melatonin was administered at an amount of 0.1 mg per wound. Similar to our results, topical administration of melatonin (5 mg melatonin per rat and twice a day) decreased both MDA and MPO level in injured skin in rats with pressure ulcers (Sener et al, 2006).…”

“…Ozler et al (2011) showed that topically applied melatonin (5 mg/kg) in ischemic wounds had neither a positive nor a negative effect on hydroxyproline levels. However, it showed antioxidant efficacy through decreasing MDA levels (Ozler et al, 2011). Although melatonin was administered at an amount of 3 mg per wound in the present study, in the study conducted by Ozler et al (2011), melatonin was administered at an amount of 0.1 mg per wound.…”

“…Increased MPO activity in wound tissue suggests neutrophil accumulation and contributes markedly to tissue damage (Sener et al, 2006). Ozler et al (2011) showed that topically applied melatonin (5 mg/kg) in ischemic wounds had neither a positive nor a negative effect on hydroxyproline levels. However, it showed antioxidant efficacy through decreasing MDA levels (Ozler et al, 2011).…”

“…Simple ointment (Basiscreme™, Genesis, Turkey) was applied to the control group and 3% (w/w) melatonin ointment was applied to the treatment group (~3 mg/ wound), administered to each wound twice a day (Sener et al, 2006, Ozler et al, 2011 after wound creation during the experimental periods. Rats were sacrificed at the end of the experiment under anesthesia with ketamine and wound tissue samples were collected for the evaluation of oxidant/antioxidant status, collagen content, and apoptotic changing.…”