Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen

Abstract

Objectives: This study was designed to examine the effects of various concentrations of tris (hydroxymethyl) aminomethane (tris) and egg yolk on the quality of cryopreserved buck sperm. Materials and Methods: The collected semen samples were pooled, washed, and diluted into five different freezing extender groups, viz., extender I (tris 0% + egg yolk 0%), extender II (tris 1.41% + egg yolk 4%), extender III (tris 2.41% + egg yolk 8%), extender IV (tris 3.41% + egg yolk 16%), and extender V (tris 4.41% + egg yolk 24%). The sperm parameter of the five groups of extenders was evaluated after equilibration and cryopreservation. Results: The results showed that extenders II–V provided significantly higher semen progressive motility and total motility percentages than extender I after equilibration (p < 0.05). The higher percentages of semen progressive motility, total motility, viability, and plasma membrane integ¬rity (by both HOST under light microscopy and stain after HOST under light microscopy) were found in the sperm cryopreserved with extender IV than extender I, extender II, and extender III groups after thawing (p < 0.05). In addition, semen progressive motility, total motility, and viability were not further increased, or plasma membrane integrity (by both HOST tests) was decreased by the addition of tris and egg yolk (extender V) after cryopreservation (p < 0.05). Conclusion: In conclusion, our result indicates that the following washing, the supplementation of tris (3.41% + egg yolk 16%) on the freezing extender are suitable for improving the semen quality of buck after freezing and thawing.

Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat

“…At first, a [tris-citric-acid-fructose(TCF)] solution was made using our prior study’s [ 2 ] methodology, with a few modifications: tris-3.41 gm (Himedia Lab. Pvt., Ltd. Mumbai 400086, India.,), citric acid-1.61 gm (Sigma-Aldrich Corp., St. Louis, MO 63103, USA), fructose-0.81 gm (Himedia Lab.…”

“…The semen collection and selection parameters were carried out, according to Kamal et al [ 2 ], with some modifications. To summarize, a doe stimulated the buck, and semen was collected twice a week (at around 5-day intervals) using an artificial vagina.…”

“…The SPM and STM were examined by our prior investigation [ 2 ], with certain modifications. In brief, the SPM and STM were assessed after 10 μl of semen were diluted with the prewarmed (at 37°C) washing solution.…”

Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary (Pecari tajacu) Semen after Cooling Storage in a Transport Container at 5 °C

“…This efficiency may be related to the basic components of this medium, as TRIS has been used as an efficient pH regulator [ 36 ]. Furthermore, the phospholipids present in egg yolk have beneficial binding and protective properties for the plasma membrane [ 37 ], acting as non-permeable cryoprotectants [ 38 ] and protecting the functional integrity of sperm against thermal shock and cryoinjury [ 39 , 40 ]. However, during its use for the conservation of collared peccary semen, oscillations in the maintenance of progressive sperm motility were observed, probably because of the addition of egg yolk, which contains large particles without complete solubility, which can cause the formation of excessive debris that can interfere with the functional aspects of the semen [ 41 ].…”

“…The aforementioned efficiency of the medium in preserving these sperm parameters is mainly due to its components. TRIS has egg yolk as its main functional component, which plays a direct role in defending the plasma membrane against temperature-related damage [ 42 ], guaranteeing the maintenance of plasma membrane integrity and protecting against cryolesions [ 38 , 43 ]. For PRIMXcell Ultra, IMV Technologies have developed additives (not detailed) that preserve the main organelles of the sperm, such as mitochondria, acrosome, and plasma membrane, guaranteeing their viability and protecting the integrity of the sperm until oocyte fertilization [ 19 ].…”