Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa

Abstract

Tris (hydroxymethyl) aminomethane (Tris) has been used as a pH regulator for buffering the pH of dilution extenders for boar semen, such as the Modena extender. The purpose of the present study was to assess the effects of Tris supplementation at different concentrations (0, 8, 24 and 72 μM) into the freezing extender on the quality and fertilising capacity of frozen-thawed boar spermatozoa. The results showed that the supplementation of 24 μM of Tris gave significantly higher percentages of sperm viability and plasma membrane integrity than those of the control group at any time point of assessment (0 h and 3 h post-thawing) (P < 0.05). However, there were no significant differences in the acrosome integrity parameter among the groups. Higher percentages of sperm motility were observed in the spermatozoa cryopreserved with 24 μM of Tris compared to the control groups when the samples were analysed 0 h after thawing (P < 0.05). However, an increase of the Tris concentration to 72 μM did not enhance the sperm motility parameters. The total numbers of fertilised oocytes and blastocysts obtained with spermatozoa frozen with 24 μM Tris were significantly higher than those of the control group without Tris (P < 0.05). In conclusion, the supplementation of 24 μM Tris into the freezing extender contributes to a better boar sperm quality and fertilising capacity after the process of freezing and thawing.

Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary (Pecari tajacu) Semen after Cooling Storage in a Transport Container at 5 °C

“…These kinetic parameters are directly related to the ability of the sperm to fertilize [ 35 ]. This efficiency may be related to the basic components of this medium, as TRIS has been used as an efficient pH regulator [ 36 ]. Furthermore, the phospholipids present in egg yolk have beneficial binding and protective properties for the plasma membrane [ 37 ], acting as non-permeable cryoprotectants [ 38 ] and protecting the functional integrity of sperm against thermal shock and cryoinjury [ 39 , 40 ].…”

Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen

“…Fructose is the major carbohydrate source in bovine, including buffalo semen (Juyena & Stelletta, 2012). Tris (hydroxymethyl) aminomethane is a universal buffer suitable for preserving semen from various livestock (Namula et al, 2019). The results showed that the three commercial diluents with different lecithin sources had the same ability to protect the sperm plasma membrane during freezing and thawing.…”

Effects of tris (hydroxymethyl) aminomethane and egg yolk on the cryopreservation of buck semen

“…During semen cryopreservation, any changes in pH could affect the metabolism rates, motility, viability, and plasma membrane integrity [ 34 , 35 ]. Sodium citrate, HEPES, bicarbonate, and tris have been used as pH controllers to maintain the pH in the extender [ 9 , 19 , 25 ]. The pH of tris is representative of most living cells.…”

“…It has the advantage of being much less expensive than any other buffering agent and being highly soluble in water. In mammals, tris has been applied in the semen extender as a practical pH regulator that prevents the alteration of the hydroxide ion and hydronium ion concentration, protecting the cell against pH changes [ 19 ].…”

“…In addition, the pH of semen was decreased by the glycolytic pathway, which is the leading mechanism for energy production in semen, resulting in a decrease in metabolism and motility [ 8 ]. So, it is important to make the semen extender close to or slightly alkaline (like using tris) the environment of the semen of the body to overcome acidosis during freezing [ 19 , 54 ].…”