Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla

Peters, Ove A.; Galicia, Johnah C.; Arias, Ana; Tolar, Miroslav; Ng, Ernest Hung Yu; Shin, Su-Jung

doi:10.1111/iej.12571

Cited by 21 publications

(29 citation statements)

References 29 publications

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“…On the contrary, TotalFill downregulated ALP at 3 d. The osteogenic gene, OPG , was upregulated after exposure to Biodentine and TotalFill at 1 and 3 d, whereas MTA did not have any effect on OPG but upregulated expression of COL1A1 at 7 d. The angiogenic marker, VEGFA , was upregulated by MTA and TotalFill at 1 d of exposure, with this effect lasting longer for TotalFill. Some results obtained in this study were in agreement with those presented in previous reports and some were novel as not many reports compare these three TSCs. The osteogenic markers investigated are all implicated in osteogenic differentiation, dentine and mineral formation, regulation of osteoblastic differentiation, and osteogenesis .…”

Section: Discussionsupporting

confidence: 93%

“…The osteogenic markers investigated are all implicated in osteogenic differentiation, dentine and mineral formation, regulation of osteoblastic differentiation, and osteogenesis . The angiogenic markers chosen are vital for enhancement of endothelial cell proliferation, migration, and differentiation . Expression of these molecules is crucial in the healing process of the periodontium after endodontic procedures requiring use of TSC, such as perforation repairs and apicoectomies.…”

Section: Discussionmentioning

confidence: 99%

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Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Ali

Mustafa

Bårdsen

et al. 2019

European J Oral Sciences

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Tricalcium silicate cements (TSCs) are used in endodontic procedures to promote wound healing and hard tissue formation. The aim of this study was to evaluate and compare the effect of commonly used TSCs [mineral trioxide aggregate (MTA), Biodentine, and TotalFill] on cellular metabolism and osteogenic/angiogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) in vitro. We tested the null hypothesis of no difference between MTA, Biodentine, and TotalFill in stem cell responses. Cells were subjected to eluates of the tested materials for up to 14 d. Cell viability was evaluated using the 3‐(4,5‐dimethyl‐thiazoyl)‐2,5‐diphenyl‐tetrazolium bromide (MTT) assay. Real‐time PCR was used to determine the levels of expression of the osteogenic factors alkaline phosphatase (ALP), osteoprotegerin (OPG), osteocalcin (OC), and collagen 1A (COL1A1), and the angiogenic factors vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1). ELISAs were used to measure the levels of VEGFA and ALP in culture supernatants. Mineralization in vitro of hBMSCs was assessed using Alizarin Red staining. The hBMSCs tolerated exposure to TSCs well, with Biodentine showing the most favorable effect on cell viability. Expression of ALP, COL1A1, OPG, and VEGFA were differentially affected by the materials, with Biodentine and TotalFill inducing earlier changes at gene level. Increased mineralization was observed with time, after exposure to all TSCs tested, with MTA showing the greatest effect. The results revealed different responses of hBMSCs to TSCs in vitro.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Ali

Mustafa

Bårdsen

et al. 2019

European J Oral Sciences

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“…Acknowledging the significance of TGF-β1 not only on collagen type I [51] synthesis but also on pulp regeneration in general [50, 52, 53], we could show that moderate to highly concentrated Biodentine ® suspensions reduced the initial TGF-β1 secretion. However, low Biodentine ® concentrations seem to induce TGF-β1 secretion as well as other growth factors [50, 54–56]. In our study serial dilutions of the highest Biodentine ® suspension substantiated the concentration-dependent effects on collagen type I synthesis and TGF-β1 secretion.…”

Section: Discussionsupporting

confidence: 68%

Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

et al. 2016

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Preserving a patient’s own teeth—even in a difficult situation—is nowadays preferable to surgical intervention and therefore promotes development of suitable dental repair materials. Biodentine®, a mineral trioxide aggregate substitute, has been used to replace dentine in a bioactive and biocompatible manner in both the dental crown and the root. The aim of our study was to evaluate the influence of Biodentine® on pulp fibroblasts in vitro. For this study, one to five Biodentine® discs with a diameter of 5.1mm were incubated in DMEM. To obtain Biodentine® suspensions the media were collected and replaced with fresh medium every 24h for 4 days. Primary pulp cells were isolated from freshly extracted wisdom teeth of 20–23 year old patients and incubated with the Biodentine® suspensions. Proliferation, cell morphology, cell integrity and cell viability were monitored. To evaluate the effect of Biodentine® on collagen type I synthesis, the secretion of the N-terminal domain of pro-collagen type I (P1NP) and the release of transforming growth factor-β1 (TGF-β1) were quantified. None of the Biodentine® suspensions tested influenced cell morphology, proliferation or cell integrity. The cell viability varied slightly depending on the suspension used. However, the concentrations of P1NP of all pulp fibroblast cultures treated for 24h with the moderate to high Biodentine® concentration containing suspensions of day 1 were reduced to 5% of the control. Furthermore, a significant TGF-β1 reduction was observed after treatment with these suspensions. It could be shown that Biodentine® is biocompatible. However, dissolved particles of the moderate to high concentrated Biodentine® suspensions 24h after mixing induce a significant reduction of TGF-β1 release and reduce the secretion of collagen type I of primary pulp fibroblasts.

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“…, Peters et al . ). In the present study, h DPSCs were exposed to various cement extracts (undiluted, 1/2, 1/4,) in order to simulate the clinical conditions where vital pulp therapy is performed.…”

Section: Discussionmentioning

confidence: 97%

Comparative analysis of the biological effects of the endodontic bioactive cements MTA‐Angelus, MTA Repair HP and NeoMTA Plus on human dental pulp stem cells

et al. 2017

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Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla

Abstract: Both MTA and BD stimulated the expression of angiogenic genes and release of VEGF, inducing similar expression patterns; however, they appeared to inhibit the expression of specific genes, including ANGPT1 and FGF2.

Cited by 21 publications

References 29 publications

Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

Comparative analysis of the biological effects of the endodontic bioactive cements MTA‐Angelus, MTA Repair HP and NeoMTA Plus on human dental pulp stem cells

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