Effects of Two Saponins Extracted from the Polygonatum Zanlanscianense Pamp on the Human Leukemia (HL-60) Cells.

Wang, Zhao; Zhou, Jiangbing; Ju, Yi‐Hsu; Zhang, Hongjun; Liu, Mingjie; Xue, Li

doi:10.1248/bpb.24.159

Cited by 109 publications

(60 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…9) Dioscin, which was extracted from Polygonatum Zanlanscianense Pamp, has recently been reported to induce differentiation and apoptosis in the human leukemic HL60 cell line. 10) This study demonstrated for the first time the apoptotic potential of Dioscin in Hela cells where caspases-9 and caspase-3 were activated, suggesting that Dioscin induced apoptosis of Hela cells through the mitochondrial pathway. Cell Culture Hela (human cervical cancer cell line) was provided by the Cell Biology Laboratory of Tsinghua University.…”

mentioning

confidence: 65%

“…Earlier results showed that HL-60 cells underwent differentiation and apoptosis after Dioscin treatment. 10) This study detected the apoptotic effect of Dioscin on Hela cells and studied the pathway involved in apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Intracellular Staining of Bcl-2 Hela cells were treated with 2, 5 and 8 mM Dioscin for 18 h. 10 6 cells of each sample were collected and washed in PBS. Then, cells were fixed in 0.4% paraformaldehyde in PBS for 5 min.…”

Section: Chemicalsmentioning

confidence: 99%

See 2 more Smart Citations

Apoptosis Induced by Dioscin in Hela Cells.

Cai

Liu

Zhao

et al. 2002

Biological & Pharmaceutical Bulletin

Self Cite

103

View full text Add to dashboard Cite

Dioscin, a saponin extracted from the root of Polygonatum Zanlanscianense Pamp, markedly inhibited proliferation of Hela cells. The results indicated that Hela cells underwent apoptosis in dose-and time-dependent manners when treated with Dioscin. Caspase-3, -8 and -9 activities were also detected. The low enzymatic activity of caspase-8 and high activity of caspase-9 showed that the mitochondrial pathway was activated in apoptosis. The reduced expression of the survival protein Bcl-2 also confirmed this result. These studies may be significant in finding a new drug to treat human cervical cancer.

show abstract

mentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Apoptosis Induced by Dioscin in Hela Cells.

Cai

Liu

Zhao

et al. 2002

Biological & Pharmaceutical Bulletin

Self Cite

103

View full text Add to dashboard Cite

show abstract

“…1) influence biological responses (25,26). Wang et al (27) (1->4))), and methyl protodioscin, a furostanol with 26-O-glycopyranoside. These authors showed that only dioscin induces apoptosis although these .05 (Fisher's PLSD test) were considered to indicate significant differences compared to untreated cell control or hecogenin or tigogenin alone respectively.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of human rheumatoid arthritis synovial cell survival by hecogenin and tigogenin is associated with increased apoptosis, p38 mitogen-activated protein kinase activity and upregulation of cyclooxygenase-2

Liagre¹,

Vergne-Salle²,

Léger³

et al. 2007

Int J Mol Med

View full text Add to dashboard Cite

Abstract. We conducted our study to assess the antiproliferative and proapoptotic potential of hecogenin and tigogenin, two saponins which are structurally similar to diosgenin. We particularly focused our attention on mitogenactivated protein kinase (MAPK) activation in relation to apoptosis but also with the COX-2 expression and activity. Rheumatoid arthritis (RA) synoviocytes were isolated from fresh synovial biopsies obtained from five RA patients undergoing hip arthroplasty. Measurement of cell proliferation was determined using the MTT assay. Apoptosis was evaluated by studying caspase-8, caspase-9 and caspase-3 activities but also by quantification of DNA fragmentation. Quantification of human phospho-MAPKs was realized by ELISA. COX-2 expression was demonstrated by Western blot analysis and COX-2 activity by assay of endogenous prostaglandin E 2 (PGE 2 ) production. Tigogenin was more effective than hecogenin in inducing apoptosis in human RA fibroblast-like synoviocytes (FLS) which was caspase dependent but poly(ADP-ribose) polymerase independent and characterized by DNA fragmentation. Our results demonstrated hecogenin-and tigogenin-induced apoptosis through activation of p38 without affecting the JNK and ERK pathways. Indeed, pretreatment with a p38 inhibitor decreased saponin-induced apoptosis with a significant decrease in DNA fragmentation. Furthermore, the rate of apoptosis induced by hecogenin or tigogenin was associated with overexpression of COX-2 correlated with overproduction of endogenous PGE 2 . These new results provide strong evidence that a family of structurally similar plant steroids is capable of inducing apoptosis in human RA FLS with different rates and different signalling pathways. This study also confirms the discussed appearance of the downregulation or upregulation of COX-2 in cell apoptosis as a function of cell type. IntroductionPlant steroids have been thoroughly described for their pharmacological properties, including hypocholesterolemic (1), antidiabetic (2) and antioxidant activities (3,4). Particular attention has been given to their potential for cancer chemoprevention, especially as apoptosis inducers (5-10).Apoptosis is considered to be one of the mechanisms regulating autoimmune diseases such as rheumatoid arthritis (RA) (11). In the pathogenesis of RA, it is thought that the normal balance between proliferation and apoptosis of synovial fibroblasts is lost, leading to hyperplasia of these fibroblasts (12). Activated synovial cells cause growth of synovium in the articular cavity along with angiogenesis, invade the adjacent bone, promote production of inflammatory mediators by inflammatory cells, and cause cartilage and bone destruction (13). Therefore, it has been shown that stimulation of apoptosis in synovial fibroblasts might be useful for the treatment of RA (14,15).We showed for the first time that a plant steroid, diosgenin, inhibited the growth of human RA fibroblast-like synoviocytes (FLS) with apoptosis induction characterized by a loss of mitochondrial me...

show abstract

“…Numerous reports highlight the highly cytotoxic and anti-tumor properties of many saponins (Hostettmann and Marston, 1995). Much research indicates that extracts and steroidal saponins of Liliaceae plants have significant effects and potentially are anticancer drugs (Mimaki et al, 1995;Shao et al, 1997;Woo et al, 1998;Mimaki et al, 2001;Wang et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity of Paris quadrifolia Extract and Isolated Saponin Fractions Against Human Tumor Cell Lines

Stefanowicz–Hajduk¹,

Kawiak²,

Gajdus³

et al. 2011

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

Steroidal saponins isolated from many plant species belonging to Monocotyledones display potent cytotoxic activity towards many human tumor cells. We examined the cytotoxic effects of crude Paris quadrifolia extract for the first time, testing isolated saponin-rich fractions against four different human cell lines using the [(3-(4,5-dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide (MTT) assay. Cytotoxic activity was tested against human promyelocytic leukemia (HL-60) cells, human cervical adenocarcinoma (HeLa) cells and human breast cancer (MDA-MB-468) cells. Human skin fibroblasts were used as non-neoplastic control cells. Our results show significant activity of the weakly water-soluble solid residue and butanolic fraction against HL-60 and HeLa cells. The solid residue exerted cytotoxicity against all tested cell lines.K Ke ey y w wo or rd ds s: : In vitro cytotoxicity, MTT assay, Paris quadrifolia extract, steroidal saponins, human tumor cell lines.

show abstract

Effects of Two Saponins Extracted from the Polygonatum Zanlanscianense Pamp on the Human Leukemia (HL-60) Cells.

Cited by 109 publications

References 9 publications

Apoptosis Induced by Dioscin in Hela Cells.

Apoptosis Induced by Dioscin in Hela Cells.

Inhibition of human rheumatoid arthritis synovial cell survival by hecogenin and tigogenin is associated with increased apoptosis, p38 mitogen-activated protein kinase activity and upregulation of cyclooxygenase-2

Cytotoxic Activity of Paris quadrifolia Extract and Isolated Saponin Fractions Against Human Tumor Cell Lines

Contact Info

Product

Resources

About