Effects of Tylosin and Nisin on Canned Food Spoilage Bacteria

103

Nisin, an antibiotic produced by certain strains of Streptococcus lactis, is effective in preventing the outgrowth of Clostridium botuhum spores. Type A C. botulinurn spores were the most resistant to the inhibitory action of nisin requiring 1000-2000 I.U. of nisin/ ml for a 50% inhibition of outgrowth on TPYG agar plates. Type E spores were more sensitive requiring only 50-100 I.U./ml for 50% inhibition of outgrowth on TPYG agar plates. Type B spores displayed an intermediate level of sensitivity requiring 500-1000 I.U. of nisin/ml for 50% inhibition of outgrowth on TPYG agar plates. Similar levels of nisin were necessary to prevent spore outgrowth ln TPYG broth and BHI broth over a 7day incubation period. With prolonged incubation periods of up to 65 days in TPYG broth, spore outgrowth was observed sporadically at higher nisin levels with the type A and B spores which may indicate some decomposition of nisin with storage.

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Nisin on the Outgrowth of Clostridium botulinum Spores

Scott

Taylor

1981

103

“…The destruction of spore-forming bacteria by Tylosin has been reported by Denny et al (1961) and by Greenberg and Silliker (1961).…”

mentioning

confidence: 57%

The Effect of Tylosin on Coagulase‐Positive Staphylococci in Food Products

Greenberg

Silliker

1962

The ability of Tylosin to prevent the growth of coagulase-positive staphylococci was examined in four food substrates.Staphylococcal development was prevented during the organoleptically acceptable shelf life of ice cream mix by 20 ppm of the antibiotic.Staphylococciwere controlled in processed cheddar cheese spread, ham, and domestic sausage by 2.5, 3.0, and 5 ppm Tylosin, respectively.The possible use of Tylosin as a preventive of staphylococcal food poisoning in foods is discussed.

“…Tylosin is a new antimicrobial agent that has been described previously (Hamill et al, 1961;McGuire et al, 1961). An earlier observation by Malin (1958) of the destruction of sporeforming bacteria by tylosin has been confirmed by Denny et al (1961b) and by Greenberg and Silliker (1962). There is disagreement on the mode of action of tylosin.…”

Section: Introductionmentioning

confidence: 71%

Some Aspects of the Action of Tylosin on Clostridium Species PA 3679

Poole

Malin

1964

Spores of PA 3679 were derived from vegetative cells made resistant to an initial 4.0 ppm tylosin.Rate of germination of PA 3679 spores, either from the original sensitive stock or from a resistant stock, is not affected by tylosin. Vegetative cells of PA 3679 arising from germination and subsequent outgrowth of the resistant cells are killed by 0.8-0.9 ppm of residual tylosin. This is to he compared with 0.05-0.25 ppm initial tylosin required to kill sensitive cells. The residual level of tylosin in sterile spore-recovery media is dependent on the initial tylosin concentration and the medium.