Effects of ultrafine petrol exhaust particles on cytotoxicity, oxidative stress generation, DNA damage and inflammation in human A549 lung cells and murine RAW 264.7 macrophages

Mohan, Durga; Nathiya, S.; Rajasekar, Arvindganth; Devasena, T

doi:10.1016/j.etap.2014.08.003

Cited by 59 publications

(39 citation statements)

References 65 publications

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“…In general the results ofour present study demonstrates that exposure to DENPs induces significant cardiac toxicity in rats and this is evident from (a) reduction in total body weight (b) increase in levels of proinflammatory cytokines in plasma, (c) decrease in the levels of platelets in blood, (d) increase in the serum level 8-OhdG which is biomarker of oxidative DNA damage and(e) histologically result revealed degeneration and inflammatory cell infiltration of the cardiac myocytes with apparent separation of its fibers. Our previous characterization studies revealed that the DENPs contained majorly nanosize particles of size less than 100nm [7]. These airborne nanoparticles are of great concern as there is evident data suggesting that nanoparticles penetrate the lungs, resulting in inflammation and also enter other extra-pulmonary organs via, the circulation.…”

Section: Discussionmentioning

confidence: 99%

“…2-Collection and characterization of DENPs Petrol engines and duty light multi-cylinder diesel operating at a speed of 1500rpm in orderto collect DEPs, as the method by Durga et al, [7]. They were suspended in sterile normal saline (NaCl 0.9%) containing tween 80 (0.01%)to decrease aggregation, Control group received only normal saline with 0.01% tween 80 [8].The collected particles were less than 2.5μm in size.…”

Section: Chemicalsmentioning

confidence: 99%

“…They mainly contain nano-size particles that can easily enter the lungs [7]. Studies on humans reported that exposure to DEP in a controlled manner resulted in elevated thrombus formation and endothelial dysfunction.…”

Section: Introductionmentioning

confidence: 99%

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The possible protective role of quercetin on induced cardiac Oxidative DNA Damage by repeated exposure to diesel exhaust nanoparticles in rats (a histological and immunohistochemical study)

Faruk¹,

Mansy²,

ALasmari³

et al. 2018

J Histol Histopathol

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Diesel exhaust nanoparticles (DENPs) are gaseous exhaust and one component of particulate matter (PM) air pollution which is found to cause health hazards in humans. Quercetin is widely known for its antioxidant and antitoxic potential. We aimed todetect the protective effect of quercetin in DENP-induced cardio toxicity. Fifty Malealbino rats were divided into two groups,first group (ten rats) divided into two equal subgroups (one receive saline act as control and other receive quercetin,the second group (forty rats)divided equally into two subgroups (one was treated with repeated doses of DENPs [90µg/ rat and 180µg/ rat for 6 days intratracheally every two days] and other treated orally with quercetin (60 mg/kg B.Wt.) 1h prior to DENP exposure,DNA damagewas examined by measureserum8-hydroxydeoguanosine (8-OHdG) levels followed by histological and immunohistochemical studies for nuclear factor-kappa B(NF-κB) expression in heart tissues. Tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6) and serum level 8-Ohd Gare significantly higher in repeated doses of DENPs than the control group. Pretreatment with quercetin reduced significantly serum level 8-OhdGwhen compared to DENPs group.Cardiac myocytes disruption, vacuolation, inflammation and wide separation of its fibers in contrast to control group. Myocyteinflammations were significantly decreased with quercetin group compared with control group (decrease NF-κBimmunostaining). The present work yields experimentalevidence that quercetin can reduce oxidative DNA damage and inflammation induced by DENPs, so suggested that quercetin possible cardioprotective effect against DENPs.

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Section: Discussionmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

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The possible protective role of quercetin on induced cardiac Oxidative DNA Damage by repeated exposure to diesel exhaust nanoparticles in rats (a histological and immunohistochemical study)

Faruk¹,

Mansy²,

ALasmari³

et al. 2018

J Histol Histopathol

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“…The increase in the activity of these enzymes and also molecular expression enhance the capacity of an organism to manage oxidative stress and thus improves life expectancy [30,31]. Interestingly, due to tap stress, no significant changes in the activity of SOD and catalase enzyme have been observed in flies with or without any diet supplement of test compounds.…”

Section: Discussionmentioning

confidence: 94%

Effect of Forced Exercise (Tap Stress) on Drosophila Melanogaster and Efficacy of Abhrak Bhasma as Dietary Supplement.

Subedi¹,

Vartak

Kale³

2018

Asian J Pharm Clin Res

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Objective: Generation of reactive oxygen species and other oxidants as an outcome of aerobic respiration has been well documented. During boosted muscular activities/exercise, there is an increase in the rate of respiration and subsequent generation of oxidants; for which, organisms possess various defense systems. There is, however, paucity of reports on the effect of forced exercise in Drosophila melanogaster. Methods:The present investigation reports a method for inducing forced exercise, the "tap stress," in D. melanogaster. This method takes advantage of the natural tendency of Drosophila flies to climb/fly upward in a cylindrical tube. Simplicity of assembly, ease of experimentation and cost effectiveness are some of the key advantages of this method. Tap stress has been assumed to manifest through the generation of oxidative stress, thus, parameters indicative of oxidative stress have been evaluated. Results:The results indicate that tap stress induces oxidative stress mainly by influencing the non-catalytic components; total reduced glutathione (GSH), GSH: oxidized glutathione ratio, and free radical scavenging capacity. The catalytic components (superoxide dismutase, and catalase), on the contrary, remain largely unaffected. Abhrak bhasma (AB) influences total GSH content with an increase by about 38±21%, while flies on control diet register a decrease in total GSH content by 34±7% during tap stress. Feeding flies on AB, specifically, show a prominent increase in the transcription levels of cap "n" collar C, heat shock protein 70 and catalase genes (>1.5 fold). Conclusion:Overall, dietary supplement of AB and ascorbic acid benefits Drosophila flies in the management of oxidative stress induced through tap stress.

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“…Recent studies have shown that DEP exposure may help to generate reactive oxygen species (ROS) (Chao et al 2012;Vattanasit et al 2014) and decrease specific activities of superoxide dismutase (SOD), glutathione peroxidase (GSHPX) and glutathione reductase (GSHRX) in cells. DEP is also known to cause increases in lipid peroxidation, oxidative stress and DNA damage in human A549 lung epithelial cells and murine RAW 264.7 macrophages (Durga et al 2014). Because it is suspected that PP and PNMC induce toxicity potentially via oxidative damage (Aam & Fonnum 2007), this study was undertaken to investigate the effect of exposure to PP and PNMC on splenic lymphocytes and to evaluate the relationship between any PP-and PNMC-induced toxicities and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

In vitroeffect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production

Yang

Wan

et al. 2016

Journal of Immunotoxicology

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View Crossmark data Citing articles: 3 View citing articles JOURNAL OF IMMUNOTOXICOLOGY, 2016 VOL. 13, NO. 4, 548-556 http://dx.doi.org/10.3109/1547691X.2016 RESEARCH ARTICLEIn vitro effect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production Gasoline exhaust particles (GEP) and diesel exhaust particles (DEP) are considered to be some of the most important air pollutants. Among the many constituents in these pollutant particles, 4-pentylphenol (PP) and 3-methyl-4-nitrophenol (PNMC) are considered important phenolics in GEP and DEP, respectively. The aim of this study was to investigate the effect of in vitro exposure to commercially-supplied PP and PNMC on populations of, and production of interleukin (IL)-2, IL-4 and granzyme-B by, mouse splenic lymphocytes. After in vitro exposure to PP or PNMC for 48 h, splenocyte viability was measured, cell phenotypes, e.g. B-cell (CD19), T-cells (CD3), T-cell subsets (CD4 and CD8), were quantified by flow cytometry and production of IL-2, IL-4 and granzyme-B was assessed via ELISA. The oxidative toxicity of PP and PNMC toward the splenocytes was also evaluated using measures of hydroxyl radical and malondiadehyde production and changes in glutathione peroxidase and superoxide dismutase activities. Results showed that in vitro exposure to PP and PNMC inhibited splenic cell parameters in a dose-related manner. Exposure to PP and PNMC decreased splenic T-lymphocyte populations and splenocyte production of cytokines and granzyme B, as well as induced oxidative stress in the splenocytes. The results also showed that the percentages of CD3 + T-cells overall and of CD4 + and CD8 + T-cells therein, among exposed splenocytes, were reduced; neither compound appeared to affect levels of CD19 + B-cells. Overall, the suppressive effects of PP were stronger than PNMC. The data here provide support for the proposal that PP-/PNMC-induced toxicity in splenocytes may be due at least in part to oxidative damage and that PP and PNMC -as components of GEP and DEP -might significantly impact on splenic T-cell formation/release of cytokines/granzymes in situ.ARTICLE HISTORY

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Effects of ultrafine petrol exhaust particles on cytotoxicity, oxidative stress generation, DNA damage and inflammation in human A549 lung cells and murine RAW 264.7 macrophages

Cited by 59 publications

References 65 publications

The possible protective role of quercetin on induced cardiac Oxidative DNA Damage by repeated exposure to diesel exhaust nanoparticles in rats (a histological and immunohistochemical study)

The possible protective role of quercetin on induced cardiac Oxidative DNA Damage by repeated exposure to diesel exhaust nanoparticles in rats (a histological and immunohistochemical study)

Effect of Forced Exercise (Tap Stress) on Drosophila Melanogaster and Efficacy of Abhrak Bhasma as Dietary Supplement.

In vitroeffect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production

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