Effects of various levels of rosemary and oregano volatile oil mixture on oxidative stress parameters in quails

Abstract

The aim of this study was to determine the possible effects of various levels of rosemary and oregano volatile oil mixture dietary supplementation on oxidative status parameters in the blood and various organs of quails. A total of 880 one-day-old Pharaoh quails (Coturnix coturnix Pharaoh) including both males and females, were divided into four groups containing 220 quails and treated as follows: a control group with 0 mg volatile oil (VO)/kg of diet; group I, with 100 mg/kg rosemary VO (RVO) plus 100 mg/kg oregano VO (OVO, 50:50%); group II, 60 mg/kg rosemary VO plus 140 mg/kg oregano VO (30:70%); and group III, 140 mg/kg rosemary VO plus 60 mg/kg oregano VO (70:30%). The diets were prepared fresh for each treatment. The experiment was carried out for 42 days. The quails were euthanized and then serum, erythrocyte, heart, liver and spleen were obtained. Serum malondialdehyde (MDA), nitric oxide (NO), antioxidant activity (AOA), glutathione (GSH), vitamin A (VA), vitamin C (VC), erythrocyte superoxide dismutase (SOD), as well as MDA, NO and SOD levels from the heart, liver and spleen were determined by enzyme-linked immunosorbent assay (ELISA). The results of the study show that whereas the lowest serum MDA and serum NO values were observed in group III (p< 0.05), the highest serum MDA and serum NO values were in group I (p<0.05). Moreover, the highest NO and SOD levels in group I were found in the liver and spleen, respectively (p<0.05). In conclusion, supplementation with a rosemary and oregano VO mixture to the diets of quails may alter the antioxidant activity depending on the diets, and the most effective doses of rosemary and oregano VO mixture were 70 and 30%, respectively.

Effect of Hempseed (Cannabis sativa sp.) Inclusion to the Diet on Performance, Carcass and Antioxidative Activity in Japanese Quail (Coturnix coturnix japonica)

“…Hu et al ., (2008) reported that serum MDA levels were decreased in test rats those fed by diets with hemp seed oil at the end of 90-day trial period compared with control groups of contained soybean oil and the normal diet. Also, it is reported that extract of cinnamon ( Faix et al ., 2009 ; Ciftci et al ., 2010 ), rosemary ( Yesilbag et al ., 2011 ), oregano ( Botsoglou et al ., 2002 ) decreased MDA levels in broilers and quails ( Bulbul et al ., 2012 ).…”

Rosmarinus officinalis (Rosemary): A Novel Therapeutic Agent for Antioxidant, Antimicrobial, Anticancer, Antidiabetic, Antidepressant, Neuroprotective, Anti-Inflammatory and Anti-Obesity Treatment

“…For example, rosemary essential oil and extract have been shown to destroy and prevent free radicals [14,15]. Rosemary is also capable of preventing lipid peroxidation, a destructive process that is caused by oxidative stress [7,16]. In addition to reducing the amount of reactive species in the body, rosemary has been found to increase the activity of antioxidant enzymes [17].…”

Phytochemicals of ethanolic extract and essential oil ofPersicaria hydropiperand their potential as antibacterial agents for food packaging polylactic acid film

“…Food safety in terms of contamination and spoilage by microorganisms is a big issue and has always been a concern to consumers and food industries. In order to improve food safety, synthetic additives and preservatives which have harmful effects to human body (Bulbul, Bulbul, Biricik, Yesilbag, & Gezen, 2012) have been used. The limited storage life of foods and their association in foodborne disease outbreaks has resulted in commercial pressures to use synthetic chemicals as preservatives.…”