Effects of vinblastine on healing in microvascular anastomosis

Abstract

Reconstructive microsurgical procedures are getting more common in cancer patients. Adjuvant therapies, such as chemotherapy, radiotherapy, and immune therapy, are usually combined with surgical approaches to improve outcomes. This study was designed to investigate the effects of preoperative chemotherapy on healing in microvascular anastomosis. As a commonly preferred drug for neoadjuvant cancer treatment, vinblastine was used on rats in our study. Ninety-three Sprague-Dawley rats were used and randomly allocated into two groups as 72 experimental and 21 control rats. Vinblastine 2 mg/kg was administered as a single dose intraperitoneally on day 0 in the experimental group. Leukocytes and erythrocytes were counted on days 0, 1, 3, 5, 7, 10, and 15 in 6 rats of the experimental and 6 rats of the control group. We found that the neutropenia period ended on approximately day 7. After applying the medication, the experimental group (n = 72) was divided into three main groups (n = 24). We performed end-to-end femoral artery anastomosis on days 7 or 14 or 21, respectively, in each group. Each main group was divided into three subgroups (n = 8), and then we tested patency and took biopsies on days 7 or 14 or 21, respectively, in each subgroup. Histopathologic evaluation was carried out. The comparison of patency tests and pathologic examination indicated that there was no statistically significant difference between the two groups.

Antithrombotic effect of epigallocatechin gallate on the patency of arterial microvascular anastomoses

“…In addition to surgical and technical factors, the success of microsurgery is closely related to patients' health status, health problems, and medications. Several studies have examined the effects of various substances, including low-molecular-weight heparin, steroids, sildenafil, and vinblastine, on enhancing microvascular anastomosis patency [1][2][3][4][5][6]. However, the benefits of these substances have not been proven.…”

“…The patency of microvascular anastomoses is adversely affected by local and systemic factors [1]. Impaired intimal recovery and endothelial mechanisms promoting thrombus formation at the anastomotic site are common causes of decreased anastomosis Antithrombotic effect of epigallocatechin gallate on the patency of arterial microvascular anastomoses patency [1][2][3][4].…”

“…The patency of microvascular anastomoses is adversely affected by local and systemic factors [1]. Impaired intimal recovery and endothelial mechanisms promoting thrombus formation at the anastomotic site are common causes of decreased anastomosis Antithrombotic effect of epigallocatechin gallate on the patency of arterial microvascular anastomoses patency [1][2][3][4]. Dextran, heparin, low-molecular-weight heparin, and steroids are commonly administered in clinical practice to prevent thrombus formation.…”

“…Dextran, heparin, low-molecular-weight heparin, and steroids are commonly administered in clinical practice to prevent thrombus formation. Several drugs, including vinblastine, sildenafil, and botulinum toxin, have been used to increase anastomosis patency in experimental studies; however, the clinical applications of these drugs are currently limited [1,2,5,6].…”