Effects of whole seed priming on the in vitro germination of hybrid banana embryos (Musa spp.)

Arun, K.; Uma, S.; Saraswathi,; Backiyarani, S.; Durai, P.

doi:10.15258/sst.2013.41.3.10

Cited by 14 publications

(9 citation statements)

References 31 publications

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“…Prior to embryo rescue, the seeds were soaked in 96% ethanol for a minute and in 20% hypochlorite for 20 min, washed in sterile water, soaked in 10% hypochlorite for 10 min and finally washed in sterile water. Subsequently, we performed a priming by soaking the seeds in 10 ppm gibberellic acid (GA3) for three days to initiate shooting of the embryo (Arun et al, 2013 ). The embryos were taken out of the seeds under sterile conditions, transferred to Murashige & Skoog medium (MS) (Murashige & Skoog, 1962 ) with 2 ppm benzylaminopurine (BAP), 1 ppm biotin, 0.1 g/l myo-inositol and 3 g/l gelrite before autoclaving and placed in the dark to induce root elongation.…”

Section: Methodsmentioning

confidence: 99%

Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

et al. 2020

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Banana is an important fruit and food crop, but is threatened by Fusarium wilt, one of the most devastating soil-borne fungal diseases. Only host resistance facilitates banana cultivation in infested soils around the world, but the genetic basis of Fusarium wilt of banana (FWB) is unknown. We selfed a heterozygous wild banana accession Musa acuminata ssp. malaccensis (Mam, AA, 2n = 22) to generate a mapping population and to investigate the inheritance of resistance to Race 1 and tropical race 4 (TR4) that cause FWB. Phenotyping (N = 217) revealed segregation for resistance, and genotyping by sequencing resulted in 2802 high-quality single-nucleotide polymorphic markers (SNPs) that were used for genetic mapping. Combined analyses of these data showed that a single dominant resistance locus controls resistance to Race 1 and maps near the distal part of chromosome 10. Recombinants, together with the position of the putative resistance gene, were further analysed using graphical genotyping, which retrieved markers flanking a 360 kb genetic region that associates with Race 1 resistance. The region contains 165 putative genes on the reference genome, including 19 leucine-rich repeat receptor-like kinase-like genes. At the same position and phase, we also identified a QTL for TR4 resistance, showing that the locus for resistance against Race 1 provided partial resistance to TR4. However, this effect was far less significant and hence not included in the mapping. These data support the breeding of new banana varieties with resistance to Fusarium wilt.

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Section: Methodsmentioning

confidence: 99%

Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

et al. 2020

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“…Seeds hydropriming treatments were carried out referring to Arun et al (2013) with a modification in soaking time, i.e. 0 (without soaking), 1, 4, and 7 Days at room temperature.…”

Section: Hydropriming Treatmentmentioning

confidence: 99%

“…In order the seed to germinate, the embryo has to contact water to be non-dormant. Besides, dry ripe banana seeds have a hard texture with an impermeable inner surface of the seed coat; thus, they mostly fail to germinate due to lack of water supply or exchange of gases (Arun et al, 2013). Rescuing the embryo seems to be the best way to overcome this obstacle, which has significantly enhanced germination compared to conventional sowing the seeds (Wilson & Tenkouano, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Hydropriming Improves Germination and Plant Recovery During Embryo Rescue of Wild Banana Musa acuminata var. tomentosa

et al. 2021

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Wild bananas are believed to have genes for resistance to biotic and abiotic stress in nature, making them potential genetic resources for creating superior varieties. Wild banana seeds, such as Musa acuminata var. tomentosa are generally difficult to germinate in vivo, so that in vitro embryo culture technique is needed. This study aimed to increase embryo germination and regeneration of wild banana M. acuminata var. tomentosa by soaking the seeds as hydropriming. The treatment comprised of soaking the seeds in sterile distilled water for four periods of time: 0 (control), 1, 4, and 7 days. A total of 45 embryos for each treatment were planted on petri dishes containing MS + 0.5 mg/L BA + 1 mg/L biotin + 1 mg/L proline. The results showed that hydropriming increased the rate of embryo germination and regeneration. Seeds soaked for 1, 4, and 7 days successfully resulted in embryo germination percentages of 87%, 62%, and 62%, respectively, while the control unsoaked seeds germinated with a lower percentage of 42%. One-day soaking treatment was the most optimal treatment to increase the rate of germination and regeneration as well as obtained the best vigor as demonstrated by the highest average height of plantlets, number of leaves, and roots than other treatments. Thus, 1-day seed hydropriming is the best treatment for embryo rescue and regeneration of wild banana M. acuminata var. tomentosa

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“…Thus the success of banana breeding program mainly depends on the evaluation of large number of heterogeneous population obtained from a cross combination. Swennen and Vuylsteke (1993) and Arun et al (2013) evidenced the enhanced germination efficiency of banana seeds up to 30% through embryo rescue and embryo culture which needs to be further enhanced for still attaining better success in banana breeding (Batte et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Multiple shoot induction in zygotic embryos: A strategy for acceleration of banana breeding

Backiyarani

Uma

Saranya

et al. 2021

Preprint

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The presence of residual female fertility in most of the parthenocarpic banana accessions, embryo culture technique encourages the banana breeder to develop new hybrids through conventional breeding. Desirable trait can be fixed in banana in the first generation of hybrid progenies, but their evaluation is a long process owing to non-availability of uniform suckers/planting material. The duration of the breeding cycle can be reduced by developing more number of plantlets per hybrid event under in vitro embryo culture. Thus efforts were taken to standardize the multiple shoot formation from single embryo through media manipulation and decortications of embryo culture derived single plants. Success on multiple shoot formation was achieved up to 75% and 95% in seeded accessions of M.acuminata and M.velutina embryos respectively. In general, enhanced regeneration efficiency and rate of multiple shoot formation were recorded in media with BAP concentration of 17.76µm. Single in vitro plantlets derived through embryo culture were decorticated to 3 cubic cm size that resulted in 100% multiple shoot formations. This technique has been implemented from the seeds obtained from controlled pollination (18 cross combinations) and open pollination (nine accessions) of various genomic groups (ABB, AAB, AA) and their genetic fidelity of multiple shoots were also confirmed through microsatellite markers. As this technique, i) enhances the regeneration of hybrid seeds; ii) overcomes the risk of field mortality of the hybrid progenies and iii) allows simultaneous evaluation for multiple traits, will accelerate the breeding program by reducing the time taken for the release of potential banana hybrids.

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Effects of whole seed priming on the in vitro germination of hybrid banana embryos (Musa spp.)

Cited by 14 publications

References 31 publications

Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

Genetic mapping of Fusarium wilt resistance in a wild banana Musa acuminata ssp. malaccensis accession

Hydropriming Improves Germination and Plant Recovery During Embryo Rescue of Wild Banana Musa acuminata var. tomentosa

Multiple shoot induction in zygotic embryos: A strategy for acceleration of banana breeding

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