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Introduction: This study was undertaken to assess the antimicrobial, antioxidant, anti-inflammatory and antimalarial potential of fruit extract of Ananas comosus Linn. Bromelain is the major protease enzyme present in the pineapple. Methods: Screening of compounds using fluorescence-based assay was performed for antimalarial activity by Trager and Jenson method. Ethanolic extract of ananas cosmosus showed good antimalarial activity (EC50 = 1.94 ug/ml) as compared to the standard drug primaquine (EC50 = 0.07 ug/ml). In the case of antibacterial activity, ethanolic extract of pineapple showed the good MIC value, i.e. S. aureus (MIC= 3.12 ug/ml), S. typhi (MIC = 3.12 ug/ml) as compared to the standard drug ofloxacin (MIC= 0.43 ug/ml). Results: In the case of antifungal activity extract showed the remarkable activity A. niger (MIC= 1.56 ug/ml) as compared to the standard drug fluconazole (MIC = 0.10 ug/ml). In the case of antioxidant activity, % inhibition was found i.e. 44.13 %, which is nearly equal to the standard drug 48 %. Ananas cosmosus showed excellent antioxidant potential.

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