Effets des levuresSaccharomyces boulardiisur les trophozoïtes d’Entamoeba histolytica in vitroet dans l'amibiase caecale du jeune rat

Abstract

Mémoire.Un modèle original d'amibiase caecale mis au point chez le raton a été utilisé pour étudier l'effet de la levure Saccharomyces boulardii sur le développement des lésions. Des ratons infectés par Entamoeba histolytica ont été traités par Saccharomyces boulardii ; chez les traités, le nombre de ratons malades est significativement moins élevé, la gravité de l'affection, appréciée par l'aspect macros copique du caecum et la présence d'amibes a été diminuée. Les lésions observées sont semblables à celles des animaux témoins, mais leur processus de cicatrisation est accéléré. Par ailleurs, Sac charomyces boulardii n'a pas d'action amoebicide in vitro. Summary: Effects of Saccharomyces boulardii yeast on trophozoites of Entamoeba histolytica in vitroand on cecal amebiasis in the young rat.Lyophilized and rehydrated Saccharomyces boulardii yeasts were administered to young rats previously inoculated into the cecum with Entamoeba histolytica. The numbers of diseased young rats and the severity of the infection, assessed from the appearance of the cecum and its content of mucus and amebae, were signifi cantly reduced by the treatment. The lesions observed resembled those seen in the untreated controls, although healing was faster in the treated animals. Saccharomyces boulardii had no intrinsic amebicidal action in vitro.

Saccharomyces boulardii in childhood

“…1, 2). In vivo studies have shown that S. boulardii reduces in rats significantly the number of E. histolytica and the severity of symptoms [74]. In mice, S. boulardii reduces the number of C. albicans 20 to 50 times.…”

“…Nevertheless, intestinal permeability to mannitol is reduced by 93% in the presence of S. boulardii. Castagliuolo et al demonstrated that the 54 kDa protease inhibits the binding of toxin A and B to their receptors on the membranes of the intestinal villi [25,26,74].…”

Mechanisms of Action of Biotherapeutic Agents

“…Earlier studies showed that treatment with S. boulardii decreased mortality and morbidity in young rats infected with E. histolytica (38). CDR Sprague-Dawley young rats were infected with 5 X 10 5 trophozoites of E. histolytica and given either 1.8 X 10 9 CFU / d of S. boulardii or saline for 4 d. Rats were sacrificed on the 5th d and autopsied.…”

Up-to-date clinical and experimental basis for the use of probiotics

“…O Lactobacillus acidophilus impede também a adesão de microorganismos patogênicos em cultura de células intestinais humanas 13 . O tratamento com Saccharomyces boulardii reduz a mortalidade de ratos jovens infectados com Entamoeba histolytica 14 . In vitro, o contato entre este protozoário e o Saccharomyces boulardii, suas membranas ou o sobrenadante de cultivo da levedura, reduz o número de trofozoitos capazes de aderir a eritrócitos 15 .…”