Efficacy of a Novel Herbal Formulation (F2) on the Management of Obesity: In Vitro and In Vivo Study

Abstract

Background. Currently, obesity and its comorbidities have become a serious threat to human health necessitating urgent development of safe and effective therapy for their management. Materials and Methods. In this research, a novel polyherbal formulation (F2) was prepared by mixing specific proportions of royal jelly and lemon juice with ethanol extracts of Orostachys japonicus, Rhus verniciflua, and Geranium thunbergii. The antioxidant activity was assessed using DPPH and ABTS assay methods. The antiobesity potential of the F2 was assessed in vitro using 3T3-L1 fibroblast and in vivo using a high-fat diet (HFD) fed C57BL/6J mice model. F2 was administered in mice at the dose of 23 mg/kg and 46 mg/kg, twice daily by oral gavage. A well-accepted antiobesity agent, Garcinia cambogia (GC), at 200 mg/kg was used as a positive control. Results. F2 was observed to exhibit synergistic antiadipogenic activity in 3T3-L1 cells. This inhibition was reinforced by the downregulation of specific adipogenic transcription factors. Furthermore, F2 was also found to reduce mice body weight gain, food efficiency ratio, fasting blood glucose level, fat deposition into the liver, and mass of white adipose tissue. F2 also played a role in the excretion of fat consumed by the mice. For most of the assays performed, the F2 (46 mg/kg) was comparable to the positive control GC (200 mg/kg). In addition, potential and synergistic antioxidant activity was observed on F2. Conclusion. The results revealed that the formulation F2 exhibited potential antiobesity activity through the inhibition of adipocyte differentiation, dietary fat absorption, and reduction of free fatty acids deposition in tissues.

Anti-Obesity Potential of Ponciri Fructus: Effects of Extracts, Fractions and Compounds on Adipogenesis in 3T3-L1 Preadipocytes

“…Herbs that have shown good anti-adipogenic activity in scientific evaluation are Piper nigrum [44], Camellia sinensis [45], Trigonella foenum-graecum [46], Zingiber officinale [47], Aegle marmelos [48], Sibiraea augustata [49] and Corni Fructus [50], etc. Formulations obtained from herbal mixtures, such as Triphala [51], Vrikshamla [52], Dohongsamul-tang [53], Samchulgeonbi-tang [54], SH21B [55], Oyaksungi-san [56] and formulation F2 [57], etc. have also proven their efficacy for both the treatment of obesity and anti-adipogenic potential in in-vitro and in-vivo experiments.…”

Anti-Obesity Effect of Hot Water Extract of Barley Sprout through the Inhibition of Adipocyte Differentiation and Growth

“…Upon administration of BSE, a decrease in weight gain and a decrease in FER (food efficiency ratio) were observed in a dose-dependent manner. FER is the ratio of increasing weight to food intake, and a decrease in FER means less weight gain compared to food intake [ 61 , 62 , 63 , 64 ]. As adipose tissue weight reduction, adipocyte growth inhibition through morphology, and differentiation inhibition through Western blotting were observed, it was confirmed that the anti-obesity effect of BSE was a factor in reducing FER.…”

18KHT01, a Potent Anti-Obesity Polyherbal Formulation

“…Adipocyte differentiation and measurement of lipid accumulation were done according to the method described in the previous study with slight modification (Pandeya et al, 2021). The 3T3-L1 pre-adipocytes were seeded at a density of 5.2 × 10 3 cells/well of 24 well-plates and 6 × 10 4 cells/well of 6 well plates in DMEM supplemented with 10% NCS media.…”

“…The epididymal white adipose tissue, liver, kidney, and spleen were isolated, weighed, and immediately fixed into 10% formalin with PBS for histological analysis. Hematoxylin and eosin (H&E) staining was done for histological analysis according to the method described in the previous report (Pandeya et al, 2021). Micrographs were analyzed using an EVOS XL core light microscope.…”