Efficacy of Alpinumisoflavone Isolated from Maclura tricuspidata Fruit in Tumor Necrosis Factor-α-Induced Damage of Human Dermal Fibroblasts

Abstract

The skin is an important organ in the human body that protects the body from environmentally hazardous substances. Reactive oxygen species (ROS) cause inflammatory reactions and degradation of the extracellular matrix leading to skin aging and various cutaneous lesions. This study evaluated the potential of isoflavones isolated from Maclura tricuspidata fruit to prevent TNF-α-induced skin inflammation in normal human dermal fibroblasts (HDFs). It focused on alpinumisoflavone (AIF) that suppressed the accumulation of ROS and nitric oxide (NO) in tumor necrosis factor-alpha (TNF-α)-treated HDFs. AIF inhibited the TNF-α-induced increase in matrix metalloproteinase-1, decreased procollagen I α1, and suppressed pro-inflammatory mediators and pro-inflammatory cytokines, including NO synthase, cyclooxygenase-2, interleukin (IL)-1β, IL-6, and IL-8 that trigger inflammatory responses. AIF inhibited nuclear factor-κB and activating protein 1 mitogen-activated protein kinases that were increased by TNF-α stimulation. These results suggest that AIF may protect skin from aging and various cutaneous lesions.

The Molecular Mechanism of Polyphenols with Anti-Aging Activity in Aged Human Dermal Fibroblasts

“…A tumor necrosis factor-α (TNF-α)-induced damaged fibroblast could also be alleviated by several polyphenols [ 48 , 49 , 50 , 51 ] ( Table 1 ). Alpinumisoflavone (AIF), (-)-catechin, epigallocatechin-3-gallate (EGCG), and 7,8-dihydroxyflavone (7,8-DHF) suppressed the TNF-α-induced MMP-1 synthesis and enhanced procollagen I [ 48 , 49 , 50 , 51 ]. AIF and (-)-catechin inhibited NF-κB activity and COX-2 [ 48 , 49 ].…”

“…Alpinumisoflavone (AIF), (-)-catechin, epigallocatechin-3-gallate (EGCG), and 7,8-dihydroxyflavone (7,8-DHF) suppressed the TNF-α-induced MMP-1 synthesis and enhanced procollagen I [ 48 , 49 , 50 , 51 ]. AIF and (-)-catechin inhibited NF-κB activity and COX-2 [ 48 , 49 ]. 7,8-DHF also significantly upregulated the expression of antioxidant enzymes including manganese superoxide dismutase (Mn-SOD), catalase, and heme oxygenase-1 (HO-1) [ 50 ].…”

Improvement of Damage in Human Dermal Fibroblasts by 3,5,7-Trimethoxyflavone from Black Ginger (Kaempferia parviflora)

“…The reaction was performed using the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems), and the thermal conditions were set to 40 cycles (95 °C for 15 s, 60 °C for 30 s, 95 °C for 30 s). The primer sequences are shown in a previous paper [ 40 ], and β-actin was used as the reference gene.…”

Protective Effect of the Pearl Extract from Pinctada fucata martensii Dunker on UV‐Induced Photoaging in Mice

“…Change of photoaged skin is closely related to degradation of collagen and elastic fibers in the dermis, [29] which is featured by sagging, wrinkling, coarseness, and erythema [30] . Photoaging stimulates the expression of intracellular ROS, which is a major part of the inflammatory response and ECM degradation that in turn leads to skin aging and various cutaneous lesions [31] …”

“…[30] Photoaging stimulates the expression of intracellular ROS, which is a major part of the inflammatory response and ECM degradation that in turn leads to skin aging and various cutaneous lesions. [31] The representative photographs of the UV-irradiated skin lesions at the ninth week were presented in Figure 2A, including skin appearance and skin pinch testing. Throughout the entire experiment, the dorsal skin in the SC group was healthy, suggesting that the shaving approach did not influence the skin morphology.…”