Efficacy of clarithromycin on biofilm formation of methicillin-resistant Staphylococcus pseudintermedius

DiCicco, Matthew; Neethirajan, Suresh; Singh, Ameet; Weese, J. Scott

doi:10.1186/1746-6148-8-225

Cited by 32 publications

(36 citation statements)

References 33 publications

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“…These data are comparable to data from other geographic areas (Dicicco et al 2012;Osland et al 2012;Singh et al 2013). In particular, this study showed that biofilm formation did not differ between MRSP and MSSP isolates, suggesting that the ability of dog-origin S. pseudintermedius to produce biofilms is widespread in this geographic area.…”

Section: Discussionsupporting

confidence: 89%

“…Among staphylococci, Staphylococcus epidermidis is widely known as the predominant cause of biofilminduced catheter-and medical device-related sepsis in humans (O'Gara & Humphreys 2001). Recently, several studies reported that MRSP, especially sequence type (ST) 71, also has an ability to produce biofilms, suggesting the necessity of investigation for the prevalence of biofilm-producing S. pseudintermedius in veterinary practices (Dicicco et al 2012;Osland et al 2012;Singh et al 2013). In South Korea, several studies have reported a high prevalence of MRSP in dogs Yoon et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Han

Yang

Park

2015

Veterinary Quarterly

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Background: Staphylococcus pseudintermedius is a common bacterial pathogen in dogs with pyoderma, otitis externa, and wound infection. Objective: The purpose of this study was to evaluate the biofilm-producing ability for methicillin-resistant (MRSP) and methicillin-susceptible (MSSP) S. pseudintermedius isolates from healthy dogs. Animals and methods: Ninety-two isolates, consisting of 40 MRSPs and 52 MSSPs, were included in the study. Biofilm production was determined using a quantitative adherence assay in 96-well tissue culture plates and polymerase chain reaction (PCR) targeting icaA and icaD genes. The prevalence of methicillin-resistance in the isolated S. pseudintermedius was calculated using standard equations. The presence of methicillin resistance (MR) and biofilm-producing ability between nasal-and ocular-origin S. pseudintermedius was compared using the chi-square (x 2 ) test. The associations between the degree of biofilm production and the presence of methicillin-resistance or sample origin were determined using Fisher's exact test. Results: All of the MRSP and MSSP isolates were able to produce biofilms, and 93.5% of the isolates were strong or moderate biofilm producers, regardless of the presence of methicillin-resistance or source. Methicillin-resistance was more prevalent in nasal isolates. There was no association between the presence of icaA/icaD and biofilm production. Conclusions: Findings from this study indicate a high prevalence of biofilm-producing S. pseudintermedius in healthy dogs in South Korea, which may have a significant influence to animal health.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Han

Yang

Park

2015

Veterinary Quarterly

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“…The MIC to act against microbial biofilm ranges from 10–100 times higher than that necessary against the planktonic form [34]. …”

Section: Resultsmentioning

confidence: 99%

Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species

Toledo

Ramos

Spósito

et al. 2016

IJMS

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Background: The incidence of fungal infections, especially those caused by Candida yeasts, has increased over the last two decades. However, the indicated therapy for fungal control has limitations. Hence, medicinal plants have emerged as an alternative in the search for new antifungal agents as they present compounds, such as essential oils, with important biological effects. Published data demonstrate important pharmacological properties of the essential oil of Cymbopogon nardus (L.) Rendle; these include anti-tumor, anti-nociceptive, and antibacterial activities, and so an investigation of this compound against pathogenic fungi is interesting. Objective: The aim of this study was to evaluate the chemical composition and biological potential of essential oil (EO) obtained from the leaves of C. nardus focusing on its antifungal profile against Candida species. Methods: The EO was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Testing of the antifungal potential against standard and clinical strains was performed by determining the minimal inhibitory concentration (MIC), time-kill, inhibition of Candida albicans hyphae growth, and inhibition of mature biofilms. Additionally, the cytotoxicity was investigated by the IC50 against HepG-2 (hepatic) and MRC-5 (fibroblast) cell lines. Results: According to the chemical analysis, the main compounds of the EO were the oxygen-containing monoterpenes: citronellal, geranial, geraniol, citronellol, and neral. The results showed important antifungal potential for all strains tested with MIC values ranging from 250 to 1000 μg/mL, except for two clinical isolates of C. tropicalis (MIC > 1000 μg/mL). The time-kill assay showed that the EO inhibited the growth of the yeast and inhibited hyphal formation of C. albicans strains at concentrations ranging from 15.8 to 1000 μg/mL. Inhibition of mature biofilms of strains of C. albicans, C. krusei and C. parapsilosis occurred at a concentration of 10× MIC. The values of the IC50 for the EO were 96.6 μg/mL (HepG-2) and 33.1 μg/mL (MRC-5). Conclusion: As a major virulence mechanism is attributed to these types of infections, the EO is a promising compound to inhibit Candida species, especially considering its action against biofilm.

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“…MRSP isolates are resistant to several clinically useful antibiotics including b-lactam agent such as clarithromycin (DiCicco et al 2012). Fosfomycin is a wide spectrum antibiotic used for treating urinary tract and genital tract infections caused by both gram-positive and gram-negative bacterial strains (Falagas et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Neethirajan

DiCicco

2013

Appl Nanosci

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The influence of fosfomycin on methicillinresistant Staphylococcus pseudintermedius (MRSP) as the target cell was studied by atomic force microscopy (AFM). Nanoscale images of the effects of the antibiotic fosfomycin on this gram-positive bacterium's cell were obtained in situ without fixing agents. Our study has demonstrated substantial morphological and topographical differences between the control and fosfomycin-treated MRSP cells. The AFM investigations further revealed the rough surface morphology and a 30 % shrinkage in size of the fosfomycin-treated cell and the leakage of cytoplasmic components from the cell. The damage of cell membrane integrity and the cell surface degradation as observed elaborates the antibacterial activity of fosfomycin. The AFM image analysis also reveals that the fosfomycin inhibits cell division, and prevents the adhesion on the surface discouraging the biofilm attachment. The microtitre plate assay results conform to the atomic force microscopy image analysis. This is the first visual demonstration of the effect of fosfomycin on MRSP cells.

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Efficacy of clarithromycin on biofilm formation of methicillin-resistant Staphylococcus pseudintermedius

Cited by 32 publications

References 33 publications

Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Emergence of biofilm-producingStaphylococcus pseudintermediusisolated from healthy dogs in South Korea

Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species

Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

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