Efficacy of different biorational insecticides against Aphis nerii Boyer de Fonscolombe (Hemiptera. Aphididae) on Gymnema sylvestre (R.Br) under laboratory and field conditions

Shivakumara, K. T.; Keerthi, M. C.; Polaiah, A. C.

doi:10.1016/j.jarmap.2021.100358

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Pesticides can be replaced by biopesticides [242]. Diverse biologically derived compounds have pesticidal activity against insect pests and diseases [243,244]. It is imperative to evaluate the amount, number of applications, and suitable delivery approaches of these potential BCAs in field conditions for the effective management of plant viral diseases.…”

Section: Future Prospectsmentioning

confidence: 99%

Mechanisms of Microbial Plant Protection and Control of Plant Viruses

Manjunatha

Rajashekara

Uppala

et al. 2022

Plants

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Plant viral diseases are major constraints causing significant yield losses worldwide in agricultural and horticultural crops. The commonly used methods cannot eliminate viral load in infected plants. Many unconventional methods are presently being employed to prevent viral infection; however, every time, these methods are not found promising. As a result, it is critical to identify the most promising and sustainable management strategies for economically important plant viral diseases. The genetic makeup of 90 percent of viral diseases constitutes a single-stranded RNA; the most promising way for management of any RNA viruses is through use ribonucleases. The scope of involving beneficial microbial organisms in the integrated management of viral diseases is of the utmost importance and is highly imperative. This review highlights the importance of prokaryotic plant growth-promoting rhizobacteria/endophytic bacteria, actinomycetes, and fungal organisms, as well as their possible mechanisms for suppressing viral infection in plants via cross-protection, ISR, and the accumulation of defensive enzymes, phenolic compounds, lipopeptides, protease, and RNase activity against plant virus infection.

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Section: Future Prospectsmentioning

confidence: 99%

Mechanisms of Microbial Plant Protection and Control of Plant Viruses

Manjunatha

Rajashekara

Uppala

et al. 2022

Plants

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“…The survival of spores on leaf surfaces until they encounter the host is ascribed to the leaf exposure approach, and the actual dose of conidia per insect is enhanced as the insect moves across the treated leaf surface ( Fernandez et al, 2001 ). Compared to the spray method (46.67%), the leaf treatment method (53.37%) had the highest mean cumulative mortality at 96 h. In contrast, spray method caused higher mortality in Aphis nerii (Boyer de Fonscolombe) as compared to the leaf dip method ( Shivakumara, Keerthi & Polaiah, 2022 ). This could be explained by the fact that moving on treated leaves while feeding is probably the most important means of contact leading to infection in case of contact pesticides.…”

Section: Discussionmentioning

confidence: 89%

Bioefficacy of engineered Beauveria bassiana with scorpion neurotoxin, LqqIT1 against cotton mealybug, Phenacoccus solenopsis and cowpea aphid, Aphis craccivora

Rajput,

Suroshe,

Yadav

et al. 2023

PeerJ

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Cotton mealybug, Phenacoccus solenopsis (Tinsley) and cowpea aphid Aphis craccivora (Koch) are notorious polyphagous, hemipteran sap sucking insect pests. A recombinant toxin gene ‘LqqIT1’ from the scorpion Leiurus quinquestriatus quinquestriatus (Ehrenberg) was cloned in the pAL1 fungal expression vector and then expressed in the entomopathogenic fungus Beauveria bassiana (Balasmo) using genetic modification techniques. The genetically transformed B. bassiana strain (BbLqqIT1-3) and its un-transformed parent strain (Bb-C) were screened to infect the third instar nymphs of P. solenopsis and first instar nymph of A. craccivora through leaf treatment and topical application (spray) method at 1 * 107 spores per ml concentration. The recombinant strain BbLqqIT1-3 was highly pathogenic against A. craccivora but non pathogenic to P. solenopsis. BbLqqIT1-3 induced 72 and 43.33% mortality in A. craccivora nymphs 96 h after leaf treatment and topical application, respectively. The nymphs of A. craccivora infected with BbLqqIT1-3 displayed classical neurotoxic symptoms such as sluggishness, solublize and liquification of the body. Crude soluble toxin protein, BbLqqIT1a-CSE and Bb-WT-CSE was extracted from the BbLqqIT1-3 and Bb-C, respectively using ammonium sulphate precipitation method, and their oral toxicity was analyzed at 5 µg/ml concentration. The survival of the studied insects was negatively affected by the crude soluble toxin extracts. The LT50 values of BbLqqIT1a-CSE against P. solenopsis and A. craccivora were 22.18 and 17.69 h, respectively. Exposure to crude soluble toxin extracts also accounted for the imbalance of ionic concentrations in the hemolymph of treated insects such as hyperpotassemia (3.53–8.18 meq/ml) in the P. solenopsis and hypopotassemia (7.52–0.47 meq/ml) in A. craccivora. The transformed fungus BbLqqIT1-3 strain exhibited promising results in invitro study.

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