Efficacy of FimA antibody and clindamycin in silkworm larvae stimulated with <i>Porphyromonas gulae</i>

Yoshida, Sho; Inaba, Hiroaki; Nomura, Ryota; Murakami, Masaru; Yasuda, Hidemi; Nakano, Kazuhiko; Matsumoto‐Nakano, Michiyo

doi:10.1080/20002297.2021.1914499

Cited by 4 publications

(2 citation statements)

References 52 publications

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“…However, 2.6% of P. gulae strains isolated from dogs with periodontitis and 13.3% of P. gulae strains isolated from dogs without periodontitis were resistant to metronidazole. In the study of Yoshida et al (2021), high doses of metronidazole were required to inhibit the growth of P. gulae.…”

Section: Discussionmentioning

confidence: 99%

Effect of spiramycin and metronidazole on canine dental biofilm bacteria

et al. 2022

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Periodontal diseases are the most common condition in companion animal practice. The administration of antibiotics is associated with the therapy of these diseases. The aim of the present study was to verify the effect of antibiotics on canine dental biofilm bacteria due to increasing antibiotic resistance. Dental biofilm samples were taken from six dogs before and after administration of antibiotics, specifically, the combination of spiramycin and metronidazole. The samples were cultured on solid media under aerobic and anaerobic conditions. Sequencing analysis of the 16S rRNA gene was used to identify bacterial isolates. In addition, total bacterial DNA was extracted from samples from one dog and the V3−V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq platform. Using cultivation, 55 isolates belonging to 4 phyla were isolated before antibiotics administration, and 36 isolates belonging to 3 phyla were isolated after antibiotics administration. A significant decrease was noted in the genera Porphyromonas, Neisseria and Frederiksenia, whereas there was a significant increase in the genus Streptococcus. Of the total microbiota, there were 69 operational taxonomic units (OTUs) belonging to 11 phyla before antibiotics administration, and 51 OTUs belonging to 8 phyla after antibiotics administration. A significant decrease was recorded in the genus Porphyromonas, while a significant increase in the genus Capnocytophaga. The significant effect of spiramycin and metronidazole on the genus Porphyromonas at the time of their administration was confirmed by both cultivation and amplicon sequencing.

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Section: Discussionmentioning

confidence: 99%

Effect of spiramycin and metronidazole on canine dental biofilm bacteria

et al. 2022

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“…These bind to host cell surface receptor proteins and participate in a variety of biological processes, including cross-membrane tracking, invasion, and migration [ 3 ]. They can be toxic to the host, causing inflammation [ 4 ]. Mannose supplementation or receptor inhibition may affect the adhesin–receptor interaction in certain adhesins, such as mannose, which is critical in immunological activation.…”

Section: Introductionmentioning

confidence: 99%

Glycan-Adhering Lectins and Experimental Evaluation of a Lectin FimH Inhibitor in Enterohemorrhagic Escherichia coli (EHEC) O157:H7 Strain EDL933

Park

Kim

Cho

2022

IJMS

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In this study, we tried to develop a FimH inhibitor that inhibits adhesion of enterohemorrhagic Escherichia coli (EHEC) on the epithelium of human intestine during the initial stage of infections. Using a T7 phage display method with a reference strain, EHEC EDL933, FimH was selected as an adherent lectin to GM1a and Gb3 glycans. In order to detect the ligand binding domain (LBD) of FimH, we used a docking simulation and found three binding site sequences of FimH, i.e., P1, P2, and P3. Among Gb3 mimic peptides, P2 was found to have the strongest binding strength. Moreover, in vitro treatment with peptide P2 inhibited binding activity in a concentration-dependent manner. Furthermore, we conducted confirmation experiments through several strains isolated from patients in Korea, EHEC NCCP15736, NCCP15737, and NCCP15739. In addition, we analyzed the evolutionary characteristics of the predicted FimH lectin-like adhesins to construct a lectin-glycan interaction (LGI). We selected 70 recently differentiated strains from the phylogenetic tree of 2240 strains with Shiga toxin in their genome. We can infer EHEC strains dynamically evolved but FimH was conserved during the evolution time according to the phylogenetic tree. Furthermore, FimH could be a reliable candidate of drug target in terms of evolution. We examined how pathogen lectins interact with host glycans early in infection in EDL933 as well as several field strains and confirmed that glycan-like peptides worked as an initial infection inhibitor.

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Molecular mechanism and potential application of bacterial infection in the silkworm, Bombyx mori

Wang

Sun

et al. 2022

Developmental & Comparative Immunology

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Efficacy of FimA antibody and clindamycin in silkworm larvae stimulated with Porphyromonas gulae

Cited by 4 publications

References 52 publications

Effect of spiramycin and metronidazole on canine dental biofilm bacteria

Effect of spiramycin and metronidazole on canine dental biofilm bacteria

Glycan-Adhering Lectins and Experimental Evaluation of a Lectin FimH Inhibitor in Enterohemorrhagic Escherichia coli (EHEC) O157:H7 Strain EDL933

Molecular mechanism and potential application of bacterial infection in the silkworm, Bombyx mori

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