Efficacy of immunization against bovine mastitis using a Staphylococcus aureus avirulent mutant vaccine

Pellegrino, Matías Santiago; Giraudo, José; Raspanti, Claudia; Odierno, Liliana; Bogni, Cristina

doi:10.1016/j.vaccine.2010.04.056

Cited by 29 publications

(28 citation statements)

References 26 publications

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“…In order to treat mastitis effectively and create prevention strategies etiological agents must be determined. Treatment of mastitis relies heavily on the use of antibiotics as the cause is mostly bacterial [4].…”

Section: Discussionmentioning

confidence: 99%

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Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

et al. 2015

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The aim of this study was to investigate the etiology of subclinical mastitis (SCM) in dairy Jersey cows with the use of bacteriological and molecular identifi cation methods. In the study 121 Jersey and 78 hybrid Jersey cows with SCM were observed in the Samsun district of Turkey. A total of 411 California mastitis test (CMT) positive milk samples from these animals were examined bacteriologically. The prevalence of subclinical mastitis was 54.75% and 67.2% in Jerseys and hybrids, respectively. On bacteriological examination, a total of 92 strains were isolated from 411 milk samples. The most prevalent bacteria were Staphylococcus spp. (69.56%). Among them 24 isolates were Staphylococcus aureus (26.08%) the other isolates were Streptococcus dysgalactiae (23.91%), Enterococcus spp. (3.26%) and Streptococcus agalactiae (3.26%). All strains were identifi ed with bacteriological culture methods, as well as by Polymerase Chain Reaction (PCR). Gram-negative bacteria were not isolated. In conclusion, the etiology of SCM in full blood and hybrid Jersey dairy cows in Samsun and the prevalence of bacteria were determined. The relatively high prevalence of SCM indicates the potential need for the consideration of some factors contributing to the formation of mastitis (e.g. management) as well as bacterial agents. The present study and further studies may be useful to develop mastitis vaccines by means of providing true vaccine strain sources.

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Section: Discussionmentioning

confidence: 99%

“…These economic losses are caused by reduced milk yield, discarded milk, replacement cost, extra labor, costs of treatment, veterinary care and culling [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

et al. 2015

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“…Several active and passive immunization strategies involving whole S. aureus cells of avirulent or mutant strains (Pellegrino et al, 2010), recombinant proteins (Uppalapati et al, 2014), mutated versions of toxin components (Spaulding et al, 2012;Gampfer et al, 2002a,b), native toxins (Spaulding et al, 2012) or intravenous immunoglobulin therapy (Darenberg et al, 2003(Darenberg et al, , 2004 and neutralizing antibodies (Varshney et al, 2011;Sully et al, 2014) have been studied to combat against S. aureus infections. Vaccines and antibodies targeting surface components such as capsular polysaccharides have been met with little to moderate success due their limited role in S. aureus virulence and pathogenesis mechanisms (O'Riordan and Lee, 2004;Schaffer and Lee, 2009).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of recombinant SEA-TSST fusion toxoid for protection against superantigen induced toxicity in mouse model

Reddy

Paul

Sripathy

et al. 2015

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“…aureus have been of great interest for a long time and have been studied for immunization of cows since the ‘80s [57]. Some teams have managed to produce attenuation by chemical mutagenesis [58] in order to elicit high specific humoral response in cows, but unfortunately this caused only a weak reduction in shedding of bacteria, and no difference in the reduction of somatic cell counts (SCC) in milk when vaccinated groups were challenged. Besides, the genetic basis for the attenuation of this strain was still unknown, which may be a concern considering the necessity to obtain a stable and safe vaccine.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a vraG Mutant in a Genetically Stable Staphylococcus aureus Small-Colony Variant and Preliminary Assessment for Use as a Live-Attenuated Vaccine against Intrammamary Infections

et al. 2016

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Staphylococcus aureus is a leading cause of bovine intramammary infections (IMIs) that can evolve into difficult-to-treat chronic mastitis. To date, no vaccine formulation has shown high protective efficacy against S. aureus IMI, partly because this bacterium can efficiently evade the immune system. For instance, S. aureus small colony variants (SCVs) have intracellular abilities and can persist without producing invasive infections. As a first step towards the development of a live vaccine, this study describes the elaboration of a novel attenuated mutant of S. aureus taking advantage of the SCV phenotype. A genetically stable SCV was created through the deletion of the hemB gene, impairing its ability to adapt and revert to the invasive phenotype. Further attenuation was obtained through inactivation of gene vraG (SACOL0720) which we previously showed to be important for full virulence during bovine IMIs. After infection of bovine mammary epithelial cells (MAC-T), the double mutant (ΔvraGΔhemB) was less internalized and caused less cell destruction than that seen with ΔhemB and ΔvraG, respectively. In a murine IMI model, the ΔvraGΔhemB mutant was strongly attenuated, with a reduction of viable counts of up to 5-log10 CFU/g of mammary gland when compared to the parental strain. A complete clearance of ΔvraGΔhemB from glands was observed whereas mortality rapidly (48h) occurred with the wild-type strain. Immunization of mice using subcutaneous injections of live ΔvraGΔhemB raised a strong immune response as judged by the high total IgG titers measured against bacterial cell extracts and by the high IgG2a/IgG1 ratio observed against the IsdH protein. Also, ΔvraGΔhemB had sufficient common features with bovine mastitis strains so that the antibody response also strongly recognized strains from a variety of mastitis associated spa types. This double mutant could serve as a live-attenuated component in vaccines to improve cell-mediated immune responses against S. aureus IMIs.

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Efficacy of immunization against bovine mastitis using a Staphylococcus aureus avirulent mutant vaccine

Cited by 29 publications

References 26 publications

Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

Investigation On The Etiology Of Subclinical Mastitis In Jersey And Hybrid Jersey Dairy Cows

Evaluation of recombinant SEA-TSST fusion toxoid for protection against superantigen induced toxicity in mouse model

Characterization of a vraG Mutant in a Genetically Stable Staphylococcus aureus Small-Colony Variant and Preliminary Assessment for Use as a Live-Attenuated Vaccine against Intrammamary Infections

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