Efficacy of intramuscular moxifloxacin in the treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus

Soranoglou, Vasileios; Galanopoulos, Ilias; Giamarellos‐Bourboulis, Evangelos J.; Papalois, Αpostolos; Poultsides, Lazaros; Choreftaki, Theodosia; Kanellakopoulou, Kyriaki

doi:10.1016/j.ijantimicag.2017.01.041

Cited by 12 publications

(10 citation statements)

References 30 publications

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“…RIF, when used alone, is susceptible to drug resistance; therefore, RIF in combination with other antibiotics to gradually treat osteomyelitis has become a trend for treating chronic osteomyelitis [42]. MOX exhibits a wide antibacterial spectrum and high bioavailability within the bone [22]. In our study, both quantitative and qualitative antibacterial tests demonstrated that the combined drug-loaded microspheres exhibited more effective bacteriostasis than single drug-loaded microspheres.…”

Section: Discussionmentioning

confidence: 58%

“…Moxifloxacin (MOX) is a fourth-generation quinolone and has excellent broad-spectrum antibacterial properties and low systemic toxicity; thus, it has been used for treating chronic osteomyelitis. In addition, previous studies have confirmed that compared with other antibiotics such as glycopeptides, clindamycin and RIF, MOX exhibits higher bioavailability and bone tissue penetration to enter bone cells and kill latent S. aureus [22].…”

mentioning

confidence: 84%

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Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres

Qiao

Yuan

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Osteomyelitis is difficult to treat because infective bone is poorly accessible for intravenously administering antibiotics and biofilm formation increases bacterial resistance. In this study, microspheres prepared using poly(lactide-co-glycolide) (PLGA) and embedded with moxifloxacin (MOX-PLGA microspheres) and rifampicin/moxifloxacin (RIF/MOX-PLGA microspheres) using the water-in-oil-inwater double emulsion solvent evaporation technique were used for local delivery. Shape of MOX-PLGA microspheres and RIF/MOX-PLGA microspheres were spherical, mean particle size of them were 20.52 lm and 16.62 lm, respectively. Encapsulation efficiency of the MOX-PLGA microspheres was 17.35% ± 2.42%. However, the encapsulation efficiency for MOX and RIF in RIF/MOX-PLGA microspheres was 33.25% ± 7.51% and 49.0% ± 11.25%, respectively. Moxifloxacin and rifampicin were released slowly from microspheres. Both microspheres can efficiently release antibiotics in vitro. Antibacterial and bacterial biofilm-inhibition properties of the released solution were investigated from RIF/MOX-PLGA, MOX-PLGA, and blank PLGA microspheres at varying time points in vitro. RIF/ MOX-PLGA microspheres demonstrated the strongest antibacterial activity and bacterial biofilm-inhibition property than the other two microspheres (p < .05). This study suggests that the novel RIF/ MOX-PLGA microspheres can be used as a promising carrier for osteomyelitis treatment.

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Section: Discussionmentioning

confidence: 58%

mentioning

confidence: 84%

Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres

Qiao

Yuan

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…e experimental model MRSA osteomyelitis in rabbits was used by Soranglou et al [52] to evaluate the efficacy of intramuscular moxifloxacin, as well as by Taghipour et al [7] in a comparative study of the effect of vancomycin, enrofloxacin, and trimethoprim/sulfamethoxazole. Bacterial inoculation is more often performed directly by intra-articular injection [33,53].…”

Section: Discussionmentioning

confidence: 99%

“…Our choice can be explained by the fact that intravenous drug administration in rabbits is extremely challenging due to the lack of available veins, and it is not possible to maintain an intravenous catheter for a long time in a vigilant rabbit. Moreover, intramuscular administration of antibiotics can result in peak serum concentrations within minutes at levels comparable to those observed after intravenous injections [52]. During the experiment, we monitored not only the bacterial load in the bone marrow but also body temperature and body weight.…”

Section: Discussionmentioning

confidence: 99%

The Antistaphylococcal Activity of Amoxicillin/Clavulanic Acid, Gentamicin, and 1,8‐Cineole Alone or in Combination and Their Efficacy through a Rabbit Model of Methicillin‐Resistant Staphylococcus aureus Osteomyelitis

Hriouech

Akhmouch

Mzabi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The aim of this research paper is to test the antistaphylococcal effect of 1,8-cineole, amoxicillin/clavulanic acid (AMC), and gentamicin, either separately or in combination against three Staphylococcus aureus strains isolated from patients suffering from osteomyelitis. This activity was tested in vitro by using the microdilution method and the checkerboard assay. The efficacy of these three antibacterial agents was then tested in vivo by using an experimental model of methicillin-resistant S. aureus osteomyelitis in rabbits. This efficacy was assessed after four days of treatment by counting the number of bacteria in the bone marrow. The obtained results in vitro showed that the combination of the AMC with gentamicin did not induce a synergistic effect, whereas the combination of the two antibiotics with 1,8-cineole did. This effect is stronger when AMC is combined with 1,8-cineole as a total synergistic effect was obtained on the three strains used (FIC ≤ 0.5). In vivo, a significant reduction was noted in the number of colonies in the bone marrow when rabbits were treated with AMC associated with either 1,8-cineole or gentamicin compared to rabbits treated with AMC, gentamicin, or 1,8-cineole alone. These results demonstrated that 1,8-cineole showed a synergistic effect in combination with both AMC and gentamicin, which offer possibilities for reducing antibiotic usage. Also, the AMC associated with 1,8-cineole could be used to treat MRSA osteomyelitis.

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“… 4 , 5 Owing to the broad resistance of MRSA, these infections have become more difficult to control and represent a significant public health challenge. 6 MRSA is present not only in hospitals but has also appeared in other environments and food; therefore, there are many more opportunities for individuals to come into contact with MRSA. 7 – 10 …”

Section: Introductionmentioning

confidence: 99%

Effect of MRSA on CYP450: dynamic changes of cytokines, oxidative stress, and drug-metabolizing enzymes in mice infected with MRSA

Tang¹,

Long²,

Lin³

et al. 2018

IDR

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BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) is a very damaging and widespread pathogen, which is associated with many diseases and causes serious infections. MRSA infection can modulate the effects of drugs, which may occur through an influence on cytochrome P450 (CYP450), the drug-metabolizing enzyme in the liver. In this study, we evaluated the underlying mechanism of drug failure or poisoning in MRSA infection.Materials and methodsMice were infected with three different doses of MRSA and the changes in CYP450 expression, cytokines, and oxidative stress markers were evaluated.ResultsThe administration of an attack dose of MRSA caused serious symptoms of infection and resulted in a 40% mortality rate in the mice. MRSA induced strong inflammation and oxidative stress in the mice, predominantly caused by significant increases in interleukin (IL)-1β, IL-4, IL-6, macrophage inflammatory protein, glutathione S-transferase (GST), and malondialdehyde, and decreases in oxygen radical absorbance capacity and glutathione levels in the liver. The expression of IL-2, tumor necrosis factor-α, and GST was briefly suppressed, but increased on days 3 and 7. The increased inflammation and oxidative stress further induced a significant decrease in the mRNA levels and activities of CYP450 1A2, 2D22, 2E1, and 3A1 in MRSA-infected mice within the first day of infection.ConclusionThese results show that MRSA infection leads to inflammation and oxidative stress, and reduces the expression levels and activities of drug metabolism enzymes, which decreased drug metabolism in patients infected with MRSA. Therefore, to avoid a drug overdose, the plasma concentration of patients with MRSA infection should be continuously monitored.

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Efficacy of intramuscular moxifloxacin in the treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus

Cited by 12 publications

References 30 publications

Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres

Preparation, in vitro release and antibacterial activity evaluation of rifampicin and moxifloxacin-loaded poly(D,L-lactide-co-glycolide) microspheres

The Antistaphylococcal Activity of Amoxicillin/Clavulanic Acid, Gentamicin, and 1,8‐Cineole Alone or in Combination and Their Efficacy through a Rabbit Model of Methicillin‐Resistant Staphylococcus aureus Osteomyelitis

Effect of MRSA on CYP450: dynamic changes of cytokines, oxidative stress, and drug-metabolizing enzymes in mice infected with MRSA

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