Efficacy of platelet-rich fibrin matrix on viability of diced cartilage grafts in a rabbit model

Güler, İsmail; Billur, Deniz; Aydin, Sevim; Kocatürk, Sinan

doi:10.1002/lary.25097

Cited by 33 publications

(19 citation statements)

References 38 publications

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“…Interestingly, the implantation of lyophilized PRFs has been shown to be more a potent remedy for craniofacial bone regeneration in mice and rats, possibly because of having up to 13‐fold larger pores compared to fresh PRFs (Li et al ., ). It has also been reported that PRFs could promote cartilage reconstruction in rabbits (Guler et al ., ), which adds to their list of advantages. These studies, when viewed together, support the view that the fibrin clot network can have an impact on a series of biological events at the fracture site, such as cytokine entrapment and cellular infiltration, thereby positively promoting different outcomes in bone healing.…”

Section: Prps and Prfs In Fracture Healingmentioning

confidence: 99%

Structural properties of fracture haematoma: current status and future clinical implications

Wang

Friis

Glatt

et al. 2016

J Tissue Eng Regen Med

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Blood clots (haematomas) that form immediately following a bone fracture have been shown to be vital for the subsequent healing process. During the clotting process, a number of factors can influence the fibrin clot structure, such as fibrin polymerization, growth factor binding, cellular infiltration (including platelet retraction), protein concentrations and cytokines. The modulation of the fibrin clot structure within the fracture site has important clinical implications and could result in the development of multifunctional scaffolds that mimic the natural structure of a haematoma. Artificial haematoma structures such as these can be created from the patient's own blood and can therefore act as an ideal bone defect filling material for potential clinical application to accelerate bone regeneration. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Prps and Prfs In Fracture Healingmentioning

confidence: 99%

Structural properties of fracture haematoma: current status and future clinical implications

Wang

Friis

Glatt

et al. 2016

J Tissue Eng Regen Med

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“…Additionally, appropriate negative pressure was maintained to promote cartilage growth and fusion. Previous studies have shown that the application of a “nougat” graft—diced cartilage combined with human fibrin glue—can greatly reduce the absorption of diced cartilage in the shaping of the nasal dorsum . In the present study, we used PRP to enhance the viability of chondrocytes and promote the production of an appropriate extracellular matrix .…”

Section: Discussionmentioning

confidence: 98%

Auricle shaping using 3D printing and autologous diced cartilage

Liao

Chen

et al. 2019

The Laryngoscope

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ObjectiveTo reconstruct the auricle using a porous, hollow, three‐dimensional (3D)‐printed mold and autologous diced cartilage mixed with platelet‐rich plasma (PRP).MethodsMaterialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5‐ to 2.0‐mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness.ResultsThe 3D‐printed auricle molds were 0.6‐mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P > 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans.ConclusionAuricle grafts were constructed successfully through packing a 3D‐printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties.Levels of EvidenceNA. Laryngoscope, 129:2467–2474, 2019

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“…The regenerative properties of the PRP added to the diced cartilage allow better integration of the grafts and reduce graft resorption. Güler [33] compared histologically the viability of chondrocytes, the content of collagen fiber in matrix and the peripheral tissue changes in four comparison groups of rabbits (bare diced cartilage/ diced cartilage wrapped in AlloDerm/ diced cartilage wrapped in Surgicel/ diced cartilage wrapped in platelet-rich fibrin matrix). Güler concluded that the viability of chondrocytes, the content of fiber in matrix and the peripheral tissue changes were higher in the cartilage embedded in the platelet-rich fibrin matrix group.…”

Section: Discussionmentioning

confidence: 99%

The Chrysalis Graft: Combination of Diced-Cartilage-Fascia Grafts and PRP in Augmentation Rhinoplasty

Guisantes¹

2019

IJTPS

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Introduction: This dorsal nasal augmentation technique utilizes a combination of the diced-cartilage with Platelet-Rich-Plasma (PRP), Platelet-Poor-Plasma (PPP) and fascia graft in a chrysalis form, which provides a regenerative and moldable technique. Methods: Patients requiring dorsal augmentation underwent rhinoplasty using diced-cartilagein a PRP-clot carrier, wrapped in a sleeve of deep-temporal fascia in achrysalis form. The PRP fraction was extracted after blood centrifugation and used to carry the diced-cartilage graft. The PPP and middle platelet layers were used as fibringlue sprayed over the chrysalis graft avoiding the use of stitches. Results: A total of 31 patients, with a mean age of 42 years old underwent augmentation rhinoplasty with this technique. The mean follow-up was 14 months. Donor sites for cartilage included nasal septum (n=13), concha (n=6), combination of both septum and concha (n=5) and rib (n=7). The surgical approach was open rhinoplasty in 20 cases and closed approach in 11 patients. No major complications requiring surgical revision were observed. No extrusions, no graft displacement and no skin irregularities were observed. Three cases (9.67%) of partial graft resorption were observed. Other complications included one case of local infection and two keloids in ear donor site. Satisfactory nasal dorsal augmentation was achieved in all other cases. Conclusions: This report describes a technique of dorsal augmentation using a mixture of diced-cartilage grafts, PRP, PPP and deep temporal fascia graft in a chrysalis manner. This approach offers a moldable and regenerative construct for nasal augmentation without major complications and minimal graft resorption.

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Efficacy of platelet-rich fibrin matrix on viability of diced cartilage grafts in a rabbit model

Abstract: We concluded that PRFM has significant advantages in ensuring the chondrocyte viability of diced cartilage grafts. It is also biocompatible, with relatively lesser inflammation and fibrosis.

Cited by 33 publications

References 38 publications

Structural properties of fracture haematoma: current status and future clinical implications

Structural properties of fracture haematoma: current status and future clinical implications

Auricle shaping using 3D printing and autologous diced cartilage

The Chrysalis Graft: Combination of Diced-Cartilage-Fascia Grafts and PRP in Augmentation Rhinoplasty

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