Efficacy of URP and ISSR markers to determine diversity of indigenous and exotic isolates of Curvularia lunata

Kumar, Pardeep; Akhtar, Jameel; Kandan, A.; Singh, Baleshwar; Kiran, Raj; Nair, K. S. S.; Dubey, S. C.

doi:10.1007/s42360-018-0038-7

Cited by 10 publications

(3 citation statements)

References 11 publications

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“…(Li et al., 2011), Fusarium oxysporum f. sp. ciceris (Dubey et al., 2012), Bipolaris oryzae (Kandan et al., 2015), Alternaria alternata (Bagherabadi et al., 2015), B. sorghicola (Kandan et al., 2016) and Curvularia lunata (Kumar et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

Dokhanchi

Arzanlou

Abed-Ashtiani

2021

Journal of Phytopathology

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Pyrenophora teres is an economically important pathogen of barley worldwide causing net blotch diseases. In this study, the frequency and mating-type alleles's distribution as well as genetic diversity of P. teres were assessed on micro-spatial scale and also on macro-geographical scale to find out whether a sexual cycle is possible within its Iranian populations. A multiplex PCR assay was developed for simultaneous identification and recognition of P. teres f. teres and P. teres f. maculata and to screen their mating-type alleles. 118 isolates of P. teres were procured from barley fields of East Azerbaijan province during 2017-2018. Among 89 isolates of P. teres f. teres examined, 39 isolates were found as MAT1-1 and 50 isolates were found to be MAT1-2.Among 29 isolates of P. teres f. maculata examined, 17 isolates were found as MAT1-1 and 12 isolates were found to be MAT1-2 with the dominancy belonging to MAT1-1.Nearly same mating-type alleles distribution within and between various populations of P. teres f. teres (1:1 ratio; χ 2 = 1.36) and P. teres f. maculata (1:1 ratio; χ 2 = 0.862) was observed. Genetic diversity assessment of 118 P. teres isolates was carried out through inter-simple sequence repeat (ISSR) analysis and results revealed that there was 70.83% and 63.89% polymorphism among the isolates of P. teres f. teres and P. teres f. maculata, respectively. No relation was observed between the isolates and their mating as well as geographical type. Among collected isolates (89 P. teres f. teres and 29 P. teres f. maculata isolates), the gene diversity was generally similar (h = 0.280 and h = 0.238 for P. teres f. teres and P. teres f. maculata, respectively). Based on our findings and referring to the prior data on the structure of P. teres population, we conclude that in the majority of tested areas, this fungal species might have the potential to undergo regular sexual recombination cycles.

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Section: Discussionmentioning

confidence: 99%

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

Dokhanchi

Arzanlou

Abed-Ashtiani

2021

Journal of Phytopathology

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“…Biochemical and molecular identification techniques have also been utilized to differentiate the virulence of C. lunata isolates. Isozymes, random amplified polymorphic DNA (RAPD), universally primed PCR (UP-PCR), internal simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) methods have also been tested to analyze the genetic variability among C. lunata strains. ,,,,− The classifications using one method or a combination of two or more methods were found to correlate with the classifications achieved using the differential host system method. Thus, biochemical and molecular identification methods have been used to assist the differential host system method in classifying the virulence of C. lunata .…”

Section: Virulence Differentiationmentioning

confidence: 99%

Curvularia lunataand Curvularia Leaf Spot of Maize in China

Wang

Liu

et al. 2022

ACS Omega

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Curvularia leaf spot (CLS), primarily caused by Curvularia lunata (Wakker) Boedijn (C. lunata), is widely distributed in maize production regions in China. It occurs in all the developmental stages of maize and causes economic losses. The epidemic and yield loss estimation models have been constructed for the disease. C. lunata has obvious virulence differentiation and produces multiple virulence factors. CLS is managed by application of chemical and biological agents and by quantitative resistance conferred by 5 to 6 quantitative trait loci (QTL). This review summarizes research on the understanding of CLS biological characteristics, virulence factors of C. lunata, host resistance genetics, and disease management strategies in China.

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“…But these are not significantly sufficient enough to accurately characterize various isolates into species. Now a days, molecular techniques are extensively used to resolve the issue of species level characterization in fungi (Caligiorne et al, 1999;Aggarwal et al, 2008;Kumar et al, 2018). Various works have characterized species of Bipolaris and Exserohilum from different crops based on morpho-molecular characterization (Bakonyi et al, 1995;Mehta, 2001;Weikert-Oliveira et al, 2002;Motlagh & Kaviani, 2008;Motlagh & Anvari, 2010;Kandan et al, 2014;Kandan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Morpho-molecular characterization of Bipolaris and Exserohilum spp. infecting various agricultural crops

Singh¹,

Sharma²,

Ali³

et al. 2021

IJAAS

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The study was undertaken with major objective to differentiate fungal isolates belonging to various species of Bipolaris and Exserohilum infecting various agricultural crops. After routine isolation and purification of more than 35 attempts, 13 isolates were morphologically characterized into 8 species namely, Bipolaris cynodontis (IQBC-1 and DQBC-2), B. holmii (IQBH-1), B. micropus (IQBM-1), B. oryzae (DQBO-1, DQBO-2), B. sorghicola (DQBS-1, DQBS-2), B. tetramera (DQBT-1, DQBT-2), Exserohilum longirostrata (IQEL-1) and E. rostratum (DQER-1 and DQER-2). Molecular characterization of all the isolates using RAPD, ISSR and URP markers also revealed genetic variation. Out of 24 RAPD primers, only 9 (OPA-2, OPA-4, OPA-9, OPB-4, OPB-8, OPC-1, OPC-2, OPC-5 and OPE-5) could amplify the DNA sequence showing good polymorphism. Out of 20 ISSR primers, only 6 (ISSR 1, ISSR 13, ISSR 15, ISSR 17, ISSR 19 and ISSR 20) could show good polymorphism. Out of 13 URPs, only 5 (URP 4R, URP 6R, URP 9F, URP 13R and URP 38F) could amplify with a greater number of bands. In all the three makers, similarity matrix value using Jaccard’s coefficient ranged from 0.149 to 0.548 revealing genetic co-relation among isolates. Combined clustering of RAPD, ISSR and URP data showed relatively low genetic similarity coefficient ranging from 14.92 to 54.76 among isolates which revealed stronger co-relation among isolates. Cluster analysis based on UPGMA provided a clear resolution of relationship among all the 13 isolates belong to 6 Bipolaris and 2 Exserohilum species and confirmed the morphological characterization of the same. Further, molecular markers especially, RAPD, ISSR and URP have revolutionized the genetic analysis of plant pathogens including fungi and their application can greatly help in characterizing related genera and delineation of inter and intra-species complex of fungi.

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Efficacy of URP and ISSR markers to determine diversity of indigenous and exotic isolates of Curvularia lunata

Cited by 10 publications

References 11 publications

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

Curvularia lunataand Curvularia Leaf Spot of Maize in China

Morpho-molecular characterization of Bipolaris and Exserohilum spp. infecting various agricultural crops

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